Development of a stable isotope dilution assay for the quantification of 5-methyl-(E)-2-hepten-4-one: Application to hazelnut oils and hazelnuts

P. Pfnuer, Toshiro Matsui, W. Grosch, H. Guth, T. Hofmann, P. Schieberle

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

A stable isotope dilution assay was developed for the quantitation of the hazelnut odorant 5-methyl-(E)-2-hepten-4-one by mass chromatography using synthesized [2H]2-5-methyl-(E)-2-hepten-4-one as the internal standard. Application of the method on two batches of commercial hazelnut oils, processed from either roasted or unroasted nuts, revealed 6.4 μg 5-methyl- (E)-2-hepten-4-one per kg of unroasted oil whereas 315.8 μg per kg was determined in the roasted nut oil. The about 50-fold higher amount of 5- methyl-(E)-2-hepten-4-one in roasted hazelnut oil suggested the necessity of a thermal treatment to generate the flavor compound. Pan frying of raw hazelnuts (9 to 15 min) or boiling of the crushed nut material for 1 h in water led to an increase of 5-methyl-(E)-2-hepten-4-one by factors of 600 and 800, respectively, thereby corroborating that the major part of the nut flavorant is formed during heat treatment from a yet unknown precursor in hazelnuts.

Original languageEnglish
Pages (from-to)2044-2047
Number of pages4
JournalJournal of Agricultural and Food Chemistry
Volume47
Issue number5
DOIs
Publication statusPublished - May 1 1999

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hazelnut oil
Corylus
isotope dilution technique
hazelnuts
Nuts
Isotopes
Dilution
stable isotopes
Assays
Oils
assays
nut oils
heat treatment
flavor compounds
boiling
application methods
odor compounds
Flavor compounds
Hot Temperature
Heat treatment

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Development of a stable isotope dilution assay for the quantification of 5-methyl-(E)-2-hepten-4-one : Application to hazelnut oils and hazelnuts. / Pfnuer, P.; Matsui, Toshiro; Grosch, W.; Guth, H.; Hofmann, T.; Schieberle, P.

In: Journal of Agricultural and Food Chemistry, Vol. 47, No. 5, 01.05.1999, p. 2044-2047.

Research output: Contribution to journalArticle

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