Development and characterization of hybrid tubular structure of PLCL porous scaffold with hMSCs/ECs cell sheet

Azizah Intan Pangesty, Takaaki Arahira, Mitsugu Todo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Abstract: Tissue engineering offers an alternate approach to providing vascular graft with potential to grow similar with native tissue by seeding autologous cells into biodegradable scaffold. In this study, we developed a combining technique by layering a sheet of cells onto a porous tubular scaffold. The cell sheet prepared from co-culturing human mesenchymal stem cells (hMSCs) and endothelial cells (ECs) were able to infiltrate through porous structure of the tubular poly (lactide-co-caprolactone) (PLCL) scaffold and further proliferated on luminal wall within a week of culture. Moreover, the co-culture cell sheet within the tubular scaffold has demonstrated a faster proliferation rate than the monoculture cell sheet composed of MSCs only. We also found that the co-culture cell sheet expressed a strong angiogenic marker, including vascular endothelial growth factor (VEGF) and its receptor (VEGFR), as compared with the monoculture cell sheet within 2 weeks of culture, indicating that the co-culture system could induce differentiation into endothelial cell lineage. This combined technique would provide cellularization and maturation of vascular construct in relatively short period with a strong expression of angiogenic properties. Graphical abstract: [InlineMediaObject not available: see fulltext.].

Original languageEnglish
Article number165
JournalJournal of Materials Science: Materials in Medicine
Volume28
Issue number10
DOIs
Publication statusPublished - Oct 1 2017

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Endothelial cells
Scaffolds (biology)
Stem cells
Mesenchymal Stromal Cells
Cell culture
Endothelial Cells
Scaffolds
Coculture Techniques
Vascular Endothelial Growth Factor Receptor
Blood Vessels
Tissue engineering
Grafts
Tissue
Cell Lineage
Tissue Engineering
caprolactone
poly(lactide)
Transplants

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Development and characterization of hybrid tubular structure of PLCL porous scaffold with hMSCs/ECs cell sheet. / Pangesty, Azizah Intan; Arahira, Takaaki; Todo, Mitsugu.

In: Journal of Materials Science: Materials in Medicine, Vol. 28, No. 10, 165, 01.10.2017.

Research output: Contribution to journalArticle

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