Determination of polysulphides in blood by gas chromatography and gas chromatography-mass spectrometry

S. Kage, T. Nagata, K. Kudo

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A sensitive and simple method to determine polysulphides in human blood, using an extractive alkylation technique and gas chromatography, has been devised. Polysulphides were alkylated with pentafluorobenzyl bromide, and then converted into bis(pentafluorobenzyl)disulphide by desulphuration with potassium cyanide. The disulphide was analysed qualitatively by mass fragmentography and quantitatively by gas chromatography with electron-capture detection. The lower limit of detection was 0.005 μmol/ml. Field testing in a suicide case confirmed the validity of the method.

Original languageEnglish
Pages (from-to)163-169
Number of pages7
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume564
Issue number1
DOIs
Publication statusPublished - Mar 8 1991

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

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