Abstract
Purpose: To investigate the antioxidant and anti-melanogenesis activities of Durio kutejensis [Bombacaceae (Hassk.) Becc] fruit extract. Methods: The fruit flesh of D. kutejensis was extracted successively with n-hexane, ethyl acetate/EtOAc, and ethanol/EtOH at room temperature repeatedly. The extracts were concentrated in vacuo to yield their residues. Antioxidant properties were analyzed by 2,2'-azino-bis(3- ethylbenzothiazoline-6-sulphonic acid) (ABTS, superoxide dismutase (SOD)-like activity, 2,2-diphenyl-1- picrylhydrazyl (DPPH), and oxygen radical absorbance capacity (ORAC) while anti-melanogensis activity was evaluated by tyrosinase enzyme activity and B16 melanoma cell assays (melanin inhibition and cytotoxicity). Results: The extract (200 μg/mL) showed melanin inhibition by inhibiting melanin formation in B16 melanoma cell by 47 % without cytotoxicity but did not inhibit tyrosinase enzyme activity. The extract (1 - 1000 μg/mL) also exhibited some level of antioxidant activity including ORAC (0.04 ± 0.00 μmol TE/mg at 950 μg/mL), ABTS (1.0 ± 0.2 % at 100.8 μg/mL), SOD (IC50, 76.00 ± 14.6 μg/mL, and DPPH (21.5 ± 0.7 % at 97.39 μg/mL extract concentration). Conclusion: The fruit extract of Durio kutejensis has antioxidant properties with a potential for treating hyperpigmentation and for use as a skin-lightening agent.
| Original language | English |
|---|---|
| Pages (from-to) | 41-46 |
| Number of pages | 6 |
| Journal | Tropical Journal of Pharmaceutical Research |
| Volume | 14 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 2015 |
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All Science Journal Classification (ASJC) codes
- Pharmaceutical Science
- Pharmacology (medical)
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Determination of antioxidant and anti-melanogenesis activities of Indonesian Lai, Durio kutejensis [Bombacaceae (Hassk) Becc] fruit extract. / Arung, Enos Tangke; Suwinarti, Wiwin; Hendra, Medi; Supomo; Kusuma, Irawan Wijaya; Puteri, Dwi Caesarina Nanda; Eroglu, Huseyin Avni; Kim, Yongung; Shimizu, Kuniyoshi; Ishikawa, Hiroya.
In: Tropical Journal of Pharmaceutical Research, Vol. 14, No. 1, 01.01.2015, p. 41-46.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Determination of antioxidant and anti-melanogenesis activities of Indonesian Lai, Durio kutejensis [Bombacaceae (Hassk) Becc] fruit extract
AU - Arung, Enos Tangke
AU - Suwinarti, Wiwin
AU - Hendra, Medi
AU - Supomo,
AU - Kusuma, Irawan Wijaya
AU - Puteri, Dwi Caesarina Nanda
AU - Eroglu, Huseyin Avni
AU - Kim, Yongung
AU - Shimizu, Kuniyoshi
AU - Ishikawa, Hiroya
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Purpose: To investigate the antioxidant and anti-melanogenesis activities of Durio kutejensis [Bombacaceae (Hassk.) Becc] fruit extract. Methods: The fruit flesh of D. kutejensis was extracted successively with n-hexane, ethyl acetate/EtOAc, and ethanol/EtOH at room temperature repeatedly. The extracts were concentrated in vacuo to yield their residues. Antioxidant properties were analyzed by 2,2'-azino-bis(3- ethylbenzothiazoline-6-sulphonic acid) (ABTS, superoxide dismutase (SOD)-like activity, 2,2-diphenyl-1- picrylhydrazyl (DPPH), and oxygen radical absorbance capacity (ORAC) while anti-melanogensis activity was evaluated by tyrosinase enzyme activity and B16 melanoma cell assays (melanin inhibition and cytotoxicity). Results: The extract (200 μg/mL) showed melanin inhibition by inhibiting melanin formation in B16 melanoma cell by 47 % without cytotoxicity but did not inhibit tyrosinase enzyme activity. The extract (1 - 1000 μg/mL) also exhibited some level of antioxidant activity including ORAC (0.04 ± 0.00 μmol TE/mg at 950 μg/mL), ABTS (1.0 ± 0.2 % at 100.8 μg/mL), SOD (IC50, 76.00 ± 14.6 μg/mL, and DPPH (21.5 ± 0.7 % at 97.39 μg/mL extract concentration). Conclusion: The fruit extract of Durio kutejensis has antioxidant properties with a potential for treating hyperpigmentation and for use as a skin-lightening agent.
AB - Purpose: To investigate the antioxidant and anti-melanogenesis activities of Durio kutejensis [Bombacaceae (Hassk.) Becc] fruit extract. Methods: The fruit flesh of D. kutejensis was extracted successively with n-hexane, ethyl acetate/EtOAc, and ethanol/EtOH at room temperature repeatedly. The extracts were concentrated in vacuo to yield their residues. Antioxidant properties were analyzed by 2,2'-azino-bis(3- ethylbenzothiazoline-6-sulphonic acid) (ABTS, superoxide dismutase (SOD)-like activity, 2,2-diphenyl-1- picrylhydrazyl (DPPH), and oxygen radical absorbance capacity (ORAC) while anti-melanogensis activity was evaluated by tyrosinase enzyme activity and B16 melanoma cell assays (melanin inhibition and cytotoxicity). Results: The extract (200 μg/mL) showed melanin inhibition by inhibiting melanin formation in B16 melanoma cell by 47 % without cytotoxicity but did not inhibit tyrosinase enzyme activity. The extract (1 - 1000 μg/mL) also exhibited some level of antioxidant activity including ORAC (0.04 ± 0.00 μmol TE/mg at 950 μg/mL), ABTS (1.0 ± 0.2 % at 100.8 μg/mL), SOD (IC50, 76.00 ± 14.6 μg/mL, and DPPH (21.5 ± 0.7 % at 97.39 μg/mL extract concentration). Conclusion: The fruit extract of Durio kutejensis has antioxidant properties with a potential for treating hyperpigmentation and for use as a skin-lightening agent.
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U2 - 10.4314/tjpr.v14i1.7
DO - 10.4314/tjpr.v14i1.7
M3 - Article
VL - 14
SP - 41
EP - 46
JO - Tropical Journal of Pharmaceutical Research
JF - Tropical Journal of Pharmaceutical Research
SN - 1596-5996
IS - 1
ER -