Development of a method for long-term preservation of Bombyx mori silkworm strains using frozen ovaries

Yutaka Banno, Kiyomi Nagasaki, Marino Tsukada, Yuko Minohara, Junko Banno, Kazuhiro Nishikawa, Kazunori Yamamoto, Kei Tamura, Tsuguru Fujii

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Abstract

Development of long-term preservation is essential for conservation of stocks of silkworm genetic resources. Thus far, a few methods have been reported, but more improvement is required for practical use. We have developed two effective modifications of a method for long-term preservation using frozen ovaries. One was slow cooling (1. °C per min) until -80. °C of the donor ovaries made possible by use of a BICELL freezing vessel. Using donor ovaries of 4th instar larvae, the average number of eggs laid per moth increased significantly from 110.7. ±. 53.4 eggs per moth by slow cooling with the BICELL vessel vs 12.3. ±. 10.3 eggs per moth by direct cooling in liquid nitrogen. A second improvement was connecting the thread bodies of the donor ovaries with those of the host in the transplantation step. Females operated on with the new method yielded a significantly higher percentage of moths that laid fertilized eggs than those transplanted with the standard procedure (70.4. ±. 21.6% vs 22.9. ±. 9.3%).

Original languageEnglish
Pages (from-to)283-287
Number of pages5
JournalCryobiology
Volume66
Issue number3
DOIs
Publication statusPublished - Jun 1 2013

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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    Banno, Y., Nagasaki, K., Tsukada, M., Minohara, Y., Banno, J., Nishikawa, K., Yamamoto, K., Tamura, K., & Fujii, T. (2013). Development of a method for long-term preservation of Bombyx mori silkworm strains using frozen ovaries. Cryobiology, 66(3), 283-287. https://doi.org/10.1016/j.cryobiol.2013.03.004