Development of a sensitive ELISA for human leptin, using monoclonal antibodies

Keiichi Imagawa, Yayoi Matsumoto, Yoshito Numata, Atsushi Morita, Shino Kikuoka, Mikio Tamaki, Chie Higashikubo, Tetsuo Tsuji, Kazuyuki Sasakura, Hiroshi Teraoka, Hiroaki Masuzaki, Kiminori Hosoda, Yoshihiro Ogawa, Kazuwa Nakao

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A new, sensitive ELISA for human leptin in plasma and cerebrospinal fluid (CSF) was developed, using monoclonal antibodies. The lower limit of detection of this ELISA was 0.78 pg/assay. Both intra- and interassay imprecision values were <7%. The dilution curves of plasma and CSF showed good linearity, and the recovery was 83.2-95.6%. There was good correlation between plasma leptin concentrations by the ELISA and a commercially available RIA (r = 0.99). Our ELISA is advantageous because it does not require radioisotopes, it produces results in hours rather than days, and more importantly, it improves on the detection limit and plasma interference of the RIA kit. The new ELISA enables measurement of low concentrations of leptin, as are seen in CSF and in plasma of patients with anorexia nervosa.

Original languageEnglish
Pages (from-to)2165-2171
Number of pages7
JournalClinical Chemistry
Volume44
Issue number10
Publication statusPublished - Oct 12 1998
Externally publishedYes

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Leptin
Cerebrospinal fluid
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Plasmas
Cerebrospinal Fluid
Limit of Detection
Anorexia Nervosa
Radioisotopes
Dilution
Assays
Recovery

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Imagawa, K., Matsumoto, Y., Numata, Y., Morita, A., Kikuoka, S., Tamaki, M., ... Nakao, K. (1998). Development of a sensitive ELISA for human leptin, using monoclonal antibodies. Clinical Chemistry, 44(10), 2165-2171.

Development of a sensitive ELISA for human leptin, using monoclonal antibodies. / Imagawa, Keiichi; Matsumoto, Yayoi; Numata, Yoshito; Morita, Atsushi; Kikuoka, Shino; Tamaki, Mikio; Higashikubo, Chie; Tsuji, Tetsuo; Sasakura, Kazuyuki; Teraoka, Hiroshi; Masuzaki, Hiroaki; Hosoda, Kiminori; Ogawa, Yoshihiro; Nakao, Kazuwa.

In: Clinical Chemistry, Vol. 44, No. 10, 12.10.1998, p. 2165-2171.

Research output: Contribution to journalArticle

Imagawa, K, Matsumoto, Y, Numata, Y, Morita, A, Kikuoka, S, Tamaki, M, Higashikubo, C, Tsuji, T, Sasakura, K, Teraoka, H, Masuzaki, H, Hosoda, K, Ogawa, Y & Nakao, K 1998, 'Development of a sensitive ELISA for human leptin, using monoclonal antibodies', Clinical Chemistry, vol. 44, no. 10, pp. 2165-2171.
Imagawa K, Matsumoto Y, Numata Y, Morita A, Kikuoka S, Tamaki M et al. Development of a sensitive ELISA for human leptin, using monoclonal antibodies. Clinical Chemistry. 1998 Oct 12;44(10):2165-2171.
Imagawa, Keiichi ; Matsumoto, Yayoi ; Numata, Yoshito ; Morita, Atsushi ; Kikuoka, Shino ; Tamaki, Mikio ; Higashikubo, Chie ; Tsuji, Tetsuo ; Sasakura, Kazuyuki ; Teraoka, Hiroshi ; Masuzaki, Hiroaki ; Hosoda, Kiminori ; Ogawa, Yoshihiro ; Nakao, Kazuwa. / Development of a sensitive ELISA for human leptin, using monoclonal antibodies. In: Clinical Chemistry. 1998 ; Vol. 44, No. 10. pp. 2165-2171.
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