Determination of bromide in whole blood and urine from humans using gas chromatography-mass spectrometry

Shigetoshi Kage, Keiko Kudo, Hideaki Ikeda, Akira Tsujita, Noriaki Ikeda

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We devised a sensitive and simple method for determination of bromide in whole blood and urine from humans using gas chromatography-mass spectrometry. Bromide was alkylated with pentafluorobenzyl p-toluenesulphonate in the mixture of acetone and phosphate buffer (pH 6.8). The derivative obtained was analyzed using gas chromatography-mass spectrometry with the positive-ion EI mode. The lower limit of detection for the compound was 1 mg/l. The calibration curve for bromide was linear over the concentration range from 2 to 100 mg/l. With use of this method, levels of bromide in whole blood and urine were determined in cases of poisoning by inhaled brominated hydrocarbons.

Original languageEnglish
Pages (from-to)335-339
Number of pages5
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume817
Issue number2
DOIs
Publication statusPublished - Mar 25 2005

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Bromides
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Blood
Urine
Brominated Hydrocarbons
Acetone
Poisoning
Calibration
Limit of Detection
Buffers
Positive ions
Phosphates
Ions
Derivatives

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Cite this

Determination of bromide in whole blood and urine from humans using gas chromatography-mass spectrometry. / Kage, Shigetoshi; Kudo, Keiko; Ikeda, Hideaki; Tsujita, Akira; Ikeda, Noriaki.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 817, No. 2, 25.03.2005, p. 335-339.

Research output: Contribution to journalArticle

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