Determination of 1,5-anhydro-glucitol-carrier protein conjugates matrix-assisted laser desorption/ionization tof mass spectrometry and antibody formation

Li Jiang Xuan; Hiroyuki Tanaka; Satoshi Morimoto; Yukihiro Shoyama; Hiroshi Akanuma; Keiji Muraoka

doi:10.1155/2000/151704

Determination of 1,5-anhydro-glucitol-carrier protein conjugates matrix-assisted laser desorption/ionization tof mass spectrometry and antibody formation

Li Jiang Xuan, Hiroyuki Tanaka, Satoshi Morimoto, Yukihiro Shoyama, Hiroshi Akanuma, Keiji Muraoka

Pharmaceutical Health Care and Sciences

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

In order to prepare the monoclonal antibody against 1,5-anhydro-glucitol (1), it was conjugated with bovine serum albumin (BSA), human serum albumin (HSA) or chicken lysozyme (CL) using succinate or β-alanine succinate as a spacer to produce individual antigen conjugates. The number of hapten contained in each antigen conjugate was determined by matrix-assisted laser desorption/ionization tof mass (MALDI-tof-MS) spectrometry. The formation of monoclonal antibody (MAb) was also discussed.

Original language	English
Pages (from-to)	85-92
Number of pages	8
Journal	Spectroscopy
Volume	14
Issue number	3
DOIs	https://doi.org/10.1155/2000/151704
Publication status	Published - 2000

All Science Journal Classification (ASJC) codes

Spectroscopy

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10.1155/2000/151704

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abstract = "In order to prepare the monoclonal antibody against 1,5-anhydro-glucitol (1), it was conjugated with bovine serum albumin (BSA), human serum albumin (HSA) or chicken lysozyme (CL) using succinate or β-alanine succinate as a spacer to produce individual antigen conjugates. The number of hapten contained in each antigen conjugate was determined by matrix-assisted laser desorption/ionization tof mass (MALDI-tof-MS) spectrometry. The formation of monoclonal antibody (MAb) was also discussed.",

author = "Xuan, {Li Jiang} and Hiroyuki Tanaka and Satoshi Morimoto and Yukihiro Shoyama and Hiroshi Akanuma and Keiji Muraoka",

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doi = "10.1155/2000/151704",

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T1 - Determination of 1,5-anhydro-glucitol-carrier protein conjugates matrix-assisted laser desorption/ionization tof mass spectrometry and antibody formation

AU - Xuan, Li Jiang

AU - Tanaka, Hiroyuki

AU - Morimoto, Satoshi

AU - Shoyama, Yukihiro

AU - Akanuma, Hiroshi

AU - Muraoka, Keiji

PY - 2000

Y1 - 2000

N2 - In order to prepare the monoclonal antibody against 1,5-anhydro-glucitol (1), it was conjugated with bovine serum albumin (BSA), human serum albumin (HSA) or chicken lysozyme (CL) using succinate or β-alanine succinate as a spacer to produce individual antigen conjugates. The number of hapten contained in each antigen conjugate was determined by matrix-assisted laser desorption/ionization tof mass (MALDI-tof-MS) spectrometry. The formation of monoclonal antibody (MAb) was also discussed.

AB - In order to prepare the monoclonal antibody against 1,5-anhydro-glucitol (1), it was conjugated with bovine serum albumin (BSA), human serum albumin (HSA) or chicken lysozyme (CL) using succinate or β-alanine succinate as a spacer to produce individual antigen conjugates. The number of hapten contained in each antigen conjugate was determined by matrix-assisted laser desorption/ionization tof mass (MALDI-tof-MS) spectrometry. The formation of monoclonal antibody (MAb) was also discussed.

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Determination of 1,5-anhydro-glucitol-carrier protein conjugates matrix-assisted laser desorption/ionization tof mass spectrometry and antibody formation

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