Development of a novel RSIVD-resistant strain of red sea bream (Pagrus major) by marker-assisted selection combined with DNA-based family selection

Eitaro Sawayama, Shin Ichi Kitamura, Kei Nakayama, Kohei Ohta, Hiroyuki Okamoto, Akiyuki Ozaki, Motohiro Takagi

Research output: Contribution to journalArticle

Abstract

We developed a novel strain of red sea bream Pagrus major that showed resistance to red sea bream iridoviral disease (RSIVD) using marker-assisted selection combined with DNA-based family selection and evaluated survival of its G 2 in a farm environment. The G 1 population was derived from a single RSIVD-resistant male (G 0 ). The G 1 progeny of the resistant male were identified by DNA parentage analysis using microsatellites. Marker-assisted selection using a quantitative trait locus linked to the RSIVD-resistant trait (Pma4_014) was conducted, and individuals inheriting the resistant allele of Pma4_014 (+) were selected. Two G 2 populations were produced in 2016 and 2017, and reared for approximately three months, during the summer, at an aquaculture facility. A normal production cohort was also reared at the same farm in 2016 and survival compared. Survival rates of the G 2 populations produced in 2016 and 2017 were 78.8% and 83.5%, respectively. The survival rate of the 2016 normal production cohort was 63.8%. The segregation pattern of the resistant allele showed 1 (+/+): 2 (+/−): 1 (−/−) in G 2 , and genotype frequency of Pma4_014 was calculated using approximately 200 individuals collected from 2016 and 2017 G 2 populations before and after the field trial. Numbers of each genotype were estimated based on the number of introduced and surviving fish. Individuals with the resistant allele, (+/+) and (+/−), showed over 80% of the estimated survival rates, but individuals without the resistant allele (−/−) showed lower survival (50%) in both years. This result strongly suggests that the resistant allele dominantly affects RSIVD resistance, and the newly developed G 2 population is useful for further propagation of RSIVD-resistant red sea bream fingerlings.

Original languageEnglish
Pages (from-to)188-192
Number of pages5
JournalAquaculture
Volume506
DOIs
Publication statusPublished - May 15 2019

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Pagrus major
marker-assisted selection
DNA
allele
alleles
survival rate
genotype
farm
disease resistance
parentage
farms
sea
family
marker
fingerlings
aquaculture
quantitative trait loci
field experimentation
microsatellite repeats
summer

All Science Journal Classification (ASJC) codes

  • Aquatic Science

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Development of a novel RSIVD-resistant strain of red sea bream (Pagrus major) by marker-assisted selection combined with DNA-based family selection. / Sawayama, Eitaro; Kitamura, Shin Ichi; Nakayama, Kei; Ohta, Kohei; Okamoto, Hiroyuki; Ozaki, Akiyuki; Takagi, Motohiro.

In: Aquaculture, Vol. 506, 15.05.2019, p. 188-192.

Research output: Contribution to journalArticle

Sawayama, Eitaro ; Kitamura, Shin Ichi ; Nakayama, Kei ; Ohta, Kohei ; Okamoto, Hiroyuki ; Ozaki, Akiyuki ; Takagi, Motohiro. / Development of a novel RSIVD-resistant strain of red sea bream (Pagrus major) by marker-assisted selection combined with DNA-based family selection. In: Aquaculture. 2019 ; Vol. 506. pp. 188-192.
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abstract = "We developed a novel strain of red sea bream Pagrus major that showed resistance to red sea bream iridoviral disease (RSIVD) using marker-assisted selection combined with DNA-based family selection and evaluated survival of its G 2 in a farm environment. The G 1 population was derived from a single RSIVD-resistant male (G 0 ). The G 1 progeny of the resistant male were identified by DNA parentage analysis using microsatellites. Marker-assisted selection using a quantitative trait locus linked to the RSIVD-resistant trait (Pma4_014) was conducted, and individuals inheriting the resistant allele of Pma4_014 (+) were selected. Two G 2 populations were produced in 2016 and 2017, and reared for approximately three months, during the summer, at an aquaculture facility. A normal production cohort was also reared at the same farm in 2016 and survival compared. Survival rates of the G 2 populations produced in 2016 and 2017 were 78.8{\%} and 83.5{\%}, respectively. The survival rate of the 2016 normal production cohort was 63.8{\%}. The segregation pattern of the resistant allele showed 1 (+/+): 2 (+/−): 1 (−/−) in G 2 , and genotype frequency of Pma4_014 was calculated using approximately 200 individuals collected from 2016 and 2017 G 2 populations before and after the field trial. Numbers of each genotype were estimated based on the number of introduced and surviving fish. Individuals with the resistant allele, (+/+) and (+/−), showed over 80{\%} of the estimated survival rates, but individuals without the resistant allele (−/−) showed lower survival (50{\%}) in both years. This result strongly suggests that the resistant allele dominantly affects RSIVD resistance, and the newly developed G 2 population is useful for further propagation of RSIVD-resistant red sea bream fingerlings.",
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