TY - JOUR
T1 - Development of a multiple-marker RT-PCR assay for detection of micrometastases of hepatocellular carcinoma
AU - Miyamoto, A.
AU - Fujiwara, Y.
AU - Sakon, M.
AU - Nagano, H.
AU - Sugita, Y.
AU - Kondo, M.
AU - Eguchi, H.
AU - Dono, K.
AU - Umeshita, K.
AU - Nakamori, S.
AU - Monden, M.
N1 - Funding Information:
Manuscript received September 23, 1999; revised manuscript received January 24, 2000; accepted January 24, 2000. From the Second Department of Surgery, Osaka University Medical School, Osaka, Japan. This work was supported by grants from the Ministry of Education, Culture, and Science of Japan. Address for reprint requests: Dr. Yoshiyuki Fujiwara, Department of Surgery II, Osaka University Medical School, 2-2 Yamada-Oka, Suita-City, Osaka 565-0871, Japan.
PY - 2000
Y1 - 2000
N2 - We investigated the expression of several candidate gene markers: MAGE-1, MAGE-3, cytokeratin-20 (CK-20), and α-fetoprotein (AFP) in tumor tissue and blood specimens from patients with hepatocellular carcinoma to develop a multiple marker reverse transcriptasepolymerase chain reaction (RT-PCR) assay for detection of micrometastasis in circulation. In 24 tumor specimens, the positivity for MAGE-1, MAGE-3, AFP, and CK-20 genes was 71, 67, 88, and 79% respectively, and all specimens expressed at least one marker. Although AFP and CK-20 transcripts were also detected in corresponding noncancerous liver specimens, none of the 22 corresponding normal specimens or seven normal livers were positive for MAGE-1 or MAGE-3 transcripts. In addition, MAGE-1 and MAGE-3 gene transcripts were not detected in any peripheral blood specimens from 31 normal healthy volunteers. MAGE-1, MAGE-3, and AFP transcripts were detected in 9 (12.7%), 3 (4.8%), and 10 (15.9%) of 71 blood specimens from 11 hepatocellular carcinoma patients, respectively, while 19 specimens (26.8%) were positive for at least one marker. Our results indicate that a multimarker RT-PCR assay with cancer-specific markers such as MAGE-1 and MAGE-3 in combination with a liver-specific AFP marker may be a promising diagnostic tool for monitoring hepatocellular carcinoma patients with better sensitivity and specificity.
AB - We investigated the expression of several candidate gene markers: MAGE-1, MAGE-3, cytokeratin-20 (CK-20), and α-fetoprotein (AFP) in tumor tissue and blood specimens from patients with hepatocellular carcinoma to develop a multiple marker reverse transcriptasepolymerase chain reaction (RT-PCR) assay for detection of micrometastasis in circulation. In 24 tumor specimens, the positivity for MAGE-1, MAGE-3, AFP, and CK-20 genes was 71, 67, 88, and 79% respectively, and all specimens expressed at least one marker. Although AFP and CK-20 transcripts were also detected in corresponding noncancerous liver specimens, none of the 22 corresponding normal specimens or seven normal livers were positive for MAGE-1 or MAGE-3 transcripts. In addition, MAGE-1 and MAGE-3 gene transcripts were not detected in any peripheral blood specimens from 31 normal healthy volunteers. MAGE-1, MAGE-3, and AFP transcripts were detected in 9 (12.7%), 3 (4.8%), and 10 (15.9%) of 71 blood specimens from 11 hepatocellular carcinoma patients, respectively, while 19 specimens (26.8%) were positive for at least one marker. Our results indicate that a multimarker RT-PCR assay with cancer-specific markers such as MAGE-1 and MAGE-3 in combination with a liver-specific AFP marker may be a promising diagnostic tool for monitoring hepatocellular carcinoma patients with better sensitivity and specificity.
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U2 - 10.1023/A:1005512221685
DO - 10.1023/A:1005512221685
M3 - Article
C2 - 10961717
AN - SCOPUS:0033870378
SN - 0163-2116
VL - 45
SP - 1376
EP - 1382
JO - Digestive Diseases and Sciences
JF - Digestive Diseases and Sciences
IS - 7
ER -
