Abstract
A rare sugar is considered to be a monosaccharide rarely found in nature. To investigate their natural distribution and biological roles, a robust analytical system must be used to isolate, identify, and quantify them. Herein, we report the development of such a system that can specifically quantify and chromatographically separate four aldopentoses and eight aldohexoses tagged with 2-aminopyridine. Purified monosaccharides derivatized with a pyridylamino moiety (PA-monosaccharides) are first chromatographed over a high-performance anion-exchange resin. But, because two of the PA-aldohexoses used in this study, PA-talose and PA-idose, co-elute with the common saccharides, PA-glucose and PA-mannose, respectively, a second chromatographic step, reversed-phase high-performance liquid chromatography, is used to completely separate them. Thus, as shown by the results of this study, chromatographic separation of PA-monosaccharides is achievable and provides a quantitative measurement of common and rare isomeric aldopentoses and aldohexoses.
| Original language | English |
|---|---|
| Pages (from-to) | 5112-5115 |
| Number of pages | 4 |
| Journal | Journal of Chromatography A |
| Volume | 1216 |
| Issue number | 26 |
| DOIs | |
| Publication status | Published - Jun 26 2009 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Biochemistry
- Organic Chemistry
Cite this
Development of a high-sensitivity chromatographic separation system for pyridylaminated aldopentoses and aldohexoses. / Nakakita, Shin ichi; Hasehira, Kayo; Hosokawa, Tomohiro; Tokuda, Masaaki; Izumori, Ken; Takegawa, Kaoru; Hirabayashi, Jun.
In: Journal of Chromatography A, Vol. 1216, No. 26, 26.06.2009, p. 5112-5115.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of a high-sensitivity chromatographic separation system for pyridylaminated aldopentoses and aldohexoses
AU - Nakakita, Shin ichi
AU - Hasehira, Kayo
AU - Hosokawa, Tomohiro
AU - Tokuda, Masaaki
AU - Izumori, Ken
AU - Takegawa, Kaoru
AU - Hirabayashi, Jun
PY - 2009/6/26
Y1 - 2009/6/26
N2 - A rare sugar is considered to be a monosaccharide rarely found in nature. To investigate their natural distribution and biological roles, a robust analytical system must be used to isolate, identify, and quantify them. Herein, we report the development of such a system that can specifically quantify and chromatographically separate four aldopentoses and eight aldohexoses tagged with 2-aminopyridine. Purified monosaccharides derivatized with a pyridylamino moiety (PA-monosaccharides) are first chromatographed over a high-performance anion-exchange resin. But, because two of the PA-aldohexoses used in this study, PA-talose and PA-idose, co-elute with the common saccharides, PA-glucose and PA-mannose, respectively, a second chromatographic step, reversed-phase high-performance liquid chromatography, is used to completely separate them. Thus, as shown by the results of this study, chromatographic separation of PA-monosaccharides is achievable and provides a quantitative measurement of common and rare isomeric aldopentoses and aldohexoses.
AB - A rare sugar is considered to be a monosaccharide rarely found in nature. To investigate their natural distribution and biological roles, a robust analytical system must be used to isolate, identify, and quantify them. Herein, we report the development of such a system that can specifically quantify and chromatographically separate four aldopentoses and eight aldohexoses tagged with 2-aminopyridine. Purified monosaccharides derivatized with a pyridylamino moiety (PA-monosaccharides) are first chromatographed over a high-performance anion-exchange resin. But, because two of the PA-aldohexoses used in this study, PA-talose and PA-idose, co-elute with the common saccharides, PA-glucose and PA-mannose, respectively, a second chromatographic step, reversed-phase high-performance liquid chromatography, is used to completely separate them. Thus, as shown by the results of this study, chromatographic separation of PA-monosaccharides is achievable and provides a quantitative measurement of common and rare isomeric aldopentoses and aldohexoses.
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U2 - 10.1016/j.chroma.2009.04.072
DO - 10.1016/j.chroma.2009.04.072
M3 - Article
VL - 1216
SP - 5112
EP - 5115
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0021-9673
IS - 26
ER -