Development of a simultaneous detection method for foodborne pathogens using surface plasmon resonance biosensors

Xiaoguang Zhang, Hayato Kitaoka, Sachiko Tsuji, Mitsuru Tamai, Hiroshi Kobayashi, Ken-ichi Honjoh, Takahisa Miyamoto

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Surface plasmon resonance (SPR) biosensors were used to develop a rapid and simultaneous detection method for three important foodborne pathogens, i.e., Escherichia coli O157:H7 (O157), Salmonella Enteritidis (SE), and Listeria monocytogenes (LM). Bacterial homogenates prepared by sonication from bacterial suspensions at various cell concentrations were analyzed using SPR biosensors and sensor chips with polyclonal antibodies specific to each of the target pathogens. The precipitates from the homogenates were demonstrated to be suitable for specific and simultaneous detection of O157, SE, and LM. By using the precipitates and a custom-built multichannel SPR biosensor, the lower detection limit for O157, SE, and LM was determined to be 0.6 × 106, 1.8 × 106, and 0.7 × 107 CFU/mL, respectively, in the presence of non-target pathogens at concentrations of 105 to 108 CFU/mL.

Original languageEnglish
Pages (from-to)317-325
Number of pages9
JournalFood Science and Technology Research
Volume20
Issue number2
DOIs
Publication statusPublished - 2014

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering
  • Marketing

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