Development and evaluation of rice giant embryo mutants for high oil content originated from a high-yielding cultivar ‘Mizuhochikara’

Mitsukazu Sakata, Mari Seno, Hiroaki Matsusaka, Kiyomi Takahashi, Yuki Nakamura, Yoshiyuki Yamagata, Enrique R. Angeles, Toshihiro Mochizuki, Toshihiro Kumamaru, Masao Sato, Akiko Enomoto, Kosuke Tashiro, Satoru Kuhara, Hikaru Satoh, Atsushi Yoshimura

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Rice bran oil is a byproduct of the milling of rice (Oryza sativa L.). It offers various health benefits and has a beneficial fatty acid composition. To increase the amount of rice bran as a sink for triacylglycerol (TAG), we developed and characterized new breeding materials with giant embryos. To induce mutants, we treated fertilized egg cells of the high-yielding cultivar ‘Mizuhochikara’ with N-methyl-N-nitrosourea (MNU). By screening M2 seeds, we isolated four giant embryo mutant lines. Genetic analysis revealed that the causative loci in lines MGE12 and MGE13 were allelic to giant embryo (ge) on chromosome 7, and had base changes in the causal gene Os07g0603700. On the other hand, the causative loci in lines MGE8 and MGE14 were not allelic to ge, and both were newly mapped on chromosome 3. The TAG contents of all four mutant lines increased relative to their wild type, ‘Mizuhochikara’. MGE13 was agronomically similar to ‘Mizuhochikara’ and would be useful for breeding for improved oil content.

Original languageEnglish
Pages (from-to)425-433
Number of pages9
JournalBreeding Science
Volume66
Issue number3
DOIs
Publication statusPublished - 2016

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Fingerprint Dive into the research topics of 'Development and evaluation of rice giant embryo mutants for high oil content originated from a high-yielding cultivar ‘Mizuhochikara’'. Together they form a unique fingerprint.

  • Cite this