Determining the activation of rho as an index of receptor coupling to G12/13 proteins

Research output: Contribution to journalArticle

Abstract

Heterotrimeric G proteins are composed of α, β, and γ subunits. G proteins can be activated by a large number of cell-surface hepathelical receptors and can transduce signals from these receptors to various intracellular signaling molecules. When G protein-coupled receptors are bound by their cognate ligand, interaction with specific subtypes of G protein leads to dissociation of the α subunit of the heterotrimeric G protein from the βγ dimer, and both Gα-GTP and Gβγ are capable of initiating their own signal transduction pathways. G proteins are functionally divided into four groups based on the nature of α subunit into G s, Gi, Gq, and G12 families. The members of the G12 subfamily are G12 and G13. Increasing evidence indicates that G12/13 proteins play critical roles in various physiological functions. G12 and G13 regulate the small GTPase Rho through modulation of guanine nucleotide exchange factor (RhoGEF) activity to regulate various cellular responses, such as cytoskeletal changes and cell growth. Therefore, Rho activity can often represent a sensitive marker of G12/13 activity. Here, we describe the Rho activation assay to monitor the activity of G12/13 proteins.

Original languageEnglish
Pages (from-to)317-327
Number of pages11
JournalMethods in Molecular Biology
Volume746
DOIs
Publication statusPublished - Jan 1 2011

Fingerprint

G12-G13 GTP-Binding Protein alpha Subunits
GTP-Binding Proteins
Heterotrimeric GTP-Binding Proteins
Rho Guanine Nucleotide Exchange Factors
Guanine Nucleotide Exchange Factors
Monomeric GTP-Binding Proteins
Protein Subunits
Cell Surface Receptors
G-Protein-Coupled Receptors
Guanosine Triphosphate
Signal Transduction
Ligands
Growth

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

@article{ede1328f527142789fbe3c706221f9dd,
title = "Determining the activation of rho as an index of receptor coupling to G12/13 proteins",
abstract = "Heterotrimeric G proteins are composed of α, β, and γ subunits. G proteins can be activated by a large number of cell-surface hepathelical receptors and can transduce signals from these receptors to various intracellular signaling molecules. When G protein-coupled receptors are bound by their cognate ligand, interaction with specific subtypes of G protein leads to dissociation of the α subunit of the heterotrimeric G protein from the βγ dimer, and both Gα-GTP and Gβγ are capable of initiating their own signal transduction pathways. G proteins are functionally divided into four groups based on the nature of α subunit into G s, Gi, Gq, and G12 families. The members of the G12 subfamily are G12 and G13. Increasing evidence indicates that G12/13 proteins play critical roles in various physiological functions. G12 and G13 regulate the small GTPase Rho through modulation of guanine nucleotide exchange factor (RhoGEF) activity to regulate various cellular responses, such as cytoskeletal changes and cell growth. Therefore, Rho activity can often represent a sensitive marker of G12/13 activity. Here, we describe the Rho activation assay to monitor the activity of G12/13 proteins.",
author = "Nakaya Michio and Mina Ohba and Motohiro Nishida and Hitoshi Kurose",
year = "2011",
month = "1",
day = "1",
doi = "10.1007/978-1-61779-126-0_17",
language = "English",
volume = "746",
pages = "317--327",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

TY - JOUR

T1 - Determining the activation of rho as an index of receptor coupling to G12/13 proteins

AU - Michio, Nakaya

AU - Ohba, Mina

AU - Nishida, Motohiro

AU - Kurose, Hitoshi

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Heterotrimeric G proteins are composed of α, β, and γ subunits. G proteins can be activated by a large number of cell-surface hepathelical receptors and can transduce signals from these receptors to various intracellular signaling molecules. When G protein-coupled receptors are bound by their cognate ligand, interaction with specific subtypes of G protein leads to dissociation of the α subunit of the heterotrimeric G protein from the βγ dimer, and both Gα-GTP and Gβγ are capable of initiating their own signal transduction pathways. G proteins are functionally divided into four groups based on the nature of α subunit into G s, Gi, Gq, and G12 families. The members of the G12 subfamily are G12 and G13. Increasing evidence indicates that G12/13 proteins play critical roles in various physiological functions. G12 and G13 regulate the small GTPase Rho through modulation of guanine nucleotide exchange factor (RhoGEF) activity to regulate various cellular responses, such as cytoskeletal changes and cell growth. Therefore, Rho activity can often represent a sensitive marker of G12/13 activity. Here, we describe the Rho activation assay to monitor the activity of G12/13 proteins.

AB - Heterotrimeric G proteins are composed of α, β, and γ subunits. G proteins can be activated by a large number of cell-surface hepathelical receptors and can transduce signals from these receptors to various intracellular signaling molecules. When G protein-coupled receptors are bound by their cognate ligand, interaction with specific subtypes of G protein leads to dissociation of the α subunit of the heterotrimeric G protein from the βγ dimer, and both Gα-GTP and Gβγ are capable of initiating their own signal transduction pathways. G proteins are functionally divided into four groups based on the nature of α subunit into G s, Gi, Gq, and G12 families. The members of the G12 subfamily are G12 and G13. Increasing evidence indicates that G12/13 proteins play critical roles in various physiological functions. G12 and G13 regulate the small GTPase Rho through modulation of guanine nucleotide exchange factor (RhoGEF) activity to regulate various cellular responses, such as cytoskeletal changes and cell growth. Therefore, Rho activity can often represent a sensitive marker of G12/13 activity. Here, we describe the Rho activation assay to monitor the activity of G12/13 proteins.

UR - http://www.scopus.com/inward/record.url?scp=80052833620&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052833620&partnerID=8YFLogxK

U2 - 10.1007/978-1-61779-126-0_17

DO - 10.1007/978-1-61779-126-0_17

M3 - Article

VL - 746

SP - 317

EP - 327

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -