Detection of the single-stranded DNA of Streptomyces plasmid pSA1.1 and a binding histone-like protein

Eiji Yokoyama, Katsumi Doi, Makoto Kimura, Seiya Ogata

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Streptomyces plasmid pSA1.1 accumulated single-stranded DNA as replication intermediates in S. lividans; therefore, this plasmid was considered to replicate by a rolling-circle mechanism. A DNA-binding protein (pI > 9.7 and about 10 kDa) was purified on a denatured DNA-Cellulose column, then on a native DNA-Cellulose column. The N-terminal amino acid sequence of this protein has a high homology with bacterial histone-like proteins. In the gel retardation assay, this protein bound with the single-stranded DNA of pSA1.1. We propose that this protein may participate in the replication of pSA1.1.

Original languageEnglish
Pages (from-to)197-200
Number of pages4
JournalFEMS microbiology letters
Volume138
Issue number2-3
DOIs
Publication statusPublished - May 1 1996

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

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