Abstract
Surface plasmon resonance (SPR) biosensors were used to develop a rapid and simultaneous detection method for three important foodborne pathogens, i.e., Escherichia coli O157:H7 (O157), Salmonella Enteritidis (SE), and Listeria monocytogenes (LM). Bacterial homogenates prepared by sonication from bacterial suspensions at various cell concentrations were analyzed using SPR biosensors and sensor chips with polyclonal antibodies specific to each of the target pathogens. The precipitates from the homogenates were demonstrated to be suitable for specific and simultaneous detection of O157, SE, and LM. By using the precipitates and a custom-built multichannel SPR biosensor, the lower detection limit for O157, SE, and LM was determined to be 0.6 × 106, 1.8 × 106, and 0.7 × 107 CFU/mL, respectively, in the presence of non-target pathogens at concentrations of 105 to 108 CFU/mL.
| Original language | English |
|---|---|
| Pages (from-to) | 317-325 |
| Number of pages | 9 |
| Journal | Food Science and Technology Research |
| Volume | 20 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jan 1 2014 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Food Science
- Chemical Engineering(all)
- Industrial and Manufacturing Engineering
- Marketing
Cite this
Development of a simultaneous detection method for foodborne pathogens using surface plasmon resonance biosensors. / Zhang, Xiaoguang; Kitaoka, Hayato; Tsuji, Sachiko; Tamai, Mitsuru; Kobayashi, Hiroshi; Honjoh, Ken-ichi; Miyamoto, Takahisa.
In: Food Science and Technology Research, Vol. 20, No. 2, 01.01.2014, p. 317-325.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of a simultaneous detection method for foodborne pathogens using surface plasmon resonance biosensors
AU - Zhang, Xiaoguang
AU - Kitaoka, Hayato
AU - Tsuji, Sachiko
AU - Tamai, Mitsuru
AU - Kobayashi, Hiroshi
AU - Honjoh, Ken-ichi
AU - Miyamoto, Takahisa
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Surface plasmon resonance (SPR) biosensors were used to develop a rapid and simultaneous detection method for three important foodborne pathogens, i.e., Escherichia coli O157:H7 (O157), Salmonella Enteritidis (SE), and Listeria monocytogenes (LM). Bacterial homogenates prepared by sonication from bacterial suspensions at various cell concentrations were analyzed using SPR biosensors and sensor chips with polyclonal antibodies specific to each of the target pathogens. The precipitates from the homogenates were demonstrated to be suitable for specific and simultaneous detection of O157, SE, and LM. By using the precipitates and a custom-built multichannel SPR biosensor, the lower detection limit for O157, SE, and LM was determined to be 0.6 × 106, 1.8 × 106, and 0.7 × 107 CFU/mL, respectively, in the presence of non-target pathogens at concentrations of 105 to 108 CFU/mL.
AB - Surface plasmon resonance (SPR) biosensors were used to develop a rapid and simultaneous detection method for three important foodborne pathogens, i.e., Escherichia coli O157:H7 (O157), Salmonella Enteritidis (SE), and Listeria monocytogenes (LM). Bacterial homogenates prepared by sonication from bacterial suspensions at various cell concentrations were analyzed using SPR biosensors and sensor chips with polyclonal antibodies specific to each of the target pathogens. The precipitates from the homogenates were demonstrated to be suitable for specific and simultaneous detection of O157, SE, and LM. By using the precipitates and a custom-built multichannel SPR biosensor, the lower detection limit for O157, SE, and LM was determined to be 0.6 × 106, 1.8 × 106, and 0.7 × 107 CFU/mL, respectively, in the presence of non-target pathogens at concentrations of 105 to 108 CFU/mL.
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U2 - 10.3136/fstr.20.317
DO - 10.3136/fstr.20.317
M3 - Article
VL - 20
SP - 317
EP - 325
JO - Food Science and Technology Research
JF - Food Science and Technology Research
SN - 1344-6606
IS - 2
ER -