Development of a sensitive ELISA for human leptin, using monoclonal antibodies

Keiichi Imagawa, Yayoi Matsumoto, Yoshito Numata, Atsushi Morita, Shino Kikuoka, Mikio Tamaki, Chie Higashikubo, Tetsuo Tsuji, Kazuyuki Sasakura, Hiroshi Teraoka, Hiroaki Masuzaki, Kiminori Hosoda, Yoshihiro Ogawa, Kazuwa Nakao

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

A new, sensitive ELISA for human leptin in plasma and cerebrospinal fluid (CSF) was developed, using monoclonal antibodies. The lower limit of detection of this ELISA was 0.78 pg/assay. Both intra- and interassay imprecision values were <7%. The dilution curves of plasma and CSF showed good linearity, and the recovery was 83.2-95.6%. There was good correlation between plasma leptin concentrations by the ELISA and a commercially available RIA (r = 0.99). Our ELISA is advantageous because it does not require radioisotopes, it produces results in hours rather than days, and more importantly, it improves on the detection limit and plasma interference of the RIA kit. The new ELISA enables measurement of low concentrations of leptin, as are seen in CSF and in plasma of patients with anorexia nervosa.

Original languageEnglish
Pages (from-to)2165-2171
Number of pages7
JournalClinical Chemistry
Volume44
Issue number10
Publication statusPublished - 1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical

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