Determination of bisphosphonates by ion-pair HPLC

Łukasz Matuszewski; Anna Matuszewska; Tomasz Mazurkiewicz; Jerzy Rogalski; Nam Seok Cho; Shoji Ohga

Determination of bisphosphonates by ion-pair HPLC

Łukasz Matuszewski, Anna Matuszewska, Tomasz Mazurkiewicz, Jerzy Rogalski, Nam Seok Cho, Shoji Ohga

University Forest

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

The optimization of the two bisphosphonates (disodium pamidronate and zoledronic acid) separation by ion-suppression and ion-pair high-performance liquid chromatography is described. The influence of the eluent pH, composition of the mobile phase and the concentration of complexing ion-pairing agents was investigated. This developed method provides high separation and specificity to bisphosphonate analysis. In quantitative analysis in the optimized conditions (10mM n-amylamine or n-butylamine; 14% acetonitrile in 0.1 M sodium phosphate buffer, pH=7.0), the method showed good linearity (r=0.9999-0.9998) and sufficient sensitivity (10 ^-7-10 ^-8g/ml). It can be easily and conveniently adopted for the routine quality control analysis.

Original language	English
Pages (from-to)	213-216
Number of pages	4
Journal	Journal of the Faculty of Agriculture, Kyushu University
Volume	56
Issue number	2
Publication status	Published - Sep 2011

All Science Journal Classification (ASJC) codes

Biotechnology
Agronomy and Crop Science

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title = "Determination of bisphosphonates by ion-pair HPLC",

abstract = "The optimization of the two bisphosphonates (disodium pamidronate and zoledronic acid) separation by ion-suppression and ion-pair high-performance liquid chromatography is described. The influence of the eluent pH, composition of the mobile phase and the concentration of complexing ion-pairing agents was investigated. This developed method provides high separation and specificity to bisphosphonate analysis. In quantitative analysis in the optimized conditions (10mM n-amylamine or n-butylamine; 14% acetonitrile in 0.1 M sodium phosphate buffer, pH=7.0), the method showed good linearity (r=0.9999-0.9998) and sufficient sensitivity (10 -7-10 -8g/ml). It can be easily and conveniently adopted for the routine quality control analysis.",

author = "{\L}ukasz Matuszewski and Anna Matuszewska and Tomasz Mazurkiewicz and Jerzy Rogalski and Cho, {Nam Seok} and Shoji Ohga",

year = "2011",

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language = "English",

volume = "56",

pages = "213--216",

journal = "Journal of the Faculty of Agriculture, Kyushu University",

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T1 - Determination of bisphosphonates by ion-pair HPLC

AU - Matuszewski, Łukasz

AU - Matuszewska, Anna

AU - Mazurkiewicz, Tomasz

AU - Rogalski, Jerzy

AU - Cho, Nam Seok

AU - Ohga, Shoji

PY - 2011/9

Y1 - 2011/9

N2 - The optimization of the two bisphosphonates (disodium pamidronate and zoledronic acid) separation by ion-suppression and ion-pair high-performance liquid chromatography is described. The influence of the eluent pH, composition of the mobile phase and the concentration of complexing ion-pairing agents was investigated. This developed method provides high separation and specificity to bisphosphonate analysis. In quantitative analysis in the optimized conditions (10mM n-amylamine or n-butylamine; 14% acetonitrile in 0.1 M sodium phosphate buffer, pH=7.0), the method showed good linearity (r=0.9999-0.9998) and sufficient sensitivity (10 -7-10 -8g/ml). It can be easily and conveniently adopted for the routine quality control analysis.

AB - The optimization of the two bisphosphonates (disodium pamidronate and zoledronic acid) separation by ion-suppression and ion-pair high-performance liquid chromatography is described. The influence of the eluent pH, composition of the mobile phase and the concentration of complexing ion-pairing agents was investigated. This developed method provides high separation and specificity to bisphosphonate analysis. In quantitative analysis in the optimized conditions (10mM n-amylamine or n-butylamine; 14% acetonitrile in 0.1 M sodium phosphate buffer, pH=7.0), the method showed good linearity (r=0.9999-0.9998) and sufficient sensitivity (10 -7-10 -8g/ml). It can be easily and conveniently adopted for the routine quality control analysis.

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