Abstract
An in vitro degradation test of angiotensin (ANG) II or III in normotensive supine human plasma from 9 healthy male subjects confirmed the production of smaller ANG metabolites with angiotensin I-converting enzyme inhibitory activity. These metabolites were identified as ANG (3–8), ANG (4–8), ANG (5–8), and ANG (3–4), whose respective peptide concentrations were determined by our proposed naphthalene-2, 3-dialdehyde (NDA)-HPLC method to be 64 ±9, 39 ±5, 176 ± 22, and 197 ± 35 fmol/ml of plasma. Pediococcus sp. ISK-1 isolated in our laboratory from well-aged Nukadoko, produces a bacteriocin which has a unique antimicrobial spectrum among pediocins. The bacteriocin was stable at acidic pH, and more than 60% of the antimicrobial activity still remained even after being autoclaved at 121°C for 20min in the pH range of 3 to 8. This is the first report dealing with a bacteriocin produced by lactic acid bacteria isolated from Nukadoko.
| Original language | English |
|---|---|
| Pages (from-to) | 1052-1054 |
| Number of pages | 3 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 61 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jan 1 1997 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry
Cite this
Determination of endogenous peptides with in vitro ACE inhibitory activity in normotensive human plasma by the fluorometric HPLC method. / Matsui, Toshiro; Matsufuji, Hiroshi; Kawasaki, Terukazu; Osajima, Yutaka.
In: Bioscience, Biotechnology and Biochemistry, Vol. 61, No. 6, 01.01.1997, p. 1052-1054.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Determination of endogenous peptides with in vitro ACE inhibitory activity in normotensive human plasma by the fluorometric HPLC method
AU - Matsui, Toshiro
AU - Matsufuji, Hiroshi
AU - Kawasaki, Terukazu
AU - Osajima, Yutaka
PY - 1997/1/1
Y1 - 1997/1/1
N2 - An in vitro degradation test of angiotensin (ANG) II or III in normotensive supine human plasma from 9 healthy male subjects confirmed the production of smaller ANG metabolites with angiotensin I-converting enzyme inhibitory activity. These metabolites were identified as ANG (3–8), ANG (4–8), ANG (5–8), and ANG (3–4), whose respective peptide concentrations were determined by our proposed naphthalene-2, 3-dialdehyde (NDA)-HPLC method to be 64 ±9, 39 ±5, 176 ± 22, and 197 ± 35 fmol/ml of plasma. Pediococcus sp. ISK-1 isolated in our laboratory from well-aged Nukadoko, produces a bacteriocin which has a unique antimicrobial spectrum among pediocins. The bacteriocin was stable at acidic pH, and more than 60% of the antimicrobial activity still remained even after being autoclaved at 121°C for 20min in the pH range of 3 to 8. This is the first report dealing with a bacteriocin produced by lactic acid bacteria isolated from Nukadoko.
AB - An in vitro degradation test of angiotensin (ANG) II or III in normotensive supine human plasma from 9 healthy male subjects confirmed the production of smaller ANG metabolites with angiotensin I-converting enzyme inhibitory activity. These metabolites were identified as ANG (3–8), ANG (4–8), ANG (5–8), and ANG (3–4), whose respective peptide concentrations were determined by our proposed naphthalene-2, 3-dialdehyde (NDA)-HPLC method to be 64 ±9, 39 ±5, 176 ± 22, and 197 ± 35 fmol/ml of plasma. Pediococcus sp. ISK-1 isolated in our laboratory from well-aged Nukadoko, produces a bacteriocin which has a unique antimicrobial spectrum among pediocins. The bacteriocin was stable at acidic pH, and more than 60% of the antimicrobial activity still remained even after being autoclaved at 121°C for 20min in the pH range of 3 to 8. This is the first report dealing with a bacteriocin produced by lactic acid bacteria isolated from Nukadoko.
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U2 - 10.1271/bbb.61.1052
DO - 10.1271/bbb.61.1052
M3 - Article
C2 - 9214772
AN - SCOPUS:0031170954
VL - 61
SP - 1052
EP - 1054
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 6
ER -