Determination of hybrids in Papaver species by RAPD, HPLC and ELISA

Y. Shoyama, H. Tanaka, Satoshi Morimoto, F. Taura

Research output: Contribution to journalArticle

Abstract

Total DNA was extracted from the leaves of a F1 hybrid and its parents, Papaver bracteatum and P. pseudo-orientale. Analysis of RAPD using ten arbitrary oligonucleotide 10-mers, showed that F1 hybrid was confirmed to be genetically intermediate of both parental plants compared with the genetic distance between F1 hybrid and individual parents. Furthermore, the comparison of the band patterns between a F1 hybrid, P. bracteatum and P. pseudo-orientale clearly showed that part of the bands of both parents were induced into a F1 hybrid. The content of thebaine was determined by HPLC and ELISA used anti-thebaine monoclonal antibody.

Original languageEnglish
Pages (from-to)160-161
Number of pages2
JournalJapanese Journal of Forensic Toxicology
Volume18
Issue number2
Publication statusPublished - 2000

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Thebaine
Papaver
Enzyme-Linked Immunosorbent Assay
High Pressure Liquid Chromatography
Oligonucleotides
Monoclonal Antibodies
DNA

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Toxicology

Cite this

Determination of hybrids in Papaver species by RAPD, HPLC and ELISA. / Shoyama, Y.; Tanaka, H.; Morimoto, Satoshi; Taura, F.

In: Japanese Journal of Forensic Toxicology, Vol. 18, No. 2, 2000, p. 160-161.

Research output: Contribution to journalArticle

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