Determination of Nerve Agent Metabolites by Ultraviolet Femtosecond Laser Ionization Mass Spectrometry

Akifumi Hamachi, Tomoko Imasaka, Hiroshi Nakamura, Adan Li, Totaro Imasaka

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Nerve agent metabolites, i.e., isopropyl methylphosphonic acid (IMPA) and pinacolyl methylphosphonic acid (PMPA), were derivatized by reacting them with 2,3,4,5,6-pentafluorobenzyl bromide (PFBBr) and were determined by mass spectrometry using an ultraviolet femtosecond laser emitting at 267 and 200 nm as the ionization source. The analytes of the derivatized compounds, i.e., IMPA-PFB and PMPA-PFB, contain a large side-chain, and molecular ions are very weak or absent in electron ionization mass spectrometry. The use of ultraviolet femtosecond laser ionization mass spectrometry, however, resulted in the formation of a molecular ion, even for compounds such as these that contain a highly bulky functional group. The signal intensity was larger at 200 nm due to resonance-enhanced two-photon ionization. In contrast, fragmentation was suppressed at 267 nm (nonresonant two-photon ionization) especially for PMPA-PFB, thus resulting in a lower background signal. This favorable result can be explained by the small excess energy in ionization at 267 nm and by the low-frequency vibrational mode of a bulky trimethylpropyl group in PMPA.

Original languageEnglish
Pages (from-to)5030-5035
Number of pages6
JournalAnalytical Chemistry
Volume89
Issue number9
DOIs
Publication statusPublished - May 2 2017

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Metabolites
Ultrashort pulses
Ionization
Mass spectrometry
Photons
Ions
Vibrational spectra
Ion sources
Functional groups
Nerve Agents
pinacolyl methylphosphonic acid
Electrons
isopropyl methylphosphonic acid

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Determination of Nerve Agent Metabolites by Ultraviolet Femtosecond Laser Ionization Mass Spectrometry. / Hamachi, Akifumi; Imasaka, Tomoko; Nakamura, Hiroshi; Li, Adan; Imasaka, Totaro.

In: Analytical Chemistry, Vol. 89, No. 9, 02.05.2017, p. 5030-5035.

Research output: Contribution to journalArticle

Hamachi, Akifumi ; Imasaka, Tomoko ; Nakamura, Hiroshi ; Li, Adan ; Imasaka, Totaro. / Determination of Nerve Agent Metabolites by Ultraviolet Femtosecond Laser Ionization Mass Spectrometry. In: Analytical Chemistry. 2017 ; Vol. 89, No. 9. pp. 5030-5035.
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