TY - JOUR
T1 - Development of a high-sensitivity chromatographic separation system for pyridylaminated aldopentoses and aldohexoses
AU - Nakakita, Shin ichi
AU - Hasehira, Kayo
AU - Hosokawa, Tomohiro
AU - Tokuda, Masaaki
AU - Izumori, Ken
AU - Takegawa, Kaoru
AU - Hirabayashi, Jun
PY - 2009/6/26
Y1 - 2009/6/26
N2 - A rare sugar is considered to be a monosaccharide rarely found in nature. To investigate their natural distribution and biological roles, a robust analytical system must be used to isolate, identify, and quantify them. Herein, we report the development of such a system that can specifically quantify and chromatographically separate four aldopentoses and eight aldohexoses tagged with 2-aminopyridine. Purified monosaccharides derivatized with a pyridylamino moiety (PA-monosaccharides) are first chromatographed over a high-performance anion-exchange resin. But, because two of the PA-aldohexoses used in this study, PA-talose and PA-idose, co-elute with the common saccharides, PA-glucose and PA-mannose, respectively, a second chromatographic step, reversed-phase high-performance liquid chromatography, is used to completely separate them. Thus, as shown by the results of this study, chromatographic separation of PA-monosaccharides is achievable and provides a quantitative measurement of common and rare isomeric aldopentoses and aldohexoses.
AB - A rare sugar is considered to be a monosaccharide rarely found in nature. To investigate their natural distribution and biological roles, a robust analytical system must be used to isolate, identify, and quantify them. Herein, we report the development of such a system that can specifically quantify and chromatographically separate four aldopentoses and eight aldohexoses tagged with 2-aminopyridine. Purified monosaccharides derivatized with a pyridylamino moiety (PA-monosaccharides) are first chromatographed over a high-performance anion-exchange resin. But, because two of the PA-aldohexoses used in this study, PA-talose and PA-idose, co-elute with the common saccharides, PA-glucose and PA-mannose, respectively, a second chromatographic step, reversed-phase high-performance liquid chromatography, is used to completely separate them. Thus, as shown by the results of this study, chromatographic separation of PA-monosaccharides is achievable and provides a quantitative measurement of common and rare isomeric aldopentoses and aldohexoses.
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U2 - 10.1016/j.chroma.2009.04.072
DO - 10.1016/j.chroma.2009.04.072
M3 - Article
C2 - 19467662
AN - SCOPUS:66049097309
VL - 1216
SP - 5112
EP - 5115
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0021-9673
IS - 26
ER -