Development of a combined technique using a rapid one-step immunochromatographic assay and indirect competitive ELISA for the rapid detection of baicalin

Madan Kumar Paudel, Waraporn Putalun, Boonchoo Sritularak, Osamu Morinaga, Yukihiro Shoyama, Hiroyuki Tanaka, Satoshi Morimoto

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A colloidal gold conjugated anti-baicalin monoclonal antibody (anti-BA MAb) was prepared and used in an immunochromatographic assay (ICA) for BA in Scutellariae Radix and Kampo medicines. This competitive ICA uses an anti-BA MAb which shows a high specificity for BA and baicalein. Its advantages include a short assay time (15min), no dependence on any instrumental systems, and it can detect BA in plant materials and Kampo medicines. The limit of detection for the ICA was found to be around 0.6μgmL -1of baicalin. Moreover, the usefulness of the combination of indirect competitive ELISA and the ICA using anti-BA MAb as a quality control method was confirmed for analysis of BA in Scutellariae Radix and Kampo medicines with a sufficient sensitivity (200ngmL -1 to 2μgmL -1), obtainable in an easy and timely manner.

Original languageEnglish
Pages (from-to)189-193
Number of pages5
JournalAnalytica Chimica Acta
Volume701
Issue number2
DOIs
Publication statusPublished - Sep 9 2011

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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