Determination of thick and thin fibres distribution in Sunda porcupine dorsal skin (Hystrix javanica) using Picrosirius red staining

Andhika Yudha Prawira, Savitri Novelina, Wartika Rosa Farida, Huda Shalahudin Darusman, Katsuhiko Warita, Yoshinao Z. Hosaka, Srihadi Agungpriyono

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The complexity of the Sunda porcupine skin has become an important topic due to the unique characteristics of its quill follicles. The structure and chemical composition of the skin has affected many physiological and other conditions. Generally, quills are larger, stronger and stiffer than hair; therefore, the skin structure needs to adapt to support their physiology. The strength of the skin is determined by its collagen composition and arrangement; therefore, this study aims to analyse the composition and distribution of thick and thin fibres based on the specific characteristics of Sunda porcupine skin under polarized light using picrosirius red staining. The skin samples used were from the thoracodorsal and lumbosacral regions of eight Sunda porcupine adults. The histological staining was carried out using the picrosirius red method, while the samples were observed under a polarized light microscope and analysed with software. The results showed that the skin is composed of 36%–65% thick fibres, 20%–35% thin fibres and small amounts of other types with the lumbosacral region having higher compositions of thick and thin fibres than those in the thoracodorsal region. Furthermore, the thoracodorsal and lumbosacral regions have the highest composition of thick fibre in the deeper dermis and quill follicle, respectively. These demonstrated that the complexity of the skin structure of Sunda porcupine due to its quill derivates correlated with its collagen composition and distribution.

Original languageEnglish
Pages (from-to)666-673
Number of pages8
JournalJournal of Veterinary Medicine Series C: Anatomia Histologia Embryologia
Volume51
Issue number5
DOIs
Publication statusPublished - Sep 2022
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • veterinary(all)

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