Determination of hybrids in Papaver species by RAPD, HPLC and ELISA

Y. Shoyama; H. Tanaka; S. Morimoto; F. Taura

Determination of hybrids in Papaver species by RAPD, HPLC and ELISA

Y. Shoyama, H. Tanaka, S. Morimoto, F. Taura

Pharmaceutical Health Care and Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Total DNA was extracted from the leaves of a F1 hybrid and its parents, Papaver bracteatum and P. pseudo-orientale. Analysis of RAPD using ten arbitrary oligonucleotide 10-mers, showed that F1 hybrid was confirmed to be genetically intermediate of both parental plants compared with the genetic distance between F1 hybrid and individual parents. Furthermore, the comparison of the band patterns between a F1 hybrid, P. bracteatum and P. pseudo-orientale clearly showed that part of the bands of both parents were induced into a F1 hybrid. The content of thebaine was determined by HPLC and ELISA used anti-thebaine monoclonal antibody.

Original language	English
Pages (from-to)	160-161
Number of pages	2
Journal	Japanese Journal of Forensic Toxicology
Volume	18
Issue number	2
Publication status	Published - 2000

All Science Journal Classification (ASJC) codes

Pathology and Forensic Medicine
Toxicology

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title = "Determination of hybrids in Papaver species by RAPD, HPLC and ELISA",

abstract = "Total DNA was extracted from the leaves of a F1 hybrid and its parents, Papaver bracteatum and P. pseudo-orientale. Analysis of RAPD using ten arbitrary oligonucleotide 10-mers, showed that F1 hybrid was confirmed to be genetically intermediate of both parental plants compared with the genetic distance between F1 hybrid and individual parents. Furthermore, the comparison of the band patterns between a F1 hybrid, P. bracteatum and P. pseudo-orientale clearly showed that part of the bands of both parents were induced into a F1 hybrid. The content of thebaine was determined by HPLC and ELISA used anti-thebaine monoclonal antibody.",

author = "Y. Shoyama and H. Tanaka and S. Morimoto and F. Taura",

year = "2000",

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pages = "160--161",

journal = "Forensic Toxicology",

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T1 - Determination of hybrids in Papaver species by RAPD, HPLC and ELISA

AU - Shoyama, Y.

AU - Tanaka, H.

AU - Morimoto, S.

AU - Taura, F.

PY - 2000

Y1 - 2000

N2 - Total DNA was extracted from the leaves of a F1 hybrid and its parents, Papaver bracteatum and P. pseudo-orientale. Analysis of RAPD using ten arbitrary oligonucleotide 10-mers, showed that F1 hybrid was confirmed to be genetically intermediate of both parental plants compared with the genetic distance between F1 hybrid and individual parents. Furthermore, the comparison of the band patterns between a F1 hybrid, P. bracteatum and P. pseudo-orientale clearly showed that part of the bands of both parents were induced into a F1 hybrid. The content of thebaine was determined by HPLC and ELISA used anti-thebaine monoclonal antibody.

AB - Total DNA was extracted from the leaves of a F1 hybrid and its parents, Papaver bracteatum and P. pseudo-orientale. Analysis of RAPD using ten arbitrary oligonucleotide 10-mers, showed that F1 hybrid was confirmed to be genetically intermediate of both parental plants compared with the genetic distance between F1 hybrid and individual parents. Furthermore, the comparison of the band patterns between a F1 hybrid, P. bracteatum and P. pseudo-orientale clearly showed that part of the bands of both parents were induced into a F1 hybrid. The content of thebaine was determined by HPLC and ELISA used anti-thebaine monoclonal antibody.

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