TY - JOUR
T1 - Determination of antioxidant and anti-melanogenesis activities of Indonesian Lai, Durio kutejensis [Bombacaceae (Hassk) Becc] fruit extract
AU - Arung, Enos Tangke
AU - Suwinarti, Wiwin
AU - Hendra, Medi
AU - Supomo,
AU - Kusuma, Irawan Wijaya
AU - Puteri, Dwi Caesarina Nanda
AU - Eroglu, Huseyin Avni
AU - Kim, Yongung
AU - Shimizu, Kuniyoshi
AU - Ishikawa, Hiroya
N1 - Publisher Copyright:
© Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. All rights reserved.
Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Purpose: To investigate the antioxidant and anti-melanogenesis activities of Durio kutejensis [Bombacaceae (Hassk.) Becc] fruit extract. Methods: The fruit flesh of D. kutejensis was extracted successively with n-hexane, ethyl acetate/EtOAc, and ethanol/EtOH at room temperature repeatedly. The extracts were concentrated in vacuo to yield their residues. Antioxidant properties were analyzed by 2,2'-azino-bis(3- ethylbenzothiazoline-6-sulphonic acid) (ABTS, superoxide dismutase (SOD)-like activity, 2,2-diphenyl-1- picrylhydrazyl (DPPH), and oxygen radical absorbance capacity (ORAC) while anti-melanogensis activity was evaluated by tyrosinase enzyme activity and B16 melanoma cell assays (melanin inhibition and cytotoxicity). Results: The extract (200 μg/mL) showed melanin inhibition by inhibiting melanin formation in B16 melanoma cell by 47 % without cytotoxicity but did not inhibit tyrosinase enzyme activity. The extract (1 - 1000 μg/mL) also exhibited some level of antioxidant activity including ORAC (0.04 ± 0.00 μmol TE/mg at 950 μg/mL), ABTS (1.0 ± 0.2 % at 100.8 μg/mL), SOD (IC50, 76.00 ± 14.6 μg/mL, and DPPH (21.5 ± 0.7 % at 97.39 μg/mL extract concentration). Conclusion: The fruit extract of Durio kutejensis has antioxidant properties with a potential for treating hyperpigmentation and for use as a skin-lightening agent.
AB - Purpose: To investigate the antioxidant and anti-melanogenesis activities of Durio kutejensis [Bombacaceae (Hassk.) Becc] fruit extract. Methods: The fruit flesh of D. kutejensis was extracted successively with n-hexane, ethyl acetate/EtOAc, and ethanol/EtOH at room temperature repeatedly. The extracts were concentrated in vacuo to yield their residues. Antioxidant properties were analyzed by 2,2'-azino-bis(3- ethylbenzothiazoline-6-sulphonic acid) (ABTS, superoxide dismutase (SOD)-like activity, 2,2-diphenyl-1- picrylhydrazyl (DPPH), and oxygen radical absorbance capacity (ORAC) while anti-melanogensis activity was evaluated by tyrosinase enzyme activity and B16 melanoma cell assays (melanin inhibition and cytotoxicity). Results: The extract (200 μg/mL) showed melanin inhibition by inhibiting melanin formation in B16 melanoma cell by 47 % without cytotoxicity but did not inhibit tyrosinase enzyme activity. The extract (1 - 1000 μg/mL) also exhibited some level of antioxidant activity including ORAC (0.04 ± 0.00 μmol TE/mg at 950 μg/mL), ABTS (1.0 ± 0.2 % at 100.8 μg/mL), SOD (IC50, 76.00 ± 14.6 μg/mL, and DPPH (21.5 ± 0.7 % at 97.39 μg/mL extract concentration). Conclusion: The fruit extract of Durio kutejensis has antioxidant properties with a potential for treating hyperpigmentation and for use as a skin-lightening agent.
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U2 - 10.4314/tjpr.v14i1.7
DO - 10.4314/tjpr.v14i1.7
M3 - Article
AN - SCOPUS:84922021310
VL - 14
SP - 41
EP - 46
JO - Tropical Journal of Pharmaceutical Research
JF - Tropical Journal of Pharmaceutical Research
SN - 1596-5996
IS - 1
ER -