Determination of aculeatisides based on immunoassay using a polyclonal antibody against aculeatiside A

Waraporn Putalun, Hiroyuki Tanaka, Toshiya Muranaka, Yukihiro Shoyama

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for determination of aculeatisides. Aculeatiside A was conjugated with bovine serum albumin (BSA) for immunization. The ratio of hapten in an antigen conjugate was determined by matrix-assisted laser desorption/ionization TOF mass spectrometry. Polyclonal antibody was developed in rabbits against an aculeatiside A-BSA conjugate. The antibody was specific for aculeatiside A and aculeatiside B. The range of the immunoassay extended from 100 ng ml-1 to 5 μg ml-1 of aculeatisides. Good correlation between ELISA and HPLC methods was obtained when crude extracts of plant samples were analyzed. The optimized ELISA was found to be applicable to the determination of total aculeatisides in various plant samples.

Original languageEnglish
Pages (from-to)1328-1332
Number of pages5
JournalAnalyst
Volume127
Issue number10
DOIs
Publication statusPublished - Oct 2002

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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