クワ暗斑病菌Myrothecium verrucariaの産生する毒性成分のクワ病葉からの検出と病原性に果たす役割について(平成17年度日本植物病理学会大会)

Translated title of the contribution: Detection of toxic substances from diseased mulberry leaves inoculated with Myrothecium verrucaria, cause of Myrothecium leaf spot of mulberry(The 2005 Annual Meeting of the Phytopathological Society of Japan in Shizuoka)

村上 理都子, 平舘 俊太郎, 白田 昭

Research output: Contribution to journalArticle

Abstract

Myrothecium verrucaria infects various plants, but the mechanism of infection is unknown. We studied infection of using M. verrucaria strain MAFF840074, that causes Myrothecium leaf spot of mulberry, to mulberry leaves. Infection by conidia washed with water was low, and an extract of the fungal culture grown on potato sucrose agar caused necroses of the leaves. Infection by conidia was high and the diameter of the resulting lesions was larger if the extract was added to the conidia at the time of inoculation. Roridin A, verrucarins A and J, and an unidentified toxic substance (toxic substance A) were isolated from the extract based on induction of necroses on mulberry leaves using high performance liquid chromatography (HPLC). Roridin A and verrucarin J were more toxic to mulberry leaves than verrucarin A and the toxic substance A. The toxicity of each substance tended to be higher on sensitive mulberry cultivars than on cultivars resistant to the disease. Toxic substances with the same Rf value as roridin A, verrucarin A and the toxic substance A using thin layer chromatography were detected from extracts of mulberry leaves after inoculation with the fungus. With HPLC, the substances in the extract and concentrations of them were determined to be highest (roridin A : 5×10^<-7>M, verrucarin A : 1.6×10^<-7>M and the toxic substance A : 21×10^<-7>M) at 12 days after the inoculation during the 14-d period. However, verrucarian J was not detected from the extract by the methods. Washed conidia of the fungus did not infect mulberry leaves (cv. Shin Ichinose) but infect the leaves when roridin A or verrucarin A at concentration that they caused necroces to the leaves or higher were added at the time of inoculation. Therefore, the results suggested that roridin A, verrucarin A and the toxic substance A might play some roles in the pathogenicity of the fungus.
Original languageJapanese
Pages (from-to)166-178
Number of pages13
Journal日本植物病理學會報
Volume71
Issue number3
Publication statusPublished - Aug 25 2005

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Myrothecium verrucaria
Myrothecium
leaf spot
roridins
mulberries
toxic substances
Japan
conidia
leaves
extracts
infection
fungi
necrosis
high performance liquid chromatography
lesions (plant)
cultivars
thin layer chromatography
pathogenicity
agar
potatoes

Cite this

@article{8a7aea8bfea2411a829658bb64d28660,
title = "クワ暗斑病菌Myrothecium verrucariaの産生する毒性成分のクワ病葉からの検出と病原性に果たす役割について(平成17年度日本植物病理学会大会)",
abstract = "Myrothecium verrucaria infects various plants, but the mechanism of infection is unknown. We studied infection of using M. verrucaria strain MAFF840074, that causes Myrothecium leaf spot of mulberry, to mulberry leaves. Infection by conidia washed with water was low, and an extract of the fungal culture grown on potato sucrose agar caused necroses of the leaves. Infection by conidia was high and the diameter of the resulting lesions was larger if the extract was added to the conidia at the time of inoculation. Roridin A, verrucarins A and J, and an unidentified toxic substance (toxic substance A) were isolated from the extract based on induction of necroses on mulberry leaves using high performance liquid chromatography (HPLC). Roridin A and verrucarin J were more toxic to mulberry leaves than verrucarin A and the toxic substance A. The toxicity of each substance tended to be higher on sensitive mulberry cultivars than on cultivars resistant to the disease. Toxic substances with the same Rf value as roridin A, verrucarin A and the toxic substance A using thin layer chromatography were detected from extracts of mulberry leaves after inoculation with the fungus. With HPLC, the substances in the extract and concentrations of them were determined to be highest (roridin A : 5×10^<-7>M, verrucarin A : 1.6×10^<-7>M and the toxic substance A : 21×10^<-7>M) at 12 days after the inoculation during the 14-d period. However, verrucarian J was not detected from the extract by the methods. Washed conidia of the fungus did not infect mulberry leaves (cv. Shin Ichinose) but infect the leaves when roridin A or verrucarin A at concentration that they caused necroces to the leaves or higher were added at the time of inoculation. Therefore, the results suggested that roridin A, verrucarin A and the toxic substance A might play some roles in the pathogenicity of the fungus.",
author = "理都子 村上 and 俊太郎 平舘 and 昭 白田",
year = "2005",
month = "8",
day = "25",
language = "Japanese",
volume = "71",
pages = "166--178",
journal = "日本植物病理學會報",
issn = "0031-9473",
publisher = "日本植物病理学会",
number = "3",

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TY - JOUR

T1 - クワ暗斑病菌Myrothecium verrucariaの産生する毒性成分のクワ病葉からの検出と病原性に果たす役割について(平成17年度日本植物病理学会大会)

AU - 村上, 理都子

AU - 平舘, 俊太郎

AU - 白田, 昭

PY - 2005/8/25

Y1 - 2005/8/25

N2 - Myrothecium verrucaria infects various plants, but the mechanism of infection is unknown. We studied infection of using M. verrucaria strain MAFF840074, that causes Myrothecium leaf spot of mulberry, to mulberry leaves. Infection by conidia washed with water was low, and an extract of the fungal culture grown on potato sucrose agar caused necroses of the leaves. Infection by conidia was high and the diameter of the resulting lesions was larger if the extract was added to the conidia at the time of inoculation. Roridin A, verrucarins A and J, and an unidentified toxic substance (toxic substance A) were isolated from the extract based on induction of necroses on mulberry leaves using high performance liquid chromatography (HPLC). Roridin A and verrucarin J were more toxic to mulberry leaves than verrucarin A and the toxic substance A. The toxicity of each substance tended to be higher on sensitive mulberry cultivars than on cultivars resistant to the disease. Toxic substances with the same Rf value as roridin A, verrucarin A and the toxic substance A using thin layer chromatography were detected from extracts of mulberry leaves after inoculation with the fungus. With HPLC, the substances in the extract and concentrations of them were determined to be highest (roridin A : 5×10^<-7>M, verrucarin A : 1.6×10^<-7>M and the toxic substance A : 21×10^<-7>M) at 12 days after the inoculation during the 14-d period. However, verrucarian J was not detected from the extract by the methods. Washed conidia of the fungus did not infect mulberry leaves (cv. Shin Ichinose) but infect the leaves when roridin A or verrucarin A at concentration that they caused necroces to the leaves or higher were added at the time of inoculation. Therefore, the results suggested that roridin A, verrucarin A and the toxic substance A might play some roles in the pathogenicity of the fungus.

AB - Myrothecium verrucaria infects various plants, but the mechanism of infection is unknown. We studied infection of using M. verrucaria strain MAFF840074, that causes Myrothecium leaf spot of mulberry, to mulberry leaves. Infection by conidia washed with water was low, and an extract of the fungal culture grown on potato sucrose agar caused necroses of the leaves. Infection by conidia was high and the diameter of the resulting lesions was larger if the extract was added to the conidia at the time of inoculation. Roridin A, verrucarins A and J, and an unidentified toxic substance (toxic substance A) were isolated from the extract based on induction of necroses on mulberry leaves using high performance liquid chromatography (HPLC). Roridin A and verrucarin J were more toxic to mulberry leaves than verrucarin A and the toxic substance A. The toxicity of each substance tended to be higher on sensitive mulberry cultivars than on cultivars resistant to the disease. Toxic substances with the same Rf value as roridin A, verrucarin A and the toxic substance A using thin layer chromatography were detected from extracts of mulberry leaves after inoculation with the fungus. With HPLC, the substances in the extract and concentrations of them were determined to be highest (roridin A : 5×10^<-7>M, verrucarin A : 1.6×10^<-7>M and the toxic substance A : 21×10^<-7>M) at 12 days after the inoculation during the 14-d period. However, verrucarian J was not detected from the extract by the methods. Washed conidia of the fungus did not infect mulberry leaves (cv. Shin Ichinose) but infect the leaves when roridin A or verrucarin A at concentration that they caused necroces to the leaves or higher were added at the time of inoculation. Therefore, the results suggested that roridin A, verrucarin A and the toxic substance A might play some roles in the pathogenicity of the fungus.

M3 - 記事

VL - 71

SP - 166

EP - 178

JO - 日本植物病理學會報

JF - 日本植物病理學會報

SN - 0031-9473

IS - 3

ER -