Determination of total and pancreatic amylase activities in human blood by use of microchip electrophoresis

Eiki Maeda, Masatoshi Kataoka, Yasuo Shinohara, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

In this study, a high-performance determination system for isoenzyme activities of human a-amylase was developed using microchip electrophoresis (ME) with a plastic chip. The combination of ME for substrate/hydrolysate separation and immunomhibition method for differentiation of isoenzymes using anti human salivary amylase (S-AMY) monoclonal antibody, Tu88E8, realized the highly selective determination of amylase isoenzyme activities even in a complex matrix such as a blood sample. This paper demonstrates that this system enables us to determine amylase isoenzyme activities with easy operation and high accuracy, and it will be applicable to clinical diagnosis for acute pancreatic disorders.

Original languageEnglish
Title of host publicationProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
EditorsJean-Louis Viovy, Patrick Tabeling, Stephanie Descroix, Laurent Malaquin
PublisherChemical and Biological Microsystems Society
Pages65-67
Number of pages3
ISBN (Electronic)9780979806407
Publication statusPublished - 2007
Event11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 - Paris, France
Duration: Oct 7 2007Oct 11 2007

Publication series

NameProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007

Conference

Conference11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
CountryFrance
CityParis
Period10/7/0710/11/07

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Maeda, E., Kataoka, M., Shinohara, Y., Kaji, N., Tokeshi, M., & Baba, Y. (2007). Determination of total and pancreatic amylase activities in human blood by use of microchip electrophoresis. In J-L. Viovy, P. Tabeling, S. Descroix, & L. Malaquin (Eds.), Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 (pp. 65-67). (Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007). Chemical and Biological Microsystems Society.