Determination of bisphosphonates by ion-pair HPLC

Łukasz Matuszewski, Anna Matuszewska, Tomasz Mazurkiewicz, Jerzy Rogalski, Nam Seok Cho, Shoji Ohga

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Abstract

The optimization of the two bisphosphonates (disodium pamidronate and zoledronic acid) separation by ion-suppression and ion-pair high-performance liquid chromatography is described. The influence of the eluent pH, composition of the mobile phase and the concentration of complexing ion-pairing agents was investigated. This developed method provides high separation and specificity to bisphosphonate analysis. In quantitative analysis in the optimized conditions (10mM n-amylamine or n-butylamine; 14% acetonitrile in 0.1 M sodium phosphate buffer, pH=7.0), the method showed good linearity (r=0.9999-0.9998) and sufficient sensitivity (10 -7-10 -8g/ml). It can be easily and conveniently adopted for the routine quality control analysis.

Original languageEnglish
Pages (from-to)213-216
Number of pages4
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume56
Issue number2
Publication statusPublished - Sep 2011

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

Matuszewski, Ł., Matuszewska, A., Mazurkiewicz, T., Rogalski, J., Cho, N. S., & Ohga, S. (2011). Determination of bisphosphonates by ion-pair HPLC. Journal of the Faculty of Agriculture, Kyushu University, 56(2), 213-216.