Determination of Trace Amounts of Chiral Amino Acids in Complicated Biological Samples Using Two-Dimensional High-Performance Liquid Chromatography with an Innovative "Shape-Fitting" Peak Identification/Quantification Method

Kenji Hamase, Masashi Mita, Tatsuhiko Ikeda, Chiharu Ishii, Shoto Ishigo, Kei Masuyama, Takeyuki Akita, Aogu Furusho, Miho Takahashi, Tomomi Ide

Research output: Contribution to journalArticlepeer-review

Abstract

<p>A new peak identification/quantification method, <i>i.e.</i>, "shape-fitting" method, has been devised and applied to the determination of chiral amino acids in human plasma and urine. The shape fitting method enables the determination of target analytes using a part of the peak by mathematically predicting the whole peak even when the baseline is not clear. Using this method, D-glutamic acid (Glu) and D-proline (Pro) as well as their L-enantiomers in the plasma and urine were determined. The calibration lines of 4 target enantiomers were linear with correlation coefficients higher than 0.9998; the RSD values of the intra-day precision and inter-day precision were less than 6.70%. In the human plasma, trace amount of D-Pro (0.47 nmol/mL) was observed, and in the urine, 2.97 nmol/mL of D-Glu and 0.08 nmol/mL of D-Pro were successfully determined.</p><p></p>
Original languageEnglish
Pages (from-to)147-152
Number of pages6
JournalCHROMATOGRAPHY
Volume39
Issue number3
DOIs
Publication statusPublished - 2018

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