Development of a simultaneous two-dimensional micro-HPLC analysis system for the chiral amino acid metabolomics study in mammals

Yurika Miyoshi, Yosuke Tojo, Hai Han, Masashi Mita, Kenji Hamase

Research output: Contribution to journalArticle

Abstract

D-Amino acids, enantiomers of L-amino acids, are increasingly recognized as being novel physiologically active substances and biomarkers in higher animals, including human beings. Although L-amino acids are abundantly present in the tissues and physiological fluids, the amounts of their enantiomers, D-forms, are extremely small in most cases. Therefore, for the precise analysis of small amounts of D-amino acids, a highly sensitive and selective analytical method is essential. In the present article, a two-dimensional micro-HPLC procedure combining a reversed-phase column and an enantioselective column is described for chiral amino acid metabolomics studies in mammals.

Original languageEnglish
Pages (from-to)489-499
Number of pages11
Journalbunseki kagaku
Volume61
Issue number6
DOIs
Publication statusPublished - Jan 1 2012

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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