Determination of azide in blood and urine by gas chromatography-mass spectrometry

Shigetoshi Kage, Keiko Kudo, Noriaki Ikeda

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

A sensitive and simple method for determining azide in blood and urine using an extractive alkylation technique was devised. This inorganic anion was alkylated with pentafluorobenzyl bromide using tetradecyldimethylbenzylammonium chloride as the phase-transfer catalyst. 1,3,5-Tribromobenzene was used as an internal standard. The obtained derivative was analyzed by gas chromatography-mass spectrometry using the negative ion chemical ionization mode with isobutane as the reagent gas. The calibration curves for azide were linear over the concentration range from 1 to 200 nmol/mL in blood and urine, and the lower limit of detection was 0.5 nmol/mL for blood and urine. The accuracy and precision of the method were evaluated, and the coefficients of variation were found to be lower than 10%.

Original languageEnglish
Pages (from-to)429-432
Number of pages4
JournalJournal of Analytical Toxicology
Volume24
Issue number6
DOIs
Publication statusPublished - 2000

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Chemical Health and Safety

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