Development of a practical small-scale circulation bioreactor and application to a drug metabolism simulator

Hiroyuki Ijima, Yasuo Kakeya, Takahiro Ogata, Takanori Sakai

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We developed an easy-to-use, small-scale circulation-type bioreactor system that enables the simultaneous evaluation of many specimens. Medium flow was generated by a magnetic stirrer in this system. Primary rat hepatocytes formed a monolayer, and there were no morphological differences between cells in circulation and stationary cultures. The mitochondrial activity of hepatocytes in the circulation culture was 23% lower than that in the stationary culture after 2 days of culture. On the other hand, albumin production activity in the circulation culture after 2 days of culture was 1.4 times higher than that in the stationary culture. Albumin production activity per cell in the circulation culture was 1.9 times higher than that in the stationary culture after 2 days of culture. In addition, lidocaine metabolism rate per cell in the circulation culture was 1.3 times higher than that in the stationary culture. The lidocaine clearance of the circulation culture in our circulation-type bioreactor was 1.3 times higher than that of the stationary culture. It was shown that this bioreactor is suitable for the expression of the liver-specific functions of primary rat hepatocytes. Therefore, we can expect that this circulation-type bioreactor system will be a practical drug metabolism simulator. Crown

Original languageEnglish
Pages (from-to)292-296
Number of pages5
JournalBiochemical Engineering Journal
Volume44
Issue number2-3
DOIs
Publication statusPublished - May 15 2009

Fingerprint

Bioreactors
Metabolism
Simulators
Hepatocytes
Lidocaine
Pharmaceutical Preparations
Rats
Albumins
Crowns
Liver
Monolayers

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Environmental Engineering
  • Biomedical Engineering

Cite this

Development of a practical small-scale circulation bioreactor and application to a drug metabolism simulator. / Ijima, Hiroyuki; Kakeya, Yasuo; Ogata, Takahiro; Sakai, Takanori.

In: Biochemical Engineering Journal, Vol. 44, No. 2-3, 15.05.2009, p. 292-296.

Research output: Contribution to journalArticle

@article{852b6144e46246fdbe1d0707b69c2384,
title = "Development of a practical small-scale circulation bioreactor and application to a drug metabolism simulator",
abstract = "We developed an easy-to-use, small-scale circulation-type bioreactor system that enables the simultaneous evaluation of many specimens. Medium flow was generated by a magnetic stirrer in this system. Primary rat hepatocytes formed a monolayer, and there were no morphological differences between cells in circulation and stationary cultures. The mitochondrial activity of hepatocytes in the circulation culture was 23{\%} lower than that in the stationary culture after 2 days of culture. On the other hand, albumin production activity in the circulation culture after 2 days of culture was 1.4 times higher than that in the stationary culture. Albumin production activity per cell in the circulation culture was 1.9 times higher than that in the stationary culture after 2 days of culture. In addition, lidocaine metabolism rate per cell in the circulation culture was 1.3 times higher than that in the stationary culture. The lidocaine clearance of the circulation culture in our circulation-type bioreactor was 1.3 times higher than that of the stationary culture. It was shown that this bioreactor is suitable for the expression of the liver-specific functions of primary rat hepatocytes. Therefore, we can expect that this circulation-type bioreactor system will be a practical drug metabolism simulator. Crown",
author = "Hiroyuki Ijima and Yasuo Kakeya and Takahiro Ogata and Takanori Sakai",
year = "2009",
month = "5",
day = "15",
doi = "10.1016/j.bej.2008.12.015",
language = "English",
volume = "44",
pages = "292--296",
journal = "Biochemical Engineering Journal",
issn = "1369-703X",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - Development of a practical small-scale circulation bioreactor and application to a drug metabolism simulator

AU - Ijima, Hiroyuki

AU - Kakeya, Yasuo

AU - Ogata, Takahiro

AU - Sakai, Takanori

PY - 2009/5/15

Y1 - 2009/5/15

N2 - We developed an easy-to-use, small-scale circulation-type bioreactor system that enables the simultaneous evaluation of many specimens. Medium flow was generated by a magnetic stirrer in this system. Primary rat hepatocytes formed a monolayer, and there were no morphological differences between cells in circulation and stationary cultures. The mitochondrial activity of hepatocytes in the circulation culture was 23% lower than that in the stationary culture after 2 days of culture. On the other hand, albumin production activity in the circulation culture after 2 days of culture was 1.4 times higher than that in the stationary culture. Albumin production activity per cell in the circulation culture was 1.9 times higher than that in the stationary culture after 2 days of culture. In addition, lidocaine metabolism rate per cell in the circulation culture was 1.3 times higher than that in the stationary culture. The lidocaine clearance of the circulation culture in our circulation-type bioreactor was 1.3 times higher than that of the stationary culture. It was shown that this bioreactor is suitable for the expression of the liver-specific functions of primary rat hepatocytes. Therefore, we can expect that this circulation-type bioreactor system will be a practical drug metabolism simulator. Crown

AB - We developed an easy-to-use, small-scale circulation-type bioreactor system that enables the simultaneous evaluation of many specimens. Medium flow was generated by a magnetic stirrer in this system. Primary rat hepatocytes formed a monolayer, and there were no morphological differences between cells in circulation and stationary cultures. The mitochondrial activity of hepatocytes in the circulation culture was 23% lower than that in the stationary culture after 2 days of culture. On the other hand, albumin production activity in the circulation culture after 2 days of culture was 1.4 times higher than that in the stationary culture. Albumin production activity per cell in the circulation culture was 1.9 times higher than that in the stationary culture after 2 days of culture. In addition, lidocaine metabolism rate per cell in the circulation culture was 1.3 times higher than that in the stationary culture. The lidocaine clearance of the circulation culture in our circulation-type bioreactor was 1.3 times higher than that of the stationary culture. It was shown that this bioreactor is suitable for the expression of the liver-specific functions of primary rat hepatocytes. Therefore, we can expect that this circulation-type bioreactor system will be a practical drug metabolism simulator. Crown

UR - http://www.scopus.com/inward/record.url?scp=61449203717&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=61449203717&partnerID=8YFLogxK

U2 - 10.1016/j.bej.2008.12.015

DO - 10.1016/j.bej.2008.12.015

M3 - Article

VL - 44

SP - 292

EP - 296

JO - Biochemical Engineering Journal

JF - Biochemical Engineering Journal

SN - 1369-703X

IS - 2-3

ER -