Development of a Rapid Immunochromatographic Strip Test for the Detection of Mulberroside A

Chadathorn Inyai, Jukrapun Komaikul, Tharita Kitisripanya, Hiroyuki Tanaka, Boonchoo Sritularak, Waraporn Putalun

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Introduction Mulberroside A (MuA) is the major active anti-tyrosinase compound in the root bark extract of Morus alba L. (Moraceae). Typically, MuA is widely employed as an active ingredient in whitening cosmetics. A rapid and simple assay system utilizing a small quantity of test sample is essential for the detection of MuA in large number of samples. An immunoassay using highly specific MuA polyclonal antibodies may be useful for the determination of small quantities of MuA in test samples. Objective To establish a rapid qualitative MuA test, an immunochromatographic strip test was developed using anti-MuA polyclonal antibodies (anti-MuA PAb). Methodology The qualitative assay was based on a competitive immunoassay where the detection reagent consisted of anti-MuA PAb colored with colloidal gold particles. The capture reagent was a MuA-ovalbumin (MuA-OVA) conjugate immobilized on the test strip membrane. Results A sample containing MuA and the detection reagent were incubated together with immobilized capture reagent on a nitrocellulose membrane. When MuA was present, it competed with the immobilized conjugates on the strip membrane to bind a limited amount of colored antibodies; thus, a positive sample showed no color on the capture spot zone. The detection limit for the strip test was 2 μg/mL. The developed immunochromatographic strip test was utilized to determine MuA in plants, medical preparations and cosmetic samples. Conclusion This immunochromatographic strip test is advantageous as a rapid, simple and sensitive screening method for the detection of MuA in plant extracts, cosmetic samples and pharmaceutical products. Mulberroside A (MuA) is the major active anti-tyrosinase compound in the root bark extract of Morus alba L. (Moraceae). Typically, MuA is widely employed as an active ingredient in whitening cosmetics. To establish a rapid qualitative MuA test, an immunochromatographic strip test was developed using anti-MuA polyclonal antibodies (anti-MuA PAb) colored with colloidal gold particles as the detector reagent. The capture reagent was a MuA-ovalbumin (MuA-OVA) conjugate immobilized on the test strip membrane. When MuA was present, it competed with the immobilized conjugates on the strip membrane to bind a limited amount of colored antibodies; thus, a positive sample showed no color on the capture spot zone. The detection limit for the strip test was 2 μg/ml. This immunochromatographic strip test is advantageous as a rapid, simple and sensitive screening method for the detection of MuA in plant extracts, cosmetic samples and pharmaceutical products.

Original languageEnglish
Pages (from-to)423-427
Number of pages5
JournalPhytochemical Analysis
Volume26
Issue number6
DOIs
Publication statusPublished - Nov 1 2015

Fingerprint

immunoaffinity chromatography
cosmetics
polyclonal antibodies
testing
sampling
Cosmetics
Morus alba
Moraceae
ovalbumin
active ingredients
immunoassays
plant extracts
gold
bark
detection limit
Antibodies
Membranes
screening
mulberroside A
drugs

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Food Science
  • Biochemistry
  • Molecular Medicine
  • Plant Science
  • Drug Discovery
  • Complementary and alternative medicine

Cite this

Inyai, C., Komaikul, J., Kitisripanya, T., Tanaka, H., Sritularak, B., & Putalun, W. (2015). Development of a Rapid Immunochromatographic Strip Test for the Detection of Mulberroside A. Phytochemical Analysis, 26(6), 423-427. https://doi.org/10.1002/pca.2576

Development of a Rapid Immunochromatographic Strip Test for the Detection of Mulberroside A. / Inyai, Chadathorn; Komaikul, Jukrapun; Kitisripanya, Tharita; Tanaka, Hiroyuki; Sritularak, Boonchoo; Putalun, Waraporn.

In: Phytochemical Analysis, Vol. 26, No. 6, 01.11.2015, p. 423-427.

Research output: Contribution to journalArticle

Inyai, C, Komaikul, J, Kitisripanya, T, Tanaka, H, Sritularak, B & Putalun, W 2015, 'Development of a Rapid Immunochromatographic Strip Test for the Detection of Mulberroside A', Phytochemical Analysis, vol. 26, no. 6, pp. 423-427. https://doi.org/10.1002/pca.2576
Inyai C, Komaikul J, Kitisripanya T, Tanaka H, Sritularak B, Putalun W. Development of a Rapid Immunochromatographic Strip Test for the Detection of Mulberroside A. Phytochemical Analysis. 2015 Nov 1;26(6):423-427. https://doi.org/10.1002/pca.2576
Inyai, Chadathorn ; Komaikul, Jukrapun ; Kitisripanya, Tharita ; Tanaka, Hiroyuki ; Sritularak, Boonchoo ; Putalun, Waraporn. / Development of a Rapid Immunochromatographic Strip Test for the Detection of Mulberroside A. In: Phytochemical Analysis. 2015 ; Vol. 26, No. 6. pp. 423-427.
@article{5cec686039c844f4a5fd0d4483bf9829,
title = "Development of a Rapid Immunochromatographic Strip Test for the Detection of Mulberroside A",
abstract = "Introduction Mulberroside A (MuA) is the major active anti-tyrosinase compound in the root bark extract of Morus alba L. (Moraceae). Typically, MuA is widely employed as an active ingredient in whitening cosmetics. A rapid and simple assay system utilizing a small quantity of test sample is essential for the detection of MuA in large number of samples. An immunoassay using highly specific MuA polyclonal antibodies may be useful for the determination of small quantities of MuA in test samples. Objective To establish a rapid qualitative MuA test, an immunochromatographic strip test was developed using anti-MuA polyclonal antibodies (anti-MuA PAb). Methodology The qualitative assay was based on a competitive immunoassay where the detection reagent consisted of anti-MuA PAb colored with colloidal gold particles. The capture reagent was a MuA-ovalbumin (MuA-OVA) conjugate immobilized on the test strip membrane. Results A sample containing MuA and the detection reagent were incubated together with immobilized capture reagent on a nitrocellulose membrane. When MuA was present, it competed with the immobilized conjugates on the strip membrane to bind a limited amount of colored antibodies; thus, a positive sample showed no color on the capture spot zone. The detection limit for the strip test was 2 μg/mL. The developed immunochromatographic strip test was utilized to determine MuA in plants, medical preparations and cosmetic samples. Conclusion This immunochromatographic strip test is advantageous as a rapid, simple and sensitive screening method for the detection of MuA in plant extracts, cosmetic samples and pharmaceutical products. Mulberroside A (MuA) is the major active anti-tyrosinase compound in the root bark extract of Morus alba L. (Moraceae). Typically, MuA is widely employed as an active ingredient in whitening cosmetics. To establish a rapid qualitative MuA test, an immunochromatographic strip test was developed using anti-MuA polyclonal antibodies (anti-MuA PAb) colored with colloidal gold particles as the detector reagent. The capture reagent was a MuA-ovalbumin (MuA-OVA) conjugate immobilized on the test strip membrane. When MuA was present, it competed with the immobilized conjugates on the strip membrane to bind a limited amount of colored antibodies; thus, a positive sample showed no color on the capture spot zone. The detection limit for the strip test was 2 μg/ml. This immunochromatographic strip test is advantageous as a rapid, simple and sensitive screening method for the detection of MuA in plant extracts, cosmetic samples and pharmaceutical products.",
author = "Chadathorn Inyai and Jukrapun Komaikul and Tharita Kitisripanya and Hiroyuki Tanaka and Boonchoo Sritularak and Waraporn Putalun",
year = "2015",
month = "11",
day = "1",
doi = "10.1002/pca.2576",
language = "English",
volume = "26",
pages = "423--427",
journal = "Phytochemical Analysis",
issn = "0958-0344",
publisher = "John Wiley and Sons Ltd",
number = "6",

}

TY - JOUR

T1 - Development of a Rapid Immunochromatographic Strip Test for the Detection of Mulberroside A

AU - Inyai, Chadathorn

AU - Komaikul, Jukrapun

AU - Kitisripanya, Tharita

AU - Tanaka, Hiroyuki

AU - Sritularak, Boonchoo

AU - Putalun, Waraporn

PY - 2015/11/1

Y1 - 2015/11/1

N2 - Introduction Mulberroside A (MuA) is the major active anti-tyrosinase compound in the root bark extract of Morus alba L. (Moraceae). Typically, MuA is widely employed as an active ingredient in whitening cosmetics. A rapid and simple assay system utilizing a small quantity of test sample is essential for the detection of MuA in large number of samples. An immunoassay using highly specific MuA polyclonal antibodies may be useful for the determination of small quantities of MuA in test samples. Objective To establish a rapid qualitative MuA test, an immunochromatographic strip test was developed using anti-MuA polyclonal antibodies (anti-MuA PAb). Methodology The qualitative assay was based on a competitive immunoassay where the detection reagent consisted of anti-MuA PAb colored with colloidal gold particles. The capture reagent was a MuA-ovalbumin (MuA-OVA) conjugate immobilized on the test strip membrane. Results A sample containing MuA and the detection reagent were incubated together with immobilized capture reagent on a nitrocellulose membrane. When MuA was present, it competed with the immobilized conjugates on the strip membrane to bind a limited amount of colored antibodies; thus, a positive sample showed no color on the capture spot zone. The detection limit for the strip test was 2 μg/mL. The developed immunochromatographic strip test was utilized to determine MuA in plants, medical preparations and cosmetic samples. Conclusion This immunochromatographic strip test is advantageous as a rapid, simple and sensitive screening method for the detection of MuA in plant extracts, cosmetic samples and pharmaceutical products. Mulberroside A (MuA) is the major active anti-tyrosinase compound in the root bark extract of Morus alba L. (Moraceae). Typically, MuA is widely employed as an active ingredient in whitening cosmetics. To establish a rapid qualitative MuA test, an immunochromatographic strip test was developed using anti-MuA polyclonal antibodies (anti-MuA PAb) colored with colloidal gold particles as the detector reagent. The capture reagent was a MuA-ovalbumin (MuA-OVA) conjugate immobilized on the test strip membrane. When MuA was present, it competed with the immobilized conjugates on the strip membrane to bind a limited amount of colored antibodies; thus, a positive sample showed no color on the capture spot zone. The detection limit for the strip test was 2 μg/ml. This immunochromatographic strip test is advantageous as a rapid, simple and sensitive screening method for the detection of MuA in plant extracts, cosmetic samples and pharmaceutical products.

AB - Introduction Mulberroside A (MuA) is the major active anti-tyrosinase compound in the root bark extract of Morus alba L. (Moraceae). Typically, MuA is widely employed as an active ingredient in whitening cosmetics. A rapid and simple assay system utilizing a small quantity of test sample is essential for the detection of MuA in large number of samples. An immunoassay using highly specific MuA polyclonal antibodies may be useful for the determination of small quantities of MuA in test samples. Objective To establish a rapid qualitative MuA test, an immunochromatographic strip test was developed using anti-MuA polyclonal antibodies (anti-MuA PAb). Methodology The qualitative assay was based on a competitive immunoassay where the detection reagent consisted of anti-MuA PAb colored with colloidal gold particles. The capture reagent was a MuA-ovalbumin (MuA-OVA) conjugate immobilized on the test strip membrane. Results A sample containing MuA and the detection reagent were incubated together with immobilized capture reagent on a nitrocellulose membrane. When MuA was present, it competed with the immobilized conjugates on the strip membrane to bind a limited amount of colored antibodies; thus, a positive sample showed no color on the capture spot zone. The detection limit for the strip test was 2 μg/mL. The developed immunochromatographic strip test was utilized to determine MuA in plants, medical preparations and cosmetic samples. Conclusion This immunochromatographic strip test is advantageous as a rapid, simple and sensitive screening method for the detection of MuA in plant extracts, cosmetic samples and pharmaceutical products. Mulberroside A (MuA) is the major active anti-tyrosinase compound in the root bark extract of Morus alba L. (Moraceae). Typically, MuA is widely employed as an active ingredient in whitening cosmetics. To establish a rapid qualitative MuA test, an immunochromatographic strip test was developed using anti-MuA polyclonal antibodies (anti-MuA PAb) colored with colloidal gold particles as the detector reagent. The capture reagent was a MuA-ovalbumin (MuA-OVA) conjugate immobilized on the test strip membrane. When MuA was present, it competed with the immobilized conjugates on the strip membrane to bind a limited amount of colored antibodies; thus, a positive sample showed no color on the capture spot zone. The detection limit for the strip test was 2 μg/ml. This immunochromatographic strip test is advantageous as a rapid, simple and sensitive screening method for the detection of MuA in plant extracts, cosmetic samples and pharmaceutical products.

UR - http://www.scopus.com/inward/record.url?scp=84944354750&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84944354750&partnerID=8YFLogxK

U2 - 10.1002/pca.2576

DO - 10.1002/pca.2576

M3 - Article

C2 - 26096098

AN - SCOPUS:84944354750

VL - 26

SP - 423

EP - 427

JO - Phytochemical Analysis

JF - Phytochemical Analysis

SN - 0958-0344

IS - 6

ER -