Determination of Trace Amounts of Chiral Amino Acids in Complicated Biological Samples Using Two-Dimensional High-Performance Liquid Chromatography with an Innovative "Shape-Fitting" Peak Identification/Quantification Method

Kenji Hamase; Masashi Mita; Tatsuhiko Ikeda; Chiharu Ishii; Shoto Ishigo; Kei Masuyama; Takeyuki Akita; Aogu Furusho; Miho Takahashi; Tomomi Ide

doi:10.15583/jpchrom.2018.019

Determination of Trace Amounts of Chiral Amino Acids in Complicated Biological Samples Using Two-Dimensional High-Performance Liquid Chromatography with an Innovative "Shape-Fitting" Peak Identification/Quantification Method

Kenji Hamase, Masashi Mita, Tatsuhiko Ikeda, Chiharu Ishii, Shoto Ishigo, Kei Masuyama, Takeyuki Akita, Aogu Furusho, Miho Takahashi, Tomomi Ide

Research output: Contribution to journal › Article › peer-review

Abstract

A new peak identification/quantification method, i.e., "shape-fitting" method, has been devised and applied to the determination of chiral amino acids in human plasma and urine. The shape fitting method enables the determination of target analytes using a part of the peak by mathematically predicting the whole peak even when the baseline is not clear. Using this method, D-glutamic acid (Glu) and D-proline (Pro) as well as their L-enantiomers in the plasma and urine were determined. The calibration lines of 4 target enantiomers were linear with correlation coefficients higher than 0.9998; the RSD values of the intra-day precision and inter-day precision were less than 6.70%. In the human plasma, trace amount of D-Pro (0.47 nmol/mL) was observed, and in the urine, 2.97 nmol/mL of D-Glu and 0.08 nmol/mL of D-Pro were successfully determined.

Original language	English
Pages (from-to)	147-152
Number of pages	6
Journal	CHROMATOGRAPHY
Volume	39
Issue number	3
DOIs	https://doi.org/10.15583/jpchrom.2018.019
Publication status	Published - 2018

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Hamase, K., Mita, M., Ikeda, T., Ishii, C., Ishigo, S., Masuyama, K., Akita, T., Furusho, A., Takahashi, M., & Ide, T. (2018). Determination of Trace Amounts of Chiral Amino Acids in Complicated Biological Samples Using Two-Dimensional High-Performance Liquid Chromatography with an Innovative "Shape-Fitting" Peak Identification/Quantification Method. CHROMATOGRAPHY, 39(3), 147-152. https://doi.org/10.15583/jpchrom.2018.019

Determination of Trace Amounts of Chiral Amino Acids in Complicated Biological Samples Using Two-Dimensional High-Performance Liquid Chromatography with an Innovative "Shape-Fitting" Peak Identification/Quantification Method. / Hamase, Kenji; Mita, Masashi; Ikeda, Tatsuhiko et al.

In: CHROMATOGRAPHY, Vol. 39, No. 3, 2018, p. 147-152.

Research output: Contribution to journal › Article › peer-review

Hamase, K, Mita, M, Ikeda, T, Ishii, C, Ishigo, S, Masuyama, K, Akita, T, Furusho, A, Takahashi, M & Ide, T 2018, 'Determination of Trace Amounts of Chiral Amino Acids in Complicated Biological Samples Using Two-Dimensional High-Performance Liquid Chromatography with an Innovative "Shape-Fitting" Peak Identification/Quantification Method', CHROMATOGRAPHY, vol. 39, no. 3, pp. 147-152. https://doi.org/10.15583/jpchrom.2018.019

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abstract = "A new peak identification/quantification method, i.e., {"}shape-fitting{"} method, has been devised and applied to the determination of chiral amino acids in human plasma and urine. The shape fitting method enables the determination of target analytes using a part of the peak by mathematically predicting the whole peak even when the baseline is not clear. Using this method, D-glutamic acid (Glu) and D-proline (Pro) as well as their L-enantiomers in the plasma and urine were determined. The calibration lines of 4 target enantiomers were linear with correlation coefficients higher than 0.9998; the RSD values of the intra-day precision and inter-day precision were less than 6.70%. In the human plasma, trace amount of D-Pro (0.47 nmol/mL) was observed, and in the urine, 2.97 nmol/mL of D-Glu and 0.08 nmol/mL of D-Pro were successfully determined.",

author = "Kenji Hamase and Masashi Mita and Tatsuhiko Ikeda and Chiharu Ishii and Shoto Ishigo and Kei Masuyama and Takeyuki Akita and Aogu Furusho and Miho Takahashi and Tomomi Ide",

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AU - Mita, Masashi

AU - Ikeda, Tatsuhiko

AU - Ishii, Chiharu

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AU - Masuyama, Kei

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AU - Furusho, Aogu

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AB - A new peak identification/quantification method, i.e., "shape-fitting" method, has been devised and applied to the determination of chiral amino acids in human plasma and urine. The shape fitting method enables the determination of target analytes using a part of the peak by mathematically predicting the whole peak even when the baseline is not clear. Using this method, D-glutamic acid (Glu) and D-proline (Pro) as well as their L-enantiomers in the plasma and urine were determined. The calibration lines of 4 target enantiomers were linear with correlation coefficients higher than 0.9998; the RSD values of the intra-day precision and inter-day precision were less than 6.70%. In the human plasma, trace amount of D-Pro (0.47 nmol/mL) was observed, and in the urine, 2.97 nmol/mL of D-Glu and 0.08 nmol/mL of D-Pro were successfully determined.

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