TY - JOUR
T1 - Development of a practical online supercritical fluid extraction–supercritical fluid chromatography/mass spectrometry system with an integrated split-flow method
AU - Sakai, Miho
AU - Hayakawa, Yoshihiro
AU - Funada, Yasuhiro
AU - Ando, Takashi
AU - Fukusaki, Eiichiro
AU - Bamba, Takeshi
N1 - Funding Information:
This work was partially supported by the Development of Systems and Technology for Advanced Measurement and Analysis Project of the Japan Science and Technology Agency (JST) [M.S., Y.F., T.A., T.B.]; the AMED-CREST Programs (1 8gm0910010h0203, JPMJCR1395, JP18gm0910013 ) from the Japan Agency for Medical Research and Development (AMED) [T.B.]; a grant from the ALCA Program of JST [T.B.]; a Grant-in-Aid for Scientific Research on Innovative Areas (17H06304) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT) [T.B.]; a Grant-in-Aid for Scientific Research (C) (26505007) from Japan Society for the Promotion of Science (JSPS) [T.B.]; a Grant-in-Aid for Scientific Research (B) (18H01800) from JSPS [T.B.]. This study will be included in a dissertation submitted by Miho Sakai to Osaka University in partial fulfillment of the requirement for her doctoral degree.
Funding Information:
This work was partially supported by the Development of Systems and Technology for Advanced Measurement and Analysis Project of the Japan Science and Technology Agency (JST) [M.S. Y.F. T.A. T.B.]; the AMED-CREST Programs (18gm0910010h0203, JPMJCR1395, JP18gm0910013) from the Japan?Agency?for?Medical?Research and?Development?(AMED) [T.B.]; a grant from the ALCA Program of JST [T.B.]; a Grant-in-Aid for Scientific Research on Innovative Areas (17H06304) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT) [T.B.]; a Grant-in-Aid for Scienti?c Research (C) (26505007) from Japan Society for the Promotion of Science (JSPS) [T.B.]; a Grant-in-Aid for Scienti?c Research (B) (18H01800) from JSPS [T.B.]. This study will be included in a dissertation submitted by Miho Sakai to Osaka University in partial fulfillment of the requirement for her doctoral degree.
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/5/10
Y1 - 2019/5/10
N2 - Herein, we describe a practical online supercritical fluid extraction-supercritical fluid chromatography/mass spectrometry (SFE-SFC/MS) system with an integrated split-flow method and a pre-column trap method that is well suited for the continuous extraction and separation of a wide range of compounds, including hydrophilic ones. Although an SFE-SFC system with a splitting method is already commercially available, in this study, we added some new features to this system: 1) a splitting method that further reduces the amount of extractant introduced into SFC, 2) a trap column, connected before the analytical column, with a different separation mechanism than the analytical column in the system with the splitting method, and 3) a system for calculating the recovery rate of SFE during online SFE-SFC/MS. In the above setup, part of the analyzed extract is introduced into the separation section at a higher split ratio owing to the make-up pump flow rate, thus reducing the distortion of the target analyte peak shape caused by the use of a strong extractant. Furthermore, the separation efficiency is improved by the use of an additional pre-column capable of interacting with compounds weakly retained on the analytical column. Finally, we show that equalization of the SFE and autosampler injection conditions allows evaluation of the recovery rate of SFE during online SFE-SFC/MS.
AB - Herein, we describe a practical online supercritical fluid extraction-supercritical fluid chromatography/mass spectrometry (SFE-SFC/MS) system with an integrated split-flow method and a pre-column trap method that is well suited for the continuous extraction and separation of a wide range of compounds, including hydrophilic ones. Although an SFE-SFC system with a splitting method is already commercially available, in this study, we added some new features to this system: 1) a splitting method that further reduces the amount of extractant introduced into SFC, 2) a trap column, connected before the analytical column, with a different separation mechanism than the analytical column in the system with the splitting method, and 3) a system for calculating the recovery rate of SFE during online SFE-SFC/MS. In the above setup, part of the analyzed extract is introduced into the separation section at a higher split ratio owing to the make-up pump flow rate, thus reducing the distortion of the target analyte peak shape caused by the use of a strong extractant. Furthermore, the separation efficiency is improved by the use of an additional pre-column capable of interacting with compounds weakly retained on the analytical column. Finally, we show that equalization of the SFE and autosampler injection conditions allows evaluation of the recovery rate of SFE during online SFE-SFC/MS.
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U2 - 10.1016/j.chroma.2019.01.044
DO - 10.1016/j.chroma.2019.01.044
M3 - Article
C2 - 30712818
AN - SCOPUS:85060762811
VL - 1592
SP - 161
EP - 172
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
ER -