Determination of tetrahydrocannabinolic acid—carrier protein conjugate by matrix‐assisted laser desorption/ionization mass spectrometry and antibody formation

Yuri Goto, Yumi Shima, Satoshi Morimoto, Yukihiro Shoyama, Hiroki Murakami, Akihiko Kusai, Kazutetsu Nojima

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

In order to form the monoclonal antibody against tetrahydrocannabinolic acid (THCA), THCA was coupled with bovine serum albumin (BSA) or human serum albumin (HSA) using β‐alanine as a linker or without β‐alanine to produce individual antigen conjugates. The number of haptens contained in the antigen conjugates was determined by matrix‐assisted laser desorption ionization mass spectrometry using BSA or HSA as an internal standard. The formation of monoclonal antibody is also discussed.

Original languageEnglish
Pages (from-to)668-671
Number of pages4
JournalOrganic Mass Spectrometry
Volume29
Issue number11
DOIs
Publication statusPublished - Jan 1 1994

Fingerprint

Monoclonal antibodies
Antigens
Bovine Serum Albumin
antibodies
albumins
Antibodies
Serum Albumin
Alanine
serums
Ionization
Antibody Formation
Mass spectrometry
Desorption
Mass Spectrometry
Lasers
mass spectroscopy
desorption
Monoclonal Antibodies
proteins
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Instrumentation

Cite this

Determination of tetrahydrocannabinolic acid—carrier protein conjugate by matrix‐assisted laser desorption/ionization mass spectrometry and antibody formation. / Goto, Yuri; Shima, Yumi; Morimoto, Satoshi; Shoyama, Yukihiro; Murakami, Hiroki; Kusai, Akihiko; Nojima, Kazutetsu.

In: Organic Mass Spectrometry, Vol. 29, No. 11, 01.01.1994, p. 668-671.

Research output: Contribution to journalArticle

Goto, Yuri ; Shima, Yumi ; Morimoto, Satoshi ; Shoyama, Yukihiro ; Murakami, Hiroki ; Kusai, Akihiko ; Nojima, Kazutetsu. / Determination of tetrahydrocannabinolic acid—carrier protein conjugate by matrix‐assisted laser desorption/ionization mass spectrometry and antibody formation. In: Organic Mass Spectrometry. 1994 ; Vol. 29, No. 11. pp. 668-671.
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