Determination of 1,5-anhydro-glucitol-carrier protein conjugates matrix-assisted laser desorption/ionization tof mass spectrometry and antibody formation

Li Jiang Xuan, Hiroyuki Tanaka, Satoshi Morimoto, Yukihiro Shoyama, Hiroshi Akanuma, Keiji Muraoka

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

In order to prepare the monoclonal antibody against 1,5-anhydro-glucitol (1), it was conjugated with bovine serum albumin (BSA), human serum albumin (HSA) or chicken lysozyme (CL) using succinate or β-alanine succinate as a spacer to produce individual antigen conjugates. The number of hapten contained in each antigen conjugate was determined by matrix-assisted laser desorption/ionization tof mass (MALDI-tof-MS) spectrometry. The formation of monoclonal antibody (MAb) was also discussed.

Original languageEnglish
Pages (from-to)85-92
Number of pages8
JournalSpectroscopy
Volume14
Issue number3
DOIs
Publication statusPublished - 2000

All Science Journal Classification (ASJC) codes

  • Spectroscopy

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