Development of a microdevice for facile analysis of theophylline in whole blood by a cloned enzyme donor immunoassay

Keine Nishiyama, Kanako Sugiura, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    We have developed a microdevice for therapeutic drug monitoring. In this device, dispensing of sample and reagent was accomplished by simple manual operation of a syringe. Moreover, for a simple and rapid measurement, we used cloned enzyme donor immunoassay as a detection principle. These features and the reagent that is enclosed in microdevice beforehand make it possible to complete the facile analysis. In this paper, our model analyte was 1,3-dimethylxanthine (theophylline), a kind of bronchodilator. The fluorescence measurement of theophylline in whole blood was achieved with the limit of detection of 0.73 μg mL−1. This microdevice provides rapid analysis (4 min), requires only a small volume of sample (2 μL) and features simple operation; hence, it is readily applicable to point of care testing.

    Original languageEnglish
    Pages (from-to)233-240
    Number of pages8
    JournalLab on a Chip
    Volume19
    Issue number2
    DOIs
    Publication statusPublished - Jan 21 2019

    All Science Journal Classification (ASJC) codes

    • Bioengineering
    • Biochemistry
    • Chemistry(all)
    • Biomedical Engineering

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