Determination of angiotensin metabolites in human plasma by fluorimetric high-performance liquid chromatography using a heart-cut column-switching technique

Toshiro Matsui, Kei Tamaya, Terukazu Kawasaki, Yutaka Osajima

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Fluorimetric column-switching HPLC method with naphthalene-2,3-dialdehyde (NDA) was developed for the determination of endogenous angiotensin (ANG) metabolites in human plasma. After one-step extraction to clean up the ultrafiltered plasma sample on the reversed HPLC system, the zone of the retention time of each ANG analyte was subjected to the NDA-derivatization. After putting into a first Phe-ODS (for ANG (3-4) and (5-8) determinations) or ODS column (for ANG I and II determinations), the heart-cut of the retention time of the NDA-ANG was separated on a second ODS column with a mobile phase containing 5 mM ion-pair reagent. Complete separation and good detection were accomplished within 2 h. Good linearity of the regression equation for all ANG analytes with the correlation coefficient of >0.993 as well as good reproducibility (C.V.<4.0%). Good agreement of the range of ANG II plasma level between the present (25-47 fmol/ml in plasma) and the radioimmunoassay methods (28-52 fmol/ml in plasma) indicated that the column-switching method could be applicable for the determination of endogenous smaller ANGs as well as for ANG I or II in plasma. Copyright (C) 1999.

Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume729
Issue number1-2
DOIs
Publication statusPublished - Jun 11 1999

Fingerprint

Plasma (human)
Angiotensins
High performance liquid chromatography
Metabolites
Plasmas
Angiotensin II
Angiotensin I
Ions
2,3-naphthalenedicarboxaldehyde

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

@article{b5b87bbaf7744c37aae679bfa808b030,
title = "Determination of angiotensin metabolites in human plasma by fluorimetric high-performance liquid chromatography using a heart-cut column-switching technique",
abstract = "Fluorimetric column-switching HPLC method with naphthalene-2,3-dialdehyde (NDA) was developed for the determination of endogenous angiotensin (ANG) metabolites in human plasma. After one-step extraction to clean up the ultrafiltered plasma sample on the reversed HPLC system, the zone of the retention time of each ANG analyte was subjected to the NDA-derivatization. After putting into a first Phe-ODS (for ANG (3-4) and (5-8) determinations) or ODS column (for ANG I and II determinations), the heart-cut of the retention time of the NDA-ANG was separated on a second ODS column with a mobile phase containing 5 mM ion-pair reagent. Complete separation and good detection were accomplished within 2 h. Good linearity of the regression equation for all ANG analytes with the correlation coefficient of >0.993 as well as good reproducibility (C.V.<4.0{\%}). Good agreement of the range of ANG II plasma level between the present (25-47 fmol/ml in plasma) and the radioimmunoassay methods (28-52 fmol/ml in plasma) indicated that the column-switching method could be applicable for the determination of endogenous smaller ANGs as well as for ANG I or II in plasma. Copyright (C) 1999.",
author = "Toshiro Matsui and Kei Tamaya and Terukazu Kawasaki and Yutaka Osajima",
year = "1999",
month = "6",
day = "11",
doi = "10.1016/S0378-4347(99)00128-0",
language = "English",
volume = "729",
pages = "89--95",
journal = "Journal of Chromatography B: Biomedical Sciences and Applications",
issn = "0378-4347",
publisher = "Elsevier BV",
number = "1-2",

}

TY - JOUR

T1 - Determination of angiotensin metabolites in human plasma by fluorimetric high-performance liquid chromatography using a heart-cut column-switching technique

AU - Matsui, Toshiro

AU - Tamaya, Kei

AU - Kawasaki, Terukazu

AU - Osajima, Yutaka

PY - 1999/6/11

Y1 - 1999/6/11

N2 - Fluorimetric column-switching HPLC method with naphthalene-2,3-dialdehyde (NDA) was developed for the determination of endogenous angiotensin (ANG) metabolites in human plasma. After one-step extraction to clean up the ultrafiltered plasma sample on the reversed HPLC system, the zone of the retention time of each ANG analyte was subjected to the NDA-derivatization. After putting into a first Phe-ODS (for ANG (3-4) and (5-8) determinations) or ODS column (for ANG I and II determinations), the heart-cut of the retention time of the NDA-ANG was separated on a second ODS column with a mobile phase containing 5 mM ion-pair reagent. Complete separation and good detection were accomplished within 2 h. Good linearity of the regression equation for all ANG analytes with the correlation coefficient of >0.993 as well as good reproducibility (C.V.<4.0%). Good agreement of the range of ANG II plasma level between the present (25-47 fmol/ml in plasma) and the radioimmunoassay methods (28-52 fmol/ml in plasma) indicated that the column-switching method could be applicable for the determination of endogenous smaller ANGs as well as for ANG I or II in plasma. Copyright (C) 1999.

AB - Fluorimetric column-switching HPLC method with naphthalene-2,3-dialdehyde (NDA) was developed for the determination of endogenous angiotensin (ANG) metabolites in human plasma. After one-step extraction to clean up the ultrafiltered plasma sample on the reversed HPLC system, the zone of the retention time of each ANG analyte was subjected to the NDA-derivatization. After putting into a first Phe-ODS (for ANG (3-4) and (5-8) determinations) or ODS column (for ANG I and II determinations), the heart-cut of the retention time of the NDA-ANG was separated on a second ODS column with a mobile phase containing 5 mM ion-pair reagent. Complete separation and good detection were accomplished within 2 h. Good linearity of the regression equation for all ANG analytes with the correlation coefficient of >0.993 as well as good reproducibility (C.V.<4.0%). Good agreement of the range of ANG II plasma level between the present (25-47 fmol/ml in plasma) and the radioimmunoassay methods (28-52 fmol/ml in plasma) indicated that the column-switching method could be applicable for the determination of endogenous smaller ANGs as well as for ANG I or II in plasma. Copyright (C) 1999.

UR - http://www.scopus.com/inward/record.url?scp=0032988493&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032988493&partnerID=8YFLogxK

U2 - 10.1016/S0378-4347(99)00128-0

DO - 10.1016/S0378-4347(99)00128-0

M3 - Article

C2 - 10410930

AN - SCOPUS:0032988493

VL - 729

SP - 89

EP - 95

JO - Journal of Chromatography B: Biomedical Sciences and Applications

JF - Journal of Chromatography B: Biomedical Sciences and Applications

SN - 0378-4347

IS - 1-2

ER -