Determination of baclofen and vigabatrin by microchip electrophoresis with fluorescence detection: Application of field-enhanced sample stacking and dynamic pH junction

Abdallah M. Zeid, Noritada Kaji, Jenny Jeehan M. Nasr, Fathalla Belal, Mohamed I. Walash, Yoshinobu Baba

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A simple, rapid and sensitive microchip electrophoretic (MCE) method with fluorescence detection is described for the simultaneous determination of two GABA analogue drugs, baclofen (BCN) and vigabatrin (VGN). Pre-microchip derivatization of both analytes with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) was performed in a basic borate buffer. The NBD-fluorescent derivatives of the studied drugs (λex/em 470/540) were baseline separated in a dynamically-coated poly(methyl methacrylate) microfluidic channel within 120 s using a 40 mM borate buffer containing 0.4% methylcellulose as the background solution. The ability of methylcellulose to form a network sieve of small pore size allowed the labelled analytes to be separated efficiently according to their molecular size variations with a resolution factor equal to 7.8 and a number of theoretical plates of more than 50 0000 per meter. The MCE method was applied to assay BCN and VGN in tablets using 6-aminohexanoic acid as an internal standard. The method sensitivity was enhanced by application of combined stacking and a dynamic pH junction. The method was applied to assay BCN in human plasma and human urine samples with a detection limit lower than 0.3 ng mL-1 and mean extraction recoveries of more than 95% (% RSD < 7) after protein precipitation with methanol.

Original languageEnglish
Pages (from-to)9965-9974
Number of pages10
JournalNew Journal of Chemistry
Volume42
Issue number12
DOIs
Publication statusPublished - Jan 1 2018

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Materials Chemistry

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