Determination of oxycodone and hydrocotarnine in cancer patient serum by high-performance liquid chromatography with electrochemical detection

Hideya Kokubun, Makiko Ouki, Motohiro Matoba, Hiroaki Kubo, Sumio Hoka, Kazuo Yago

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

We developed an HPLC procedure using electrochemical detection for the quantitation of oxycodone and hydrocotamine in cancer patients serum. An eluent of methanol:acetonitrile:5 mM pH 8 phosphate buffer (2:1:7) was used for the mobile phase. The calibration curve was linear in the range from 10 ng/mL to 100 ng/mL. The recovery of oxycodone and hydrocotarnine was 97.2% and 90.5%, respectively. The relative standard deviations within-runs and between-runs for the assay of oxycodone or hydrocotamine were less than 4.8%. The method developed here was better than the method reported previously. 2005

Original languageEnglish
Pages (from-to)337-339
Number of pages3
Journalanalytical sciences
Volume21
Issue number3
DOIs
Publication statusPublished - Mar 2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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