Development of a new host vector system in mycobacteria

Yoshitaka Goto, Hatsumi Taniguchi, Takezo Udou, Yasuo Mizuguchi, Tohru Tokunaga

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The hybrid plasmid pYT72/pYT92 constructed from an Escherichia coli plasmid pACYC177 and mycobacterial plasmid pMSC262 isolated from Mycobacterium scroflaceum strain W262 transformed both E. coli and BCG. Phage-sensitive mutants S-10 and S-20 isolated from BCG Tokyo strain showed higher frequency of transformation than the wild-type strain. Frequency of transformation was dependent on age of the culture and the electroporation condition used. Several deletion mutants were generated from pYT72/92 to determine the minimum region for the replication in the mycobacteria. A 2.3-kb fragment of pMSC262 was found to contain an essential region. Using this fragment and pACYC177, a small shuttle vector pYT937 containing two drug-resistance markers, kanamycin- and ampicillin-resistance, was constructed. pYT937 contains AatII, BamHI, BbvII, GsuI, HincII, PstI, ScaI and XbaI cloning sites.

Original languageEnglish
Pages (from-to)277-282
Number of pages6
JournalFEMS microbiology letters
Volume83
Issue number3
DOIs
Publication statusPublished - Oct 15 1991
Externally publishedYes

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Mycobacterium
Plasmids
Mycobacterium bovis
S 10
Kanamycin Resistance
Escherichia coli
Ampicillin Resistance
Genetic Vectors
Electroporation
Tokyo
Drug Resistance
Bacteriophages
Organism Cloning

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Development of a new host vector system in mycobacteria. / Goto, Yoshitaka; Taniguchi, Hatsumi; Udou, Takezo; Mizuguchi, Yasuo; Tokunaga, Tohru.

In: FEMS microbiology letters, Vol. 83, No. 3, 15.10.1991, p. 277-282.

Research output: Contribution to journalArticle

Goto, Yoshitaka ; Taniguchi, Hatsumi ; Udou, Takezo ; Mizuguchi, Yasuo ; Tokunaga, Tohru. / Development of a new host vector system in mycobacteria. In: FEMS microbiology letters. 1991 ; Vol. 83, No. 3. pp. 277-282.
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