Development and characterization of a new Bombyx mori cell line for protein expression

Arun M. Khurad, Ravindra S. Bahekar, Min Juan Zhang, Ashish D. Tiple, Jae Man Lee, Chuan Xi Zhang, Takahiro Kusakabe

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

A Bombyx mori continuous cell line, designated DZNU-Bm-17, was established from larval ovaries. The cells were initially grown in MGM-448 insect cell culture medium supplemented with 10% fetal bovine serum and 3% heat inactivated B. mori hemolymph at 25 ± 1 °C and later adapted gradually to TNM-FH medium. Partially adhered refractive cells were the predominant cell type in the culture. The cells took about 1055. days to complete 100 passages in TNM-FH medium. The population doubling time of the cell line was about 30-34. h at 25 ± 1 °C. The cell population was largely diploid, but a few triploids and tetraploids were also observed. DNA profiles using simple sequence repeat loci established the differences between the DZNU-Bm-1, Bm-5, DZNU-Bm-12, DZNU-Bm-17, and BmN cell lines. The cell line was susceptible to budded virus of B. mori nucleopolyhedrovirus (BmNPV), and 85-92% of the cells harbored BmNPV with an average of 15 occlusion bodies/infected cell. The cells expressed the luciferase and green fluorescent proteins using the BmNPV bacmid vector. We suggest the usefulness of the DZNU-Bm-17 cell line for BmNPV-based baculoviral expression studies.

Original languageEnglish
Pages (from-to)17-22
Number of pages6
JournalJournal of Asia-Pacific Entomology
Volume16
Issue number1
DOIs
Publication statusPublished - Mar 1 2013

All Science Journal Classification (ASJC) codes

  • Insect Science

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