Detection of the single-stranded DNA of Streptomyces plasmid pSA1.1 and a binding histone-like protein

Eiji Yokoyama, Katsumi Doi, Makoto Kimura, Seiya Ogata

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Streptomyces plasmid pSA1.1 accumulated single-stranded DNA as replication intermediates in S. lividans; therefore, this plasmid was considered to replicate by a rolling-circle mechanism. A DNA-binding protein (pI > 9.7 and about 10 kDa) was purified on a denatured DNA-Cellulose column, then on a native DNA-Cellulose column. The N-terminal amino acid sequence of this protein has a high homology with bacterial histone-like proteins. In the gel retardation assay, this protein bound with the single-stranded DNA of pSA1.1. We propose that this protein may participate in the replication of pSA1.1.

Original languageEnglish
Pages (from-to)197-200
Number of pages4
JournalFEMS microbiology letters
Volume138
Issue number2-3
DOIs
Publication statusPublished - May 1 1996

Fingerprint

Single-Stranded DNA
Streptomyces
Histones
Plasmids
Proteins
DNA-Binding Proteins
Electrophoretic Mobility Shift Assay
DNA Replication
Amino Acid Sequence
DNA-cellulose

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Detection of the single-stranded DNA of Streptomyces plasmid pSA1.1 and a binding histone-like protein. / Yokoyama, Eiji; Doi, Katsumi; Kimura, Makoto; Ogata, Seiya.

In: FEMS microbiology letters, Vol. 138, No. 2-3, 01.05.1996, p. 197-200.

Research output: Contribution to journalArticle

Yokoyama, Eiji ; Doi, Katsumi ; Kimura, Makoto ; Ogata, Seiya. / Detection of the single-stranded DNA of Streptomyces plasmid pSA1.1 and a binding histone-like protein. In: FEMS microbiology letters. 1996 ; Vol. 138, No. 2-3. pp. 197-200.
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