TY - JOUR
T1 - Determination of polysulphides in blood by gas chromatography and gas chromatography-mass spectrometry
AU - Kage, S.
AU - Nagata, T.
AU - Kudo, K.
PY - 1991/3/8
Y1 - 1991/3/8
N2 - A sensitive and simple method to determine polysulphides in human blood, using an extractive alkylation technique and gas chromatography, has been devised. Polysulphides were alkylated with pentafluorobenzyl bromide, and then converted into bis(pentafluorobenzyl)disulphide by desulphuration with potassium cyanide. The disulphide was analysed qualitatively by mass fragmentography and quantitatively by gas chromatography with electron-capture detection. The lower limit of detection was 0.005 μmol/ml. Field testing in a suicide case confirmed the validity of the method.
AB - A sensitive and simple method to determine polysulphides in human blood, using an extractive alkylation technique and gas chromatography, has been devised. Polysulphides were alkylated with pentafluorobenzyl bromide, and then converted into bis(pentafluorobenzyl)disulphide by desulphuration with potassium cyanide. The disulphide was analysed qualitatively by mass fragmentography and quantitatively by gas chromatography with electron-capture detection. The lower limit of detection was 0.005 μmol/ml. Field testing in a suicide case confirmed the validity of the method.
UR - http://www.scopus.com/inward/record.url?scp=0025973818&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025973818&partnerID=8YFLogxK
U2 - 10.1016/0378-4347(91)80078-Q
DO - 10.1016/0378-4347(91)80078-Q
M3 - Article
C2 - 1860910
AN - SCOPUS:0025973818
SN - 0378-4347
VL - 564
SP - 163
EP - 169
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - 1
ER -
