Development of a high-sensitivity chromatographic separation system for pyridylaminated aldopentoses and aldohexoses

Shin ichi Nakakita, Kayo Hasehira, Tomohiro Hosokawa, Masaaki Tokuda, Ken Izumori, Kaoru Takegawa, Jun Hirabayashi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A rare sugar is considered to be a monosaccharide rarely found in nature. To investigate their natural distribution and biological roles, a robust analytical system must be used to isolate, identify, and quantify them. Herein, we report the development of such a system that can specifically quantify and chromatographically separate four aldopentoses and eight aldohexoses tagged with 2-aminopyridine. Purified monosaccharides derivatized with a pyridylamino moiety (PA-monosaccharides) are first chromatographed over a high-performance anion-exchange resin. But, because two of the PA-aldohexoses used in this study, PA-talose and PA-idose, co-elute with the common saccharides, PA-glucose and PA-mannose, respectively, a second chromatographic step, reversed-phase high-performance liquid chromatography, is used to completely separate them. Thus, as shown by the results of this study, chromatographic separation of PA-monosaccharides is achievable and provides a quantitative measurement of common and rare isomeric aldopentoses and aldohexoses.

Original languageEnglish
Pages (from-to)5112-5115
Number of pages4
JournalJournal of Chromatography A
Volume1216
Issue number26
DOIs
Publication statusPublished - Jun 26 2009

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Monosaccharides
Anion Exchange Resins
High performance liquid chromatography
Reverse-Phase Chromatography
Mannose
Sugars
High Pressure Liquid Chromatography
Glucose

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

Development of a high-sensitivity chromatographic separation system for pyridylaminated aldopentoses and aldohexoses. / Nakakita, Shin ichi; Hasehira, Kayo; Hosokawa, Tomohiro; Tokuda, Masaaki; Izumori, Ken; Takegawa, Kaoru; Hirabayashi, Jun.

In: Journal of Chromatography A, Vol. 1216, No. 26, 26.06.2009, p. 5112-5115.

Research output: Contribution to journalArticle

Nakakita, Shin ichi ; Hasehira, Kayo ; Hosokawa, Tomohiro ; Tokuda, Masaaki ; Izumori, Ken ; Takegawa, Kaoru ; Hirabayashi, Jun. / Development of a high-sensitivity chromatographic separation system for pyridylaminated aldopentoses and aldohexoses. In: Journal of Chromatography A. 2009 ; Vol. 1216, No. 26. pp. 5112-5115.
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AU - Izumori, Ken

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