TY - JOUR
T1 - Development of a novel RSIVD-resistant strain of red sea bream (Pagrus major) by marker-assisted selection combined with DNA-based family selection
AU - Sawayama, Eitaro
AU - Kitamura, Shin Ichi
AU - Nakayama, Kei
AU - Ohta, Kohei
AU - Okamoto, Hiroyuki
AU - Ozaki, Akiyuki
AU - Takagi, Motohiro
N1 - Funding Information:
We thank the staff of Marua Suisan Co., Ltd . for collecting the fish samples. This work was partly supported by a grant from the Small and Medium Enterprise Agency, Japan , Grant Number 2538110036 .
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/5/15
Y1 - 2019/5/15
N2 -
We developed a novel strain of red sea bream Pagrus major that showed resistance to red sea bream iridoviral disease (RSIVD) using marker-assisted selection combined with DNA-based family selection and evaluated survival of its G
2
in a farm environment. The G
1
population was derived from a single RSIVD-resistant male (G
0
). The G
1
progeny of the resistant male were identified by DNA parentage analysis using microsatellites. Marker-assisted selection using a quantitative trait locus linked to the RSIVD-resistant trait (Pma4_014) was conducted, and individuals inheriting the resistant allele of Pma4_014 (+) were selected. Two G
2
populations were produced in 2016 and 2017, and reared for approximately three months, during the summer, at an aquaculture facility. A normal production cohort was also reared at the same farm in 2016 and survival compared. Survival rates of the G
2
populations produced in 2016 and 2017 were 78.8% and 83.5%, respectively. The survival rate of the 2016 normal production cohort was 63.8%. The segregation pattern of the resistant allele showed 1 (+/+): 2 (+/−): 1 (−/−) in G
2
, and genotype frequency of Pma4_014 was calculated using approximately 200 individuals collected from 2016 and 2017 G
2
populations before and after the field trial. Numbers of each genotype were estimated based on the number of introduced and surviving fish. Individuals with the resistant allele, (+/+) and (+/−), showed over 80% of the estimated survival rates, but individuals without the resistant allele (−/−) showed lower survival (50%) in both years. This result strongly suggests that the resistant allele dominantly affects RSIVD resistance, and the newly developed G
2
population is useful for further propagation of RSIVD-resistant red sea bream fingerlings.
AB -
We developed a novel strain of red sea bream Pagrus major that showed resistance to red sea bream iridoviral disease (RSIVD) using marker-assisted selection combined with DNA-based family selection and evaluated survival of its G
2
in a farm environment. The G
1
population was derived from a single RSIVD-resistant male (G
0
). The G
1
progeny of the resistant male were identified by DNA parentage analysis using microsatellites. Marker-assisted selection using a quantitative trait locus linked to the RSIVD-resistant trait (Pma4_014) was conducted, and individuals inheriting the resistant allele of Pma4_014 (+) were selected. Two G
2
populations were produced in 2016 and 2017, and reared for approximately three months, during the summer, at an aquaculture facility. A normal production cohort was also reared at the same farm in 2016 and survival compared. Survival rates of the G
2
populations produced in 2016 and 2017 were 78.8% and 83.5%, respectively. The survival rate of the 2016 normal production cohort was 63.8%. The segregation pattern of the resistant allele showed 1 (+/+): 2 (+/−): 1 (−/−) in G
2
, and genotype frequency of Pma4_014 was calculated using approximately 200 individuals collected from 2016 and 2017 G
2
populations before and after the field trial. Numbers of each genotype were estimated based on the number of introduced and surviving fish. Individuals with the resistant allele, (+/+) and (+/−), showed over 80% of the estimated survival rates, but individuals without the resistant allele (−/−) showed lower survival (50%) in both years. This result strongly suggests that the resistant allele dominantly affects RSIVD resistance, and the newly developed G
2
population is useful for further propagation of RSIVD-resistant red sea bream fingerlings.
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U2 - 10.1016/j.aquaculture.2019.03.039
DO - 10.1016/j.aquaculture.2019.03.039
M3 - Article
AN - SCOPUS:85063265422
VL - 506
SP - 188
EP - 192
JO - Aquaculture
JF - Aquaculture
SN - 0044-8486
ER -