Determination of antioxidant and anti-melanogenesis activities of Indonesian Lai, Durio kutejensis [Bombacaceae (Hassk) Becc] fruit extract

Enos Tangke Arung, Wiwin Suwinarti, Medi Hendra, Supomo, Irawan Wijaya Kusuma, Dwi Caesarina Nanda Puteri, Huseyin Avni Eroglu, Yongung Kim, Kuniyoshi Shimizu, Hiroya Ishikawa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Purpose: To investigate the antioxidant and anti-melanogenesis activities of Durio kutejensis [Bombacaceae (Hassk.) Becc] fruit extract. Methods: The fruit flesh of D. kutejensis was extracted successively with n-hexane, ethyl acetate/EtOAc, and ethanol/EtOH at room temperature repeatedly. The extracts were concentrated in vacuo to yield their residues. Antioxidant properties were analyzed by 2,2'-azino-bis(3- ethylbenzothiazoline-6-sulphonic acid) (ABTS, superoxide dismutase (SOD)-like activity, 2,2-diphenyl-1- picrylhydrazyl (DPPH), and oxygen radical absorbance capacity (ORAC) while anti-melanogensis activity was evaluated by tyrosinase enzyme activity and B16 melanoma cell assays (melanin inhibition and cytotoxicity). Results: The extract (200 μg/mL) showed melanin inhibition by inhibiting melanin formation in B16 melanoma cell by 47 % without cytotoxicity but did not inhibit tyrosinase enzyme activity. The extract (1 - 1000 μg/mL) also exhibited some level of antioxidant activity including ORAC (0.04 ± 0.00 μmol TE/mg at 950 μg/mL), ABTS (1.0 ± 0.2 % at 100.8 μg/mL), SOD (IC50, 76.00 ± 14.6 μg/mL, and DPPH (21.5 ± 0.7 % at 97.39 μg/mL extract concentration). Conclusion: The fruit extract of Durio kutejensis has antioxidant properties with a potential for treating hyperpigmentation and for use as a skin-lightening agent.

Original languageEnglish
Pages (from-to)41-46
Number of pages6
JournalTropical Journal of Pharmaceutical Research
Volume14
Issue number1
DOIs
Publication statusPublished - Jan 1 2015

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Bombacaceae
Fruit
Melanins
Antioxidants
Experimental Melanomas
Monophenol Monooxygenase
Superoxide Dismutase
Hyperpigmentation
Sulfonic Acids
Enzymes
Inhibitory Concentration 50
Ethanol
Skin
Temperature

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science
  • Pharmacology (medical)

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Determination of antioxidant and anti-melanogenesis activities of Indonesian Lai, Durio kutejensis [Bombacaceae (Hassk) Becc] fruit extract. / Arung, Enos Tangke; Suwinarti, Wiwin; Hendra, Medi; Supomo; Kusuma, Irawan Wijaya; Puteri, Dwi Caesarina Nanda; Eroglu, Huseyin Avni; Kim, Yongung; Shimizu, Kuniyoshi; Ishikawa, Hiroya.

In: Tropical Journal of Pharmaceutical Research, Vol. 14, No. 1, 01.01.2015, p. 41-46.

Research output: Contribution to journalArticle

Arung, Enos Tangke ; Suwinarti, Wiwin ; Hendra, Medi ; Supomo ; Kusuma, Irawan Wijaya ; Puteri, Dwi Caesarina Nanda ; Eroglu, Huseyin Avni ; Kim, Yongung ; Shimizu, Kuniyoshi ; Ishikawa, Hiroya. / Determination of antioxidant and anti-melanogenesis activities of Indonesian Lai, Durio kutejensis [Bombacaceae (Hassk) Becc] fruit extract. In: Tropical Journal of Pharmaceutical Research. 2015 ; Vol. 14, No. 1. pp. 41-46.
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abstract = "Purpose: To investigate the antioxidant and anti-melanogenesis activities of Durio kutejensis [Bombacaceae (Hassk.) Becc] fruit extract. Methods: The fruit flesh of D. kutejensis was extracted successively with n-hexane, ethyl acetate/EtOAc, and ethanol/EtOH at room temperature repeatedly. The extracts were concentrated in vacuo to yield their residues. Antioxidant properties were analyzed by 2,2'-azino-bis(3- ethylbenzothiazoline-6-sulphonic acid) (ABTS, superoxide dismutase (SOD)-like activity, 2,2-diphenyl-1- picrylhydrazyl (DPPH), and oxygen radical absorbance capacity (ORAC) while anti-melanogensis activity was evaluated by tyrosinase enzyme activity and B16 melanoma cell assays (melanin inhibition and cytotoxicity). Results: The extract (200 μg/mL) showed melanin inhibition by inhibiting melanin formation in B16 melanoma cell by 47 {\%} without cytotoxicity but did not inhibit tyrosinase enzyme activity. The extract (1 - 1000 μg/mL) also exhibited some level of antioxidant activity including ORAC (0.04 ± 0.00 μmol TE/mg at 950 μg/mL), ABTS (1.0 ± 0.2 {\%} at 100.8 μg/mL), SOD (IC50, 76.00 ± 14.6 μg/mL, and DPPH (21.5 ± 0.7 {\%} at 97.39 μg/mL extract concentration). Conclusion: The fruit extract of Durio kutejensis has antioxidant properties with a potential for treating hyperpigmentation and for use as a skin-lightening agent.",
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T1 - Determination of antioxidant and anti-melanogenesis activities of Indonesian Lai, Durio kutejensis [Bombacaceae (Hassk) Becc] fruit extract

AU - Arung, Enos Tangke

AU - Suwinarti, Wiwin

AU - Hendra, Medi

AU - Supomo,

AU - Kusuma, Irawan Wijaya

AU - Puteri, Dwi Caesarina Nanda

AU - Eroglu, Huseyin Avni

AU - Kim, Yongung

AU - Shimizu, Kuniyoshi

AU - Ishikawa, Hiroya

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Purpose: To investigate the antioxidant and anti-melanogenesis activities of Durio kutejensis [Bombacaceae (Hassk.) Becc] fruit extract. Methods: The fruit flesh of D. kutejensis was extracted successively with n-hexane, ethyl acetate/EtOAc, and ethanol/EtOH at room temperature repeatedly. The extracts were concentrated in vacuo to yield their residues. Antioxidant properties were analyzed by 2,2'-azino-bis(3- ethylbenzothiazoline-6-sulphonic acid) (ABTS, superoxide dismutase (SOD)-like activity, 2,2-diphenyl-1- picrylhydrazyl (DPPH), and oxygen radical absorbance capacity (ORAC) while anti-melanogensis activity was evaluated by tyrosinase enzyme activity and B16 melanoma cell assays (melanin inhibition and cytotoxicity). Results: The extract (200 μg/mL) showed melanin inhibition by inhibiting melanin formation in B16 melanoma cell by 47 % without cytotoxicity but did not inhibit tyrosinase enzyme activity. The extract (1 - 1000 μg/mL) also exhibited some level of antioxidant activity including ORAC (0.04 ± 0.00 μmol TE/mg at 950 μg/mL), ABTS (1.0 ± 0.2 % at 100.8 μg/mL), SOD (IC50, 76.00 ± 14.6 μg/mL, and DPPH (21.5 ± 0.7 % at 97.39 μg/mL extract concentration). Conclusion: The fruit extract of Durio kutejensis has antioxidant properties with a potential for treating hyperpigmentation and for use as a skin-lightening agent.

AB - Purpose: To investigate the antioxidant and anti-melanogenesis activities of Durio kutejensis [Bombacaceae (Hassk.) Becc] fruit extract. Methods: The fruit flesh of D. kutejensis was extracted successively with n-hexane, ethyl acetate/EtOAc, and ethanol/EtOH at room temperature repeatedly. The extracts were concentrated in vacuo to yield their residues. Antioxidant properties were analyzed by 2,2'-azino-bis(3- ethylbenzothiazoline-6-sulphonic acid) (ABTS, superoxide dismutase (SOD)-like activity, 2,2-diphenyl-1- picrylhydrazyl (DPPH), and oxygen radical absorbance capacity (ORAC) while anti-melanogensis activity was evaluated by tyrosinase enzyme activity and B16 melanoma cell assays (melanin inhibition and cytotoxicity). Results: The extract (200 μg/mL) showed melanin inhibition by inhibiting melanin formation in B16 melanoma cell by 47 % without cytotoxicity but did not inhibit tyrosinase enzyme activity. The extract (1 - 1000 μg/mL) also exhibited some level of antioxidant activity including ORAC (0.04 ± 0.00 μmol TE/mg at 950 μg/mL), ABTS (1.0 ± 0.2 % at 100.8 μg/mL), SOD (IC50, 76.00 ± 14.6 μg/mL, and DPPH (21.5 ± 0.7 % at 97.39 μg/mL extract concentration). Conclusion: The fruit extract of Durio kutejensis has antioxidant properties with a potential for treating hyperpigmentation and for use as a skin-lightening agent.

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