Detection of zearalenone using a metal-oxide-semiconductor field-effect-transistor-based biosensor employing a pt reference electrode

Byounghyun Lim, Byunghyun Cho, Jang Kyoo Shin, Ho Jin Choi, Sang Ho Seo, Sung Wook Choi, Hyang Sook Chun

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

We have fabricated a metal-oxide-semiconductor field-effect-transistor (MOSFET)-based biosensor for the detection of zearalenone using a standard complementary metal-oxide-semiconductor (CMOS) process. Au was used as the gate metal to immobilize a self-assembled monolayer (SAM) made of mercaptohexadecanoic acid (MHDA). The SAM was used to immobilize anti-zearalenone antibody. The carboxyl group of the SAM was bound to the anti-zearalenone antibody. Anti-zearalenone antibody and zearalenone were bound by an antigen-antibody reaction. The measurements were performed in phosphate buffered saline (PBS; pH 7.4) solution. A Pt electrode was employed as a reference electrode. The gate voltage of the sensor was applied using the Pt reference electrode. The binding of the SAM, anti-zearalenone antibody, and zearalenone caused a variation in the drain current of the MOSFET-based biosensor. To verify the interaction among the SAM, anti-zearalenone antibody, and zearalenone, surface plasmon resonance (SPR) measurements were performed.

Original languageEnglish
Pages (from-to)06FJ061-06FJ064
JournalJapanese journal of applied physics
Volume48
Issue number6 PART 2
DOIs
Publication statusPublished - Jun 2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Engineering(all)
  • Physics and Astronomy(all)

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