Development of a microdevice for facile analysis of theophylline in whole blood by a cloned enzyme donor immunoassay

Keine Nishiyama, Kanako Sugiura, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We have developed a microdevice for therapeutic drug monitoring. In this device, dispensing of sample and reagent was accomplished by simple manual operation of a syringe. Moreover, for a simple and rapid measurement, we used cloned enzyme donor immunoassay as a detection principle. These features and the reagent that is enclosed in microdevice beforehand make it possible to complete the facile analysis. In this paper, our model analyte was 1,3-dimethylxanthine (theophylline), a kind of bronchodilator. The fluorescence measurement of theophylline in whole blood was achieved with the limit of detection of 0.73 μg mL −1 . This microdevice provides rapid analysis (4 min), requires only a small volume of sample (2 μL) and features simple operation; hence, it is readily applicable to point of care testing.

Original languageEnglish
Pages (from-to)233-240
Number of pages8
JournalLab on a Chip
Volume19
Issue number2
DOIs
Publication statusPublished - Jan 1 2019

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Theophylline
Immunoenzyme Techniques
Blood
Enzymes
Syringes
Drug Monitoring
Fluorescence
Bronchodilator Agents
Limit of Detection
Monitoring
Testing
Equipment and Supplies
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Development of a microdevice for facile analysis of theophylline in whole blood by a cloned enzyme donor immunoassay. / Nishiyama, Keine; Sugiura, Kanako; Kaji, Noritada; Tokeshi, Manabu; Baba, Yoshinobu.

In: Lab on a Chip, Vol. 19, No. 2, 01.01.2019, p. 233-240.

Research output: Contribution to journalArticle

Nishiyama, Keine ; Sugiura, Kanako ; Kaji, Noritada ; Tokeshi, Manabu ; Baba, Yoshinobu. / Development of a microdevice for facile analysis of theophylline in whole blood by a cloned enzyme donor immunoassay. In: Lab on a Chip. 2019 ; Vol. 19, No. 2. pp. 233-240.
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