Abstract
Objective: The objective of this study was to develop a novel polymerase chain reaction (PCR) method to comprehensively analyze salivary bacterial flora. Study design: The bacterial flora in the saliva of 10 healthy persons and 11 patients with odontogenic infections were examined using a DNA extraction method with a high level of cell destruction efficiency and a novel universal primer set to amplify approximately 580 bp of the 16S rDNA sequence. Results: Streptococcus (54.5%), Neisseria (14.7%), Actinomyces (8.4%), Gemella (4.1%), Granulicatella (3.8%), and Prevotella (1.4%) were dominant in a total of 1655 clones examined from the saliva of the healthy subjects. The dominant genera differed among the patients with odontogenic infections (a total of 823 clones) and were entirely different from those of the healthy subjects. Conclusion: This novel comprehensive salivary bacterial flora analysis method may be a useful supportive method to identify causative agents of odontogenic infections.
| Original language | English |
|---|---|
| Pages (from-to) | 669-676 |
| Number of pages | 8 |
| Journal | Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology |
| Volume | 109 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - May 1 2010 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Surgery
- Oral Surgery
- Otorhinolaryngology
- Dentistry(all)
Cite this
Development of a novel PCR method to comprehensively analyze salivary bacterial flora and its application to patients with odontogenic infections. / Akiyama, Tomonori; Miyamoto, Hiroshi; Fukuda, Kazumasa; Sano, Naoto; Katagiri, Nanako; Shobuike, Takeo; Kukita, Akiko; Yamashita, Yoshio; Taniguchi, Hatsumi; Goto, Masaaki.
In: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology, Vol. 109, No. 5, 01.05.2010, p. 669-676.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of a novel PCR method to comprehensively analyze salivary bacterial flora and its application to patients with odontogenic infections
AU - Akiyama, Tomonori
AU - Miyamoto, Hiroshi
AU - Fukuda, Kazumasa
AU - Sano, Naoto
AU - Katagiri, Nanako
AU - Shobuike, Takeo
AU - Kukita, Akiko
AU - Yamashita, Yoshio
AU - Taniguchi, Hatsumi
AU - Goto, Masaaki
PY - 2010/5/1
Y1 - 2010/5/1
N2 - Objective: The objective of this study was to develop a novel polymerase chain reaction (PCR) method to comprehensively analyze salivary bacterial flora. Study design: The bacterial flora in the saliva of 10 healthy persons and 11 patients with odontogenic infections were examined using a DNA extraction method with a high level of cell destruction efficiency and a novel universal primer set to amplify approximately 580 bp of the 16S rDNA sequence. Results: Streptococcus (54.5%), Neisseria (14.7%), Actinomyces (8.4%), Gemella (4.1%), Granulicatella (3.8%), and Prevotella (1.4%) were dominant in a total of 1655 clones examined from the saliva of the healthy subjects. The dominant genera differed among the patients with odontogenic infections (a total of 823 clones) and were entirely different from those of the healthy subjects. Conclusion: This novel comprehensive salivary bacterial flora analysis method may be a useful supportive method to identify causative agents of odontogenic infections.
AB - Objective: The objective of this study was to develop a novel polymerase chain reaction (PCR) method to comprehensively analyze salivary bacterial flora. Study design: The bacterial flora in the saliva of 10 healthy persons and 11 patients with odontogenic infections were examined using a DNA extraction method with a high level of cell destruction efficiency and a novel universal primer set to amplify approximately 580 bp of the 16S rDNA sequence. Results: Streptococcus (54.5%), Neisseria (14.7%), Actinomyces (8.4%), Gemella (4.1%), Granulicatella (3.8%), and Prevotella (1.4%) were dominant in a total of 1655 clones examined from the saliva of the healthy subjects. The dominant genera differed among the patients with odontogenic infections (a total of 823 clones) and were entirely different from those of the healthy subjects. Conclusion: This novel comprehensive salivary bacterial flora analysis method may be a useful supportive method to identify causative agents of odontogenic infections.
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U2 - 10.1016/j.tripleo.2009.10.045
DO - 10.1016/j.tripleo.2009.10.045
M3 - Article
VL - 109
SP - 669
EP - 676
JO - Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
JF - Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
SN - 2212-4403
IS - 5
ER -