Development and characterization of a silica monolith immobilized enzyme micro-bioreactor

Koei Kawakami, Yoshihide Sera, Shinji Sakai, Tsutomu Ono, Hiroyuki Ijima

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Several 10-cm-long capillary tubes [made of poly(ether ether ketone) (PEEK)] with inside diameters of 0.1-2.0 mm were filled with silica monolith-immobilized protease derived by in situ sol-gel transition from a 1:4 mixture of tetramethoxysilane and methyltrimethoxysilane. Transesterification between 20 mM (S)-(-)-glycidol and 0.4 M vinyl n-butyrate in an organic solvent was used as the test reaction. The substrate solution flowed through the column at a flow rate of 0.0004-5.0 mL·min-1. The conversion in the micro-bioreactor was higher than that in the batch reactor at a high liquid flow rate. When three tubes were connected in series, the conversion at a fixed ratio of the mass of the enzyme to the liquid flow rate was increased by approximately 50%, because of the tripling of the flow rate as compared to the case with a single tube. Changes in the tube diameter had no influence on the conversion at a fixed superficial liquid velocity. Further, the conversion increased with a decrease in the enzyme content. These results were ascribed to the apparent effect of liquid-solid mass transfer and were analyzed quantitatively using a simple mathematical model.

Original languageEnglish
Pages (from-to)236-240
Number of pages5
JournalIndustrial and Engineering Chemistry Research
Volume44
Issue number1
Publication statusPublished - Jan 5 2005

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Immobilized Enzymes
Bioreactors
Silicon Dioxide
Enzymes
Silica
Flow rate
glycidol
Liquids
Capillary tubes
Polyether ether ketones
Butyrates
Transesterification
Batch reactors
Organic solvents
Sol-gels
Peptide Hydrolases
Mass transfer
Mathematical models
Substrates

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering

Cite this

Development and characterization of a silica monolith immobilized enzyme micro-bioreactor. / Kawakami, Koei; Sera, Yoshihide; Sakai, Shinji; Ono, Tsutomu; Ijima, Hiroyuki.

In: Industrial and Engineering Chemistry Research, Vol. 44, No. 1, 05.01.2005, p. 236-240.

Research output: Contribution to journalArticle

Kawakami, Koei ; Sera, Yoshihide ; Sakai, Shinji ; Ono, Tsutomu ; Ijima, Hiroyuki. / Development and characterization of a silica monolith immobilized enzyme micro-bioreactor. In: Industrial and Engineering Chemistry Research. 2005 ; Vol. 44, No. 1. pp. 236-240.
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