Determination of fluoride in human whole blood and urine by gas chromatography-mass spectrometry

Shigetoshi Kage, Keiko Kudo, Naoki Nishida, Hideaki Ikeda, Naofumi Yoshioka, Noriaki Ikeda

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We developed a simple and sensitive method for determination of fluoride in human whole blood and urine using gas chromatography-mass spectrometry (GC-MS). Fluoride was alkylated with pentafluorobenzyl bromide in a mixture of acetone and phosphate buffer (pH 6.8). The derivative obtained was analyzed by GC-MS in the positive-ion electron-impact mode. The lower limit of detection for the compound was 0.5 mg/l for both matrices. The calibration curve for fluoride was linear over the concentration range of 1-100 mg/l. The precision and accuracy of the method were evaluated, and relative standard deviation was within 10%. Using this method, levels of fluoride in whole blood and urine were determined in a case of poisoning caused by hydrofluoric acid exposure.

Original languageEnglish
Pages (from-to)23-26
Number of pages4
JournalForensic Toxicology
Issue number1
Publication statusPublished - Jun 1 2008


All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Toxicology
  • Biochemistry, medical

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