TY - JOUR
T1 - Development of a hybrid artificial liver using a polyurethane foam/ hepatocyte-spheroid packed-bed module
AU - Ijima, H.
AU - Nakazawa, K.
AU - Koyama, S.
AU - Kaneko, M.
AU - Matsushita, T.
AU - Gion, T.
AU - Shirabe, K.
AU - Shimada, M.
AU - Takenaka, K.
AU - Sugimachi, K.
AU - Funatsu, K.
PY - 2000/6
Y1 - 2000/6
N2 - Primary dog hepatocytes spontaneously formed spheroids in the pores of polyurethane foam (PUF) within 1-2 days of stationary culture. The spheroids, about 100-150 μm in diameter, partly attached to the surface and immobilized inside these pores. The lidocaine disappearance rate decreased to about 4 μg/105 viable cells/day for 10 days, while in the PUF/spheroid culture the rate was maintained at almost the initial level of 8 μg/105 viable cells/day for 10 days. Then, two scales of PUF packed-bed modules were designed. A small module (PUF volume; 14.5 cm3) was used for in vitro culture to investigate optimum culture conditions, and a large module (PUF volume; 300 cm3) was designed for dog experiments. Hepatocytes inoculated in these modules also formed spheroids and maintained almost the same activity of albumin secretion rate (111 μg/cm3 PUF/day in the small module and 87.7 μg/cm3 PUF/day in the large module). These results indicate that the PUF packed-bed module containing hepatocyte-spheroids is promising as a hybrid artificial liver.
AB - Primary dog hepatocytes spontaneously formed spheroids in the pores of polyurethane foam (PUF) within 1-2 days of stationary culture. The spheroids, about 100-150 μm in diameter, partly attached to the surface and immobilized inside these pores. The lidocaine disappearance rate decreased to about 4 μg/105 viable cells/day for 10 days, while in the PUF/spheroid culture the rate was maintained at almost the initial level of 8 μg/105 viable cells/day for 10 days. Then, two scales of PUF packed-bed modules were designed. A small module (PUF volume; 14.5 cm3) was used for in vitro culture to investigate optimum culture conditions, and a large module (PUF volume; 300 cm3) was designed for dog experiments. Hepatocytes inoculated in these modules also formed spheroids and maintained almost the same activity of albumin secretion rate (111 μg/cm3 PUF/day in the small module and 87.7 μg/cm3 PUF/day in the large module). These results indicate that the PUF packed-bed module containing hepatocyte-spheroids is promising as a hybrid artificial liver.
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U2 - 10.1177/039139880002300607
DO - 10.1177/039139880002300607
M3 - Article
C2 - 10919756
AN - SCOPUS:0033940088
VL - 23
SP - 389
EP - 397
JO - Life Support Systems
JF - Life Support Systems
SN - 0391-3988
IS - 6
ER -