Abstract
Total DNA was extracted from the leaves of a F1 hybrid and its parents, Papaver bracteatum and P. pseudo-orientale. Analysis of RAPD using ten arbitrary oligonucleotide 10-mers, showed that F1 hybrid was confirmed to be genetically intermediate of both parental plants compared with the genetic distance between F1 hybrid and individual parents. Furthermore, the comparison of the band patterns between a F1 hybrid, P. bracteatum and P. pseudo-orientale clearly showed that part of the bands of both parents were induced into a F1 hybrid. The content of thebaine was determined by HPLC and ELISA used anti-thebaine monoclonal antibody.
| Original language | English |
|---|---|
| Pages (from-to) | 160-161 |
| Number of pages | 2 |
| Journal | Japanese Journal of Forensic Toxicology |
| Volume | 18 |
| Issue number | 2 |
| Publication status | Published - 2000 |
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All Science Journal Classification (ASJC) codes
- Pathology and Forensic Medicine
- Toxicology
Cite this
Determination of hybrids in Papaver species by RAPD, HPLC and ELISA. / Shoyama, Y.; Tanaka, H.; Morimoto, Satoshi; Taura, F.
In: Japanese Journal of Forensic Toxicology, Vol. 18, No. 2, 2000, p. 160-161.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Determination of hybrids in Papaver species by RAPD, HPLC and ELISA
AU - Shoyama, Y.
AU - Tanaka, H.
AU - Morimoto, Satoshi
AU - Taura, F.
PY - 2000
Y1 - 2000
N2 - Total DNA was extracted from the leaves of a F1 hybrid and its parents, Papaver bracteatum and P. pseudo-orientale. Analysis of RAPD using ten arbitrary oligonucleotide 10-mers, showed that F1 hybrid was confirmed to be genetically intermediate of both parental plants compared with the genetic distance between F1 hybrid and individual parents. Furthermore, the comparison of the band patterns between a F1 hybrid, P. bracteatum and P. pseudo-orientale clearly showed that part of the bands of both parents were induced into a F1 hybrid. The content of thebaine was determined by HPLC and ELISA used anti-thebaine monoclonal antibody.
AB - Total DNA was extracted from the leaves of a F1 hybrid and its parents, Papaver bracteatum and P. pseudo-orientale. Analysis of RAPD using ten arbitrary oligonucleotide 10-mers, showed that F1 hybrid was confirmed to be genetically intermediate of both parental plants compared with the genetic distance between F1 hybrid and individual parents. Furthermore, the comparison of the band patterns between a F1 hybrid, P. bracteatum and P. pseudo-orientale clearly showed that part of the bands of both parents were induced into a F1 hybrid. The content of thebaine was determined by HPLC and ELISA used anti-thebaine monoclonal antibody.
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M3 - Article
AN - SCOPUS:0034044860
VL - 18
SP - 160
EP - 161
JO - Forensic Toxicology
JF - Forensic Toxicology
SN - 1860-8965
IS - 2
ER -