Development of a method for long-term preservation of Bombyx mori silkworm strains using frozen ovaries

Yutaka Banno, Kiyomi Nagasaki, Marino Tsukada, Yuko Minohara, Junko Banno, Kazuhiro Nishikawa, Kazunori Yamamoto, Kei Tamura, Tsuguru Fujii

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Development of long-term preservation is essential for conservation of stocks of silkworm genetic resources. Thus far, a few methods have been reported, but more improvement is required for practical use. We have developed two effective modifications of a method for long-term preservation using frozen ovaries. One was slow cooling (1. °C per min) until -80. °C of the donor ovaries made possible by use of a BICELL freezing vessel. Using donor ovaries of 4th instar larvae, the average number of eggs laid per moth increased significantly from 110.7. ±. 53.4 eggs per moth by slow cooling with the BICELL vessel vs 12.3. ±. 10.3 eggs per moth by direct cooling in liquid nitrogen. A second improvement was connecting the thread bodies of the donor ovaries with those of the host in the transplantation step. Females operated on with the new method yielded a significantly higher percentage of moths that laid fertilized eggs than those transplanted with the standard procedure (70.4. ±. 21.6% vs 22.9. ±. 9.3%).

Original languageEnglish
Pages (from-to)283-287
Number of pages5
JournalCryobiology
Volume66
Issue number3
DOIs
Publication statusPublished - Jun 1 2013

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Bombyx
Moths
Bombyx mori
silkworms
moths
Ovary
Eggs
Cooling
cooling
Liquid nitrogen
Freezing
Zygote
Conservation
methodology
genetic resources
Larva
freezing
Nitrogen
instars
Transplantation

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Development of a method for long-term preservation of Bombyx mori silkworm strains using frozen ovaries. / Banno, Yutaka; Nagasaki, Kiyomi; Tsukada, Marino; Minohara, Yuko; Banno, Junko; Nishikawa, Kazuhiro; Yamamoto, Kazunori; Tamura, Kei; Fujii, Tsuguru.

In: Cryobiology, Vol. 66, No. 3, 01.06.2013, p. 283-287.

Research output: Contribution to journalArticle

Banno, Y, Nagasaki, K, Tsukada, M, Minohara, Y, Banno, J, Nishikawa, K, Yamamoto, K, Tamura, K & Fujii, T 2013, 'Development of a method for long-term preservation of Bombyx mori silkworm strains using frozen ovaries', Cryobiology, vol. 66, no. 3, pp. 283-287. https://doi.org/10.1016/j.cryobiol.2013.03.004
Banno, Yutaka ; Nagasaki, Kiyomi ; Tsukada, Marino ; Minohara, Yuko ; Banno, Junko ; Nishikawa, Kazuhiro ; Yamamoto, Kazunori ; Tamura, Kei ; Fujii, Tsuguru. / Development of a method for long-term preservation of Bombyx mori silkworm strains using frozen ovaries. In: Cryobiology. 2013 ; Vol. 66, No. 3. pp. 283-287.
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