Abstract
We have developed a microdevice for therapeutic drug monitoring. In this device, dispensing of sample and reagent was accomplished by simple manual operation of a syringe. Moreover, for a simple and rapid measurement, we used cloned enzyme donor immunoassay as a detection principle. These features and the reagent that is enclosed in microdevice beforehand make it possible to complete the facile analysis. In this paper, our model analyte was 1,3-dimethylxanthine (theophylline), a kind of bronchodilator. The fluorescence measurement of theophylline in whole blood was achieved with the limit of detection of 0.73 μg mL −1 . This microdevice provides rapid analysis (4 min), requires only a small volume of sample (2 μL) and features simple operation; hence, it is readily applicable to point of care testing.
| Original language | English |
|---|---|
| Pages (from-to) | 233-240 |
| Number of pages | 8 |
| Journal | Lab on a Chip |
| Volume | 19 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jan 1 2019 |
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All Science Journal Classification (ASJC) codes
- Bioengineering
- Biochemistry
- Chemistry(all)
- Biomedical Engineering
Cite this
Development of a microdevice for facile analysis of theophylline in whole blood by a cloned enzyme donor immunoassay. / Nishiyama, Keine; Sugiura, Kanako; Kaji, Noritada; Tokeshi, Manabu; Baba, Yoshinobu.
In: Lab on a Chip, Vol. 19, No. 2, 01.01.2019, p. 233-240.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Development of a microdevice for facile analysis of theophylline in whole blood by a cloned enzyme donor immunoassay
AU - Nishiyama, Keine
AU - Sugiura, Kanako
AU - Kaji, Noritada
AU - Tokeshi, Manabu
AU - Baba, Yoshinobu
PY - 2019/1/1
Y1 - 2019/1/1
N2 - We have developed a microdevice for therapeutic drug monitoring. In this device, dispensing of sample and reagent was accomplished by simple manual operation of a syringe. Moreover, for a simple and rapid measurement, we used cloned enzyme donor immunoassay as a detection principle. These features and the reagent that is enclosed in microdevice beforehand make it possible to complete the facile analysis. In this paper, our model analyte was 1,3-dimethylxanthine (theophylline), a kind of bronchodilator. The fluorescence measurement of theophylline in whole blood was achieved with the limit of detection of 0.73 μg mL −1 . This microdevice provides rapid analysis (4 min), requires only a small volume of sample (2 μL) and features simple operation; hence, it is readily applicable to point of care testing.
AB - We have developed a microdevice for therapeutic drug monitoring. In this device, dispensing of sample and reagent was accomplished by simple manual operation of a syringe. Moreover, for a simple and rapid measurement, we used cloned enzyme donor immunoassay as a detection principle. These features and the reagent that is enclosed in microdevice beforehand make it possible to complete the facile analysis. In this paper, our model analyte was 1,3-dimethylxanthine (theophylline), a kind of bronchodilator. The fluorescence measurement of theophylline in whole blood was achieved with the limit of detection of 0.73 μg mL −1 . This microdevice provides rapid analysis (4 min), requires only a small volume of sample (2 μL) and features simple operation; hence, it is readily applicable to point of care testing.
UR - http://www.scopus.com/inward/record.url?scp=85060015023&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85060015023&partnerID=8YFLogxK
U2 - 10.1039/c8lc01105b
DO - 10.1039/c8lc01105b
M3 - Article
C2 - 30547178
AN - SCOPUS:85060015023
VL - 19
SP - 233
EP - 240
JO - Lab on a Chip - Miniaturisation for Chemistry and Biology
JF - Lab on a Chip - Miniaturisation for Chemistry and Biology
SN - 1473-0197
IS - 2
ER -