Determination of pseudojujubogenin glycosides from Brahmi based on immunoassay using a monoclonal antibody against bacopaside I

Watoo Phrompittayarat, Waraporn Putalun, Hiroyuki Tanaka, Kanchalee Jetiyanon, Sakchai Wlttaya-Areekul, Kornkanok Ingkaninan

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Bacopa monnieri (L.) Wettst. (Brahmi) is an Ayurvedic medicinal plant used for centuries as a memory enhancer. Dammarane-type triterpenoid saponins classified as pseudojujubogenin and jujubogenin glycosides are reported as the active components in this plant. In this study, the monoclonal antibody (MAb) against bacopaside I, a major pseudojujubogenin glycoside found in Brahmi, was produced and characterised. Bacopaside I was conjugated with bovine albumin serum to prepare an immunogen. Hybridomas secreting a monoclonal antibody against bacopaside I was produced by fusing splenocytes from an immunised mouse and SP2/0-Ag14 myeloma cells. The results showed that the antibodies were raised specifically against pseudojujubonin glycosides. An enzyme-linked immunosorbant assay (ELISA) using anti-bacopaside I MAb was performed in the range of 1.95-62.5 ng/mL of bacopaside I, with a limit of detection of 0.5 ng/mL. The method was validated and the practical use of the ELISA for analysing saponin glycosides from Brahmi was demonstrated.

Original languageEnglish
Pages (from-to)411-418
Number of pages8
JournalPhytochemical Analysis
Volume18
Issue number5
DOIs
Publication statusPublished - Sep 1 2007

Fingerprint

Glycosides
immunoassays
Immunoassay
glycosides
monoclonal antibodies
Monoclonal Antibodies
Saponins
Bacopa monnieri
Assays
Bacopa
myeloma
triterpenoid saponins
hybridomas
assays
Plant Structures
bovine serum albumin
splenocytes
enzymes
saponins
medicinal plants

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Food Science
  • Biochemistry
  • Molecular Medicine
  • Plant Science
  • Drug Discovery
  • Complementary and alternative medicine

Cite this

Phrompittayarat, W., Putalun, W., Tanaka, H., Jetiyanon, K., Wlttaya-Areekul, S., & Ingkaninan, K. (2007). Determination of pseudojujubogenin glycosides from Brahmi based on immunoassay using a monoclonal antibody against bacopaside I. Phytochemical Analysis, 18(5), 411-418. https://doi.org/10.1002/pca.996

Determination of pseudojujubogenin glycosides from Brahmi based on immunoassay using a monoclonal antibody against bacopaside I. / Phrompittayarat, Watoo; Putalun, Waraporn; Tanaka, Hiroyuki; Jetiyanon, Kanchalee; Wlttaya-Areekul, Sakchai; Ingkaninan, Kornkanok.

In: Phytochemical Analysis, Vol. 18, No. 5, 01.09.2007, p. 411-418.

Research output: Contribution to journalArticle

Phrompittayarat, W, Putalun, W, Tanaka, H, Jetiyanon, K, Wlttaya-Areekul, S & Ingkaninan, K 2007, 'Determination of pseudojujubogenin glycosides from Brahmi based on immunoassay using a monoclonal antibody against bacopaside I', Phytochemical Analysis, vol. 18, no. 5, pp. 411-418. https://doi.org/10.1002/pca.996
Phrompittayarat, Watoo ; Putalun, Waraporn ; Tanaka, Hiroyuki ; Jetiyanon, Kanchalee ; Wlttaya-Areekul, Sakchai ; Ingkaninan, Kornkanok. / Determination of pseudojujubogenin glycosides from Brahmi based on immunoassay using a monoclonal antibody against bacopaside I. In: Phytochemical Analysis. 2007 ; Vol. 18, No. 5. pp. 411-418.
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