Abstract
We developed a novel strain of red sea bream Pagrus major that showed resistance to red sea bream iridoviral disease (RSIVD) using marker-assisted selection combined with DNA-based family selection and evaluated survival of its G 2 in a farm environment. The G 1 population was derived from a single RSIVD-resistant male (G 0 ). The G 1 progeny of the resistant male were identified by DNA parentage analysis using microsatellites. Marker-assisted selection using a quantitative trait locus linked to the RSIVD-resistant trait (Pma4_014) was conducted, and individuals inheriting the resistant allele of Pma4_014 (+) were selected. Two G 2 populations were produced in 2016 and 2017, and reared for approximately three months, during the summer, at an aquaculture facility. A normal production cohort was also reared at the same farm in 2016 and survival compared. Survival rates of the G 2 populations produced in 2016 and 2017 were 78.8% and 83.5%, respectively. The survival rate of the 2016 normal production cohort was 63.8%. The segregation pattern of the resistant allele showed 1 (+/+): 2 (+/−): 1 (−/−) in G 2 , and genotype frequency of Pma4_014 was calculated using approximately 200 individuals collected from 2016 and 2017 G 2 populations before and after the field trial. Numbers of each genotype were estimated based on the number of introduced and surviving fish. Individuals with the resistant allele, (+/+) and (+/−), showed over 80% of the estimated survival rates, but individuals without the resistant allele (−/−) showed lower survival (50%) in both years. This result strongly suggests that the resistant allele dominantly affects RSIVD resistance, and the newly developed G 2 population is useful for further propagation of RSIVD-resistant red sea bream fingerlings.
| Original language | English |
|---|---|
| Pages (from-to) | 188-192 |
| Number of pages | 5 |
| Journal | Aquaculture |
| Volume | 506 |
| DOIs | |
| Publication status | Published - May 15 2019 |
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All Science Journal Classification (ASJC) codes
- Aquatic Science
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Development of a novel RSIVD-resistant strain of red sea bream (Pagrus major) by marker-assisted selection combined with DNA-based family selection. / Sawayama, Eitaro; Kitamura, Shin Ichi; Nakayama, Kei; Ohta, Kohei; Okamoto, Hiroyuki; Ozaki, Akiyuki; Takagi, Motohiro.
In: Aquaculture, Vol. 506, 15.05.2019, p. 188-192.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Development of a novel RSIVD-resistant strain of red sea bream (Pagrus major) by marker-assisted selection combined with DNA-based family selection
AU - Sawayama, Eitaro
AU - Kitamura, Shin Ichi
AU - Nakayama, Kei
AU - Ohta, Kohei
AU - Okamoto, Hiroyuki
AU - Ozaki, Akiyuki
AU - Takagi, Motohiro
PY - 2019/5/15
Y1 - 2019/5/15
N2 - We developed a novel strain of red sea bream Pagrus major that showed resistance to red sea bream iridoviral disease (RSIVD) using marker-assisted selection combined with DNA-based family selection and evaluated survival of its G 2 in a farm environment. The G 1 population was derived from a single RSIVD-resistant male (G 0 ). The G 1 progeny of the resistant male were identified by DNA parentage analysis using microsatellites. Marker-assisted selection using a quantitative trait locus linked to the RSIVD-resistant trait (Pma4_014) was conducted, and individuals inheriting the resistant allele of Pma4_014 (+) were selected. Two G 2 populations were produced in 2016 and 2017, and reared for approximately three months, during the summer, at an aquaculture facility. A normal production cohort was also reared at the same farm in 2016 and survival compared. Survival rates of the G 2 populations produced in 2016 and 2017 were 78.8% and 83.5%, respectively. The survival rate of the 2016 normal production cohort was 63.8%. The segregation pattern of the resistant allele showed 1 (+/+): 2 (+/−): 1 (−/−) in G 2 , and genotype frequency of Pma4_014 was calculated using approximately 200 individuals collected from 2016 and 2017 G 2 populations before and after the field trial. Numbers of each genotype were estimated based on the number of introduced and surviving fish. Individuals with the resistant allele, (+/+) and (+/−), showed over 80% of the estimated survival rates, but individuals without the resistant allele (−/−) showed lower survival (50%) in both years. This result strongly suggests that the resistant allele dominantly affects RSIVD resistance, and the newly developed G 2 population is useful for further propagation of RSIVD-resistant red sea bream fingerlings.
AB - We developed a novel strain of red sea bream Pagrus major that showed resistance to red sea bream iridoviral disease (RSIVD) using marker-assisted selection combined with DNA-based family selection and evaluated survival of its G 2 in a farm environment. The G 1 population was derived from a single RSIVD-resistant male (G 0 ). The G 1 progeny of the resistant male were identified by DNA parentage analysis using microsatellites. Marker-assisted selection using a quantitative trait locus linked to the RSIVD-resistant trait (Pma4_014) was conducted, and individuals inheriting the resistant allele of Pma4_014 (+) were selected. Two G 2 populations were produced in 2016 and 2017, and reared for approximately three months, during the summer, at an aquaculture facility. A normal production cohort was also reared at the same farm in 2016 and survival compared. Survival rates of the G 2 populations produced in 2016 and 2017 were 78.8% and 83.5%, respectively. The survival rate of the 2016 normal production cohort was 63.8%. The segregation pattern of the resistant allele showed 1 (+/+): 2 (+/−): 1 (−/−) in G 2 , and genotype frequency of Pma4_014 was calculated using approximately 200 individuals collected from 2016 and 2017 G 2 populations before and after the field trial. Numbers of each genotype were estimated based on the number of introduced and surviving fish. Individuals with the resistant allele, (+/+) and (+/−), showed over 80% of the estimated survival rates, but individuals without the resistant allele (−/−) showed lower survival (50%) in both years. This result strongly suggests that the resistant allele dominantly affects RSIVD resistance, and the newly developed G 2 population is useful for further propagation of RSIVD-resistant red sea bream fingerlings.
UR - http://www.scopus.com/inward/record.url?scp=85063265422&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85063265422&partnerID=8YFLogxK
U2 - 10.1016/j.aquaculture.2019.03.039
DO - 10.1016/j.aquaculture.2019.03.039
M3 - Article
AN - SCOPUS:85063265422
VL - 506
SP - 188
EP - 192
JO - Aquaculture
JF - Aquaculture
SN - 0044-8486
ER -