Determination of thiosulfate in body fluids by GC and GC/MS

S. Kage, T. Nagata, K. Kudo

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Abstract

A simple and sensitive method to determine thiosulfate in human blood and urine was devised. Thiosulfate was first alkylated with pentafluorobenzyl bromide, with L-ascorbic acid as the stabilizer and sodium chloride as the catalyst. The alkylated thiosulfate was oxidized with iodine to obtain bis(pentafluorobenzyl)disulfide, which was then subjected to gas chromatography and gas chromatography/mass spectrometry. Mass fragmentography was used to identify the disulfide, and gas chromatography with an electron capture detector was used for quantitative determination. The lower limit of detection was 0.003 μmol/mL.

Original languageEnglish
Pages (from-to)148-150
Number of pages3
JournalJournal of Analytical Toxicology
Volume15
Issue number3
DOIs
Publication statusPublished - Jan 1 1991

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Chemical Health and Safety

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