Determination of the complete cDNA sequence, construction of expression systems, and elucidation of fibrinolytic activity for Tapes japonica lysozyme

Kouhei Takeshita, Yoshio Hashimoto, Yoshiyuki Thujihata, Takanori So, Tadashi Ueda, Taiji Iomoto

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The lysozyme of the marine bivalve, Tapes japonica (13.8kDa), belongs to the invertebrate lysozyme family and displays both chitinase and isopeptidase activities. We determined the complete cDNA sequence and constructed effective expression systems for this enzyme using Escherichia coli (BL21) and Pichia pastoris. The native and recombinant proteins indicated lysozyme activity and isopeptidase activity, including the proteolysis of D-dimer, a plasminolytic product of stabilized polymeric fibrin. These results will be utilized for the structural and functional study of invertebrate lysozymes, and for the development of applications for thrombosis therapies.

Original languageEnglish
Pages (from-to)254-262
Number of pages9
JournalProtein Expression and Purification
Volume36
Issue number2
DOIs
Publication statusPublished - Aug 1 2004

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All Science Journal Classification (ASJC) codes

  • Biotechnology

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