Development of a fluorescence peptide chip for the detection of caspase activity

Aishan Han, Tatsuhiko Sonoda, Jeong Hun Kang, Masaharu Murata, Takuro Niiidome, Yoshiki Katayama

    Research output: Contribution to journalArticlepeer-review

    7 Citations (Scopus)

    Abstract

    Proteases play a key role in cell functions, and it is very important to monitor their activities for drug screening and diagnosis of diseases. In the present study, a new class of fluorescence probe, into which a fluorophore and a quencher have been introduced, was developed and applied to the on-chip detection of caspase-3 activity. This probe is non fluorescent in the absence of caspase-3. However, when it is treated with active caspase-3, the fluorescence intensity increases dependent on the caspase-3 activity due to the cleavage of the quencher-containing moiety on a glass slide. This caspase-dependent increase in the fluorescence intensity was also detected when the glass slide immobilizing the probe peptide was treated with cell lysate stimulated by staurosporine (STP), which is an apoptosis-inducing agent. On the other hand, such an increase was not detected in the case of control cell lysate without STP-stimulation. The developed system is a rapid and sensitive method and is useful for the direct measurement of protease activity on a glass array.

    Original languageEnglish
    Pages (from-to)21-25
    Number of pages5
    JournalCombinatorial Chemistry and High Throughput Screening
    Volume9
    Issue number1
    DOIs
    Publication statusPublished - Jan 1 2006

    All Science Journal Classification (ASJC) codes

    • Drug Discovery
    • Computer Science Applications
    • Organic Chemistry

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