Deuterium NMR of Raft Model Membranes Reveals Domain-Specific Order Profiles and Compositional Distribution

Tomokazu Yasuda, Hiroshi Tsuchikawa, Michio Murata, Nobuaki Matsumori

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

In this report, we applied site-specifically deuterated N-stearoylsphingomyelins (SSMs) to raft-exhibiting ternary mixtures containing SSM, 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), and cholesterol (Chol) and successfully acquired deuterium quadrupole coupling profiles of SSM from liquid-ordered (Lo) and liquid-disordered (Ld) domains. To our knowledge, this is the first report that shows detailed lipid chain dynamics separately and simultaneously obtained from coexisting Lo and Ld domains. We also found that the quadrupole profile of the Lo phase in the ternary system was almost identical to that in the SSM-Chol binary mixture, suggesting that the order profile of the binary system is essentially applicable to more complicated membrane systems in terms of the acyl chain order. We also demonstrated that 2H NMR spectroscopy, in combination with organic synthesis of deuterated components, could be used to reveal the accurate mole fractions of each component distributed in the Lo and Ld domains. As compared with the reported tie-line analysis of phase diagrams, the merit of our 2H NMR analysis is that the domain-specific compositional fractions are directly attainable without experimental complexity and ambiguity. The accurate compositional distributions as well as lipid order profiles in ternary mixtures are relevant to understanding the molecular mechanism of lipid raft formation.

Original languageEnglish
Pages (from-to)2502-2506
Number of pages5
JournalBiophysical Journal
Volume108
Issue number10
DOIs
Publication statusPublished - May 19 2015

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Deuterium
Membranes
Lipids
Cholesterol
Synthetic Chemistry Techniques
Magnetic Resonance Spectroscopy
N-stearoylsphingomyelin

All Science Journal Classification (ASJC) codes

  • Biophysics

Cite this

Deuterium NMR of Raft Model Membranes Reveals Domain-Specific Order Profiles and Compositional Distribution. / Yasuda, Tomokazu; Tsuchikawa, Hiroshi; Murata, Michio; Matsumori, Nobuaki.

In: Biophysical Journal, Vol. 108, No. 10, 19.05.2015, p. 2502-2506.

Research output: Contribution to journalArticle

Yasuda, Tomokazu ; Tsuchikawa, Hiroshi ; Murata, Michio ; Matsumori, Nobuaki. / Deuterium NMR of Raft Model Membranes Reveals Domain-Specific Order Profiles and Compositional Distribution. In: Biophysical Journal. 2015 ; Vol. 108, No. 10. pp. 2502-2506.
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