Determination of guanine by high-performance liquid chromatography with electrochemical detection

Application to DNA and RNA assays

T. Nakahara, A. Shiraishi, M. Hirano, T. Matsumoto, Toshihide Kuroki, Y. Tatebayashi, T. Tsutsumi, K. Nishiyama, H. Ooboshi, K. Nakamura, H. Yao, M. Waki, H. Uchimura

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A highly sensitive assay for guanine was developed using high-performance liquid chromatography with electrochemical detection (ECD). Guanine was susceptible to the electrochemical oxidation, and ECD response was proportional to the amount of guanine in the range 0.25-4 pmol of guanine. The ECD of guanine was applicable to the analysis of nucleic acids. DNA and RNA were hydrolyzed in 0.03 and 3 M HCl, respectively, and guanine liberated from the nucleic acids was separated on a reverse-phase column and determined by ECD. The method allowed detection of 0.2 ng of calf thymus DNA or tRNA. An application of the method is shown for DNA and RNA assays in trichloroacetic acid extracts of rat adrenal and liver.

Original languageEnglish
Pages (from-to)38-42
Number of pages5
JournalAnalytical Biochemistry
Volume180
Issue number1
Publication statusPublished - Jan 1 1989

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Guanine
High performance liquid chromatography
Assays
High Pressure Liquid Chromatography
RNA
DNA
Nucleic Acids
Trichloroacetic Acid
Electrochemical oxidation
Transfer RNA
Liver
Rats

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Nakahara, T., Shiraishi, A., Hirano, M., Matsumoto, T., Kuroki, T., Tatebayashi, Y., ... Uchimura, H. (1989). Determination of guanine by high-performance liquid chromatography with electrochemical detection: Application to DNA and RNA assays. Analytical Biochemistry, 180(1), 38-42.

Determination of guanine by high-performance liquid chromatography with electrochemical detection : Application to DNA and RNA assays. / Nakahara, T.; Shiraishi, A.; Hirano, M.; Matsumoto, T.; Kuroki, Toshihide; Tatebayashi, Y.; Tsutsumi, T.; Nishiyama, K.; Ooboshi, H.; Nakamura, K.; Yao, H.; Waki, M.; Uchimura, H.

In: Analytical Biochemistry, Vol. 180, No. 1, 01.01.1989, p. 38-42.

Research output: Contribution to journalArticle

Nakahara, T, Shiraishi, A, Hirano, M, Matsumoto, T, Kuroki, T, Tatebayashi, Y, Tsutsumi, T, Nishiyama, K, Ooboshi, H, Nakamura, K, Yao, H, Waki, M & Uchimura, H 1989, 'Determination of guanine by high-performance liquid chromatography with electrochemical detection: Application to DNA and RNA assays', Analytical Biochemistry, vol. 180, no. 1, pp. 38-42.
Nakahara, T. ; Shiraishi, A. ; Hirano, M. ; Matsumoto, T. ; Kuroki, Toshihide ; Tatebayashi, Y. ; Tsutsumi, T. ; Nishiyama, K. ; Ooboshi, H. ; Nakamura, K. ; Yao, H. ; Waki, M. ; Uchimura, H. / Determination of guanine by high-performance liquid chromatography with electrochemical detection : Application to DNA and RNA assays. In: Analytical Biochemistry. 1989 ; Vol. 180, No. 1. pp. 38-42.
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