Abstract
The optimization of the two bisphosphonates (disodium pamidronate and zoledronic acid) separation by ion-suppression and ion-pair high-performance liquid chromatography is described. The influence of the eluent pH, composition of the mobile phase and the concentration of complexing ion-pairing agents was investigated. This developed method provides high separation and specificity to bisphosphonate analysis. In quantitative analysis in the optimized conditions (10mM n-amylamine or n-butylamine; 14% acetonitrile in 0.1 M sodium phosphate buffer, pH=7.0), the method showed good linearity (r=0.9999-0.9998) and sufficient sensitivity (10 -7-10 -8g/ml). It can be easily and conveniently adopted for the routine quality control analysis.
| Original language | English |
|---|---|
| Pages (from-to) | 213-216 |
| Number of pages | 4 |
| Journal | Journal of the Faculty of Agriculture, Kyushu University |
| Volume | 56 |
| Issue number | 2 |
| Publication status | Published - Sep 2011 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Agronomy and Crop Science
Cite this
Matuszewski, Ł., Matuszewska, A., Mazurkiewicz, T., Rogalski, J., Cho, N. S., & Ohga, S. (2011). Determination of bisphosphonates by ion-pair HPLC. Journal of the Faculty of Agriculture, Kyushu University, 56(2), 213-216.