Development and characterization of simple sequence repeat DNA markers for Zelkova serrata

E. Fukatsu, K. Isoda, T. Hirao, M. Takahashi, Atsushi Watanabe

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We developed 14 microsatellite loci from an enriched genomic DNA library of a broad-leaved deciduous tree, Zelkova serrata. Of 198 clones from the library, 112 contained microsatellite repeat regions. The M13-tailed primer method was used for economy. Sequence-specific primer pairs were designed for 58 of 76 candidate clones. Fourteen of these primer pairs successfully amplified polymorphic single loci among 34 individuals collected from the Kanto breeding region in Japan. The expected heterozygosity for the 14 microsatellite markers ranged from 0.378 to 0.876, suggesting that these will prove valuable for breeding and ecological studies on Z. serrata.

Original languageEnglish
Pages (from-to)378-380
Number of pages3
JournalMolecular Ecology Notes
Volume5
Issue number2
DOIs
Publication statusPublished - Jun 1 2005
Externally publishedYes

Fingerprint

Ulmaceae
Zelkova serrata
Genetic Markers
Microsatellite Repeats
clone
breeding
microsatellite repeats
DNA
genetic markers
Breeding
deciduous tree
Clone Cells
heterozygosity
clones
genomics
loci
Genomic Library
DNA libraries
Gene Library
Libraries

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Development and characterization of simple sequence repeat DNA markers for Zelkova serrata. / Fukatsu, E.; Isoda, K.; Hirao, T.; Takahashi, M.; Watanabe, Atsushi.

In: Molecular Ecology Notes, Vol. 5, No. 2, 01.06.2005, p. 378-380.

Research output: Contribution to journalArticle

Fukatsu, E. ; Isoda, K. ; Hirao, T. ; Takahashi, M. ; Watanabe, Atsushi. / Development and characterization of simple sequence repeat DNA markers for Zelkova serrata. In: Molecular Ecology Notes. 2005 ; Vol. 5, No. 2. pp. 378-380.
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