Determination of endogenous peptides with in vitro ACE inhibitory activity in normotensive human plasma by the fluorometric HPLC method

Toshiro Matsui, Hiroshi Matsufuji, Terukazu Kawasaki, Yutaka Osajima

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

An in vitro degradation test of angiotensin (ANG) II or III in normotensive supine human plasma from 9 healthy male subjects confirmed the production of smaller ANG metabolites with angiotensin I-converting enzyme inhibitory activity. These metabolites were identified as ANG (3–8), ANG (4–8), ANG (5–8), and ANG (3–4), whose respective peptide concentrations were determined by our proposed naphthalene-2, 3-dialdehyde (NDA)-HPLC method to be 64 ±9, 39 ±5, 176 ± 22, and 197 ± 35 fmol/ml of plasma. Pediococcus sp. ISK-1 isolated in our laboratory from well-aged Nukadoko, produces a bacteriocin which has a unique antimicrobial spectrum among pediocins. The bacteriocin was stable at acidic pH, and more than 60% of the antimicrobial activity still remained even after being autoclaved at 121°C for 20min in the pH range of 3 to 8. This is the first report dealing with a bacteriocin produced by lactic acid bacteria isolated from Nukadoko.

Original languageEnglish
Pages (from-to)1052-1054
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume61
Issue number6
DOIs
Publication statusPublished - Jan 1 1997

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Plasma (human)
Angiotensins
Bacteriocins
High Pressure Liquid Chromatography
Peptides
Metabolites
Angiotensin III
Pediococcus
Enzyme activity
Peptidyl-Dipeptidase A
Angiotensin II
Lactic Acid
Bacteria
Healthy Volunteers
In Vitro Techniques
Plasmas
Degradation

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Determination of endogenous peptides with in vitro ACE inhibitory activity in normotensive human plasma by the fluorometric HPLC method. / Matsui, Toshiro; Matsufuji, Hiroshi; Kawasaki, Terukazu; Osajima, Yutaka.

In: Bioscience, Biotechnology and Biochemistry, Vol. 61, No. 6, 01.01.1997, p. 1052-1054.

Research output: Contribution to journalArticle

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