Development of a novel PCR method to comprehensively analyze salivary bacterial flora and its application to patients with odontogenic infections

Tomonori Akiyama, Hiroshi Miyamoto, Kazumasa Fukuda, Naoto Sano, Nanako Katagiri, Takeo Shobuike, Akiko Kukita, Yoshio Yamashita, Hatsumi Taniguchi, Masaaki Goto

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Objective: The objective of this study was to develop a novel polymerase chain reaction (PCR) method to comprehensively analyze salivary bacterial flora. Study design: The bacterial flora in the saliva of 10 healthy persons and 11 patients with odontogenic infections were examined using a DNA extraction method with a high level of cell destruction efficiency and a novel universal primer set to amplify approximately 580 bp of the 16S rDNA sequence. Results: Streptococcus (54.5%), Neisseria (14.7%), Actinomyces (8.4%), Gemella (4.1%), Granulicatella (3.8%), and Prevotella (1.4%) were dominant in a total of 1655 clones examined from the saliva of the healthy subjects. The dominant genera differed among the patients with odontogenic infections (a total of 823 clones) and were entirely different from those of the healthy subjects. Conclusion: This novel comprehensive salivary bacterial flora analysis method may be a useful supportive method to identify causative agents of odontogenic infections.

Original languageEnglish
Pages (from-to)669-676
Number of pages8
JournalOral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology
Volume109
Issue number5
DOIs
Publication statusPublished - May 1 2010
Externally publishedYes

Fingerprint

Polymerase Chain Reaction
Infection
Saliva
Healthy Volunteers
Gemella
Clone Cells
Prevotella
Neisseria
Actinomyces
Ribosomal DNA
Streptococcus
DNA

All Science Journal Classification (ASJC) codes

  • Surgery
  • Oral Surgery
  • Otorhinolaryngology
  • Dentistry(all)

Cite this

Development of a novel PCR method to comprehensively analyze salivary bacterial flora and its application to patients with odontogenic infections. / Akiyama, Tomonori; Miyamoto, Hiroshi; Fukuda, Kazumasa; Sano, Naoto; Katagiri, Nanako; Shobuike, Takeo; Kukita, Akiko; Yamashita, Yoshio; Taniguchi, Hatsumi; Goto, Masaaki.

In: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology, Vol. 109, No. 5, 01.05.2010, p. 669-676.

Research output: Contribution to journalArticle

Akiyama, Tomonori ; Miyamoto, Hiroshi ; Fukuda, Kazumasa ; Sano, Naoto ; Katagiri, Nanako ; Shobuike, Takeo ; Kukita, Akiko ; Yamashita, Yoshio ; Taniguchi, Hatsumi ; Goto, Masaaki. / Development of a novel PCR method to comprehensively analyze salivary bacterial flora and its application to patients with odontogenic infections. In: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology. 2010 ; Vol. 109, No. 5. pp. 669-676.
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