TY - JOUR
T1 - Development of a simple and rapid method for the detection of isomiroestrol in Pueraria candollei by an immunochromatographic strip test
AU - Chaingam, Jiranan
AU - Kitisripanya, Tharita
AU - Krittanai, Supaluk
AU - Sakamoto, Seiichi
AU - Tanaka, Hiroyuki
AU - Putalun, Waraporn
N1 - Funding Information:
Acknowledgements This work was supported by The Thailand Research Fund (IRN61W0005) and Faculty of Pharmaceutical Sciences, Khon Kaen University, Thailand. The authors thank Dr. Orawan Monthakantirat, Faculty of Pharmaceutical Sciences, Khon Kaen University for providing the authentic standard of isomiroestrol.
PY - 2019/6/14
Y1 - 2019/6/14
N2 - Pueraria candollei (P. candollei) is a traditional Thai herb widely used for estrogen replacement therapy because it contains many unique chromenes that possess potent estrogenic activity, one of which is known as isomiroestrol. Since isomiroestrol is a promising compound that is solely present in P. candollei, it can be used as an identifying marker for standardization of P. candollei. Here, we developed a lateral-flow immunochromatographic strip (ICS) test using a colloidal gold nanoparticle-conjugated anti-isomiroestrol monoclonal antibody (12C1-mAb) for the detection of isomiroestrol in plant samples and products of P. candollei. The advantages of the developed ICS over an enzyme-linked immunosorbent assay are its simplicity and rapidity, as the ICS test can be completed 15 min after dipping the strip into the analyte solution. The detectable concentration of isomiroestrol was 7.0 µg/mL. Considering the demand for the standardization of P. candollei due to concerns regarding its quality, our ICS test using isomiroestrol as an identifying marker would be effective and useful to assess the presence of isomiroestrol.
AB - Pueraria candollei (P. candollei) is a traditional Thai herb widely used for estrogen replacement therapy because it contains many unique chromenes that possess potent estrogenic activity, one of which is known as isomiroestrol. Since isomiroestrol is a promising compound that is solely present in P. candollei, it can be used as an identifying marker for standardization of P. candollei. Here, we developed a lateral-flow immunochromatographic strip (ICS) test using a colloidal gold nanoparticle-conjugated anti-isomiroestrol monoclonal antibody (12C1-mAb) for the detection of isomiroestrol in plant samples and products of P. candollei. The advantages of the developed ICS over an enzyme-linked immunosorbent assay are its simplicity and rapidity, as the ICS test can be completed 15 min after dipping the strip into the analyte solution. The detectable concentration of isomiroestrol was 7.0 µg/mL. Considering the demand for the standardization of P. candollei due to concerns regarding its quality, our ICS test using isomiroestrol as an identifying marker would be effective and useful to assess the presence of isomiroestrol.
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U2 - 10.1007/s11418-019-01307-6
DO - 10.1007/s11418-019-01307-6
M3 - Article
C2 - 30976950
AN - SCOPUS:85064213877
VL - 73
SP - 577
EP - 583
JO - Journal of Natural Medicines
JF - Journal of Natural Medicines
SN - 1340-3443
IS - 3
ER -