Determination of oxycodone and hydrocotarnine in cancer patient serum by high-performance liquid chromatography with electrochemical detection

Hideya Kokubun, Makiko Ouki, Motohiro Matoba, Hiroaki Kubo, Sumio Hoka, Kazuo Yago

Research output: Contribution to journalArticle

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Abstract

We developed an HPLC procedure using electrochemical detection for the quantitation of oxycodone and hydrocotamine in cancer patients serum. An eluent of methanol:acetonitrile:5 mM pH 8 phosphate buffer (2:1:7) was used for the mobile phase. The calibration curve was linear in the range from 10 ng/mL to 100 ng/mL. The recovery of oxycodone and hydrocotarnine was 97.2% and 90.5%, respectively. The relative standard deviations within-runs and between-runs for the assay of oxycodone or hydrocotamine were less than 4.8%. The method developed here was better than the method reported previously. 2005

Original languageEnglish
Pages (from-to)337-339
Number of pages3
Journalanalytical sciences
Volume21
Issue number3
DOIs
Publication statusPublished - Mar 2005

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Oxycodone
High performance liquid chromatography
Methanol
Assays
Buffers
Phosphates
Calibration
Recovery
hydrocotarnine

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Determination of oxycodone and hydrocotarnine in cancer patient serum by high-performance liquid chromatography with electrochemical detection. / Kokubun, Hideya; Ouki, Makiko; Matoba, Motohiro; Kubo, Hiroaki; Hoka, Sumio; Yago, Kazuo.

In: analytical sciences, Vol. 21, No. 3, 03.2005, p. 337-339.

Research output: Contribution to journalArticle

Kokubun, Hideya ; Ouki, Makiko ; Matoba, Motohiro ; Kubo, Hiroaki ; Hoka, Sumio ; Yago, Kazuo. / Determination of oxycodone and hydrocotarnine in cancer patient serum by high-performance liquid chromatography with electrochemical detection. In: analytical sciences. 2005 ; Vol. 21, No. 3. pp. 337-339.
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