Development of a practical online supercritical fluid extraction–supercritical fluid chromatography/mass spectrometry system with an integrated split-flow method

Miho Sakai, Yoshihiro Hayakawa, Yasuhiro Funada, Takashi Ando, Eiichiro Fukusaki, Takeshi Bamba

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Herein, we describe a practical online supercritical fluid extraction-supercritical fluid chromatography/mass spectrometry (SFE-SFC/MS) system with an integrated split-flow method and a pre-column trap method that is well suited for the continuous extraction and separation of a wide range of compounds, including hydrophilic ones. Although an SFE-SFC system with a splitting method is already commercially available, in this study, we added some new features to this system: 1) a splitting method that further reduces the amount of extractant introduced into SFC, 2) a trap column, connected before the analytical column, with a different separation mechanism than the analytical column in the system with the splitting method, and 3) a system for calculating the recovery rate of SFE during online SFE-SFC/MS. In the above setup, part of the analyzed extract is introduced into the separation section at a higher split ratio owing to the make-up pump flow rate, thus reducing the distortion of the target analyte peak shape caused by the use of a strong extractant. Furthermore, the separation efficiency is improved by the use of an additional pre-column capable of interacting with compounds weakly retained on the analytical column. Finally, we show that equalization of the SFE and autosampler injection conditions allows evaluation of the recovery rate of SFE during online SFE-SFC/MS.

Original languageEnglish
Pages (from-to)161-172
Number of pages12
JournalJournal of Chromatography A
Volume1592
DOIs
Publication statusPublished - May 10 2019

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Supercritical Fluid Chromatography
Supercritical fluids
Chromatography
Mass spectrometry
Mass Spectrometry
Fluids
Recovery
Flow rate
Pumps
Injections

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

Development of a practical online supercritical fluid extraction–supercritical fluid chromatography/mass spectrometry system with an integrated split-flow method. / Sakai, Miho; Hayakawa, Yoshihiro; Funada, Yasuhiro; Ando, Takashi; Fukusaki, Eiichiro; Bamba, Takeshi.

In: Journal of Chromatography A, Vol. 1592, 10.05.2019, p. 161-172.

Research output: Contribution to journalArticle

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